Introduction to bioinformatics - What If

dasypygalstockingsBiotechnology

Oct 2, 2013 (4 years and 10 days ago)

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Bioinformatics

Necessary evil, panacea, or just a
useful tool?

With a month in the lab you can
easily prevent having to sit an
hour in front of the computer.

Nothing is impossible for a
biologist who doesn’t have to
discover it him/her
-
self.



Bio + informatica

Humaan genoom




Humaan genoom


....acccaa gaagtcagaa tcctcgaagc tgaagcctga ctgtaagctc tgcctcctac

aactagacaa gtcacaatgc aacccctttg ctccagagga aaaggagtaa gcacagaggg

cttctgagtc cggcaatgct ccatttcctg acaggagcat tttacagact tgcgtctgcc

cctctgaggg aggcagggcc atcagctaac actcagagac aaggatggtc ctacaacatc

ctgatctggg aggaagaggt gaaggcaact caccaccctc aggcatctta acagaaaaca

gtggaactaa ggctcattct aaagaatgtg cccaagatca cacaccgaag gctgcaaagc

gagagtcaaa cttggaaccc tgaacctgca cttgtccctt tacactgccc tgcagcatgg

atgcgatggc tgggcagagg gaagtgccca agcctgggga caaaaatgcc tacctg....

Chromosomes




Genome annotation




Bioinformatics

and medicines


One day we know everything
about all human (and flu)
proteins and then can we start
to ‘calculate’ flu
-
medicines.

Drug Design




GPCR

Drug Design




Mens vs parasiet

Parasite

Active site

Medicine

Every small molecule is, in
principle, a poison. We call it a
flu
-
medicine if it kills the flu
much faster than the patient.


H1N1 / H5N1

H1N1

Strange mortality 1918

(the 1918 flu was H1N1)

Same strange mortality
in Mexican H1N1? Why?

Flu bioinformatics

Determine which variant it is
from sequence comparisons

Design medicine(s) (tamivir, etc)

Determine why H1N1 is
dangerous

Predict next variant



Bird/pig flu

Pig flu pandemic?

H5N1

(but H1N1 also went through birds)


Neuraminidase

Quote from Wikipedia/WHO/etc:

On February 27, 2005, a 14
-
year
-
old Vietnamese girl was
documented to be carrying an H5N1 influenza virus strain
that was resistant to the drug oseltamivir. However, the
Vietnamese girl who had received a prophylactic dose (75
mg once a day) was found to be non
-
responsive to the
medication. In growing fears of a global avian flu pandemic,
scientists began to look for a cause of resistance to the
Tamiflu medication. The cause was determined to be a
histidine
-
to
-
tryosine substitution at position 274 in its
neuraminidase protein

H1N1
medicijn