Russell W. Carlson - the Complex Carbohydrate Research Center ...

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Feb 12, 2013 (4 years and 6 months ago)

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Russell W. Carlson



A.
ADMINISTRATION



B. INSTRUCTION



Courses taught


BCMB 3100
-

110

students
-

3 contact hours per week for
12

weeks (Fall Semester
2002
)

B
CBM

8
02
0


7 contact hours per week (April 2002
)


Undergraduate Research Students


Biochemis
try 4960

Matthew Hilley (spring 2002
)

Biochemistry 4960

Russ A
yers (spring 2002
)

Biochemistry 4960

Kahtonna Allen (spring 2002)

Biochemistry 4960

Miranda Walker (spring 2002)

Biochemistry 4960

James Russ
ell

(spring 2002)

Biochemistry 4960

Christy Rockwell
(fall 2002)


Graduate Students


Vinata Vedam

Microbiology

Hye
-
Jung Park

Biochemistry

and Molecular Biology

Kahtonna Allen

Biochemistry

and Molecular Biology


Postdoctoral Research Associates


Biswa Choudhury

Wim D’Haeze

Anup Datta

Scott Forsberg

Brent R
idley


Research Faculty


Ben Jeyaretnam


Dissertation Committees


Cristina De Meo

Chemistry (Ph.D.)


Spring 2002

Dawn Adin


Microbiology (Ph.D.)

Patrick Curtis


Microbiology (Ph.D.)

Katharina Lohmann

Veterinary Medicine (Ph.D.)

Debatosh Majumdar

Chemistr
y (Ph.D.)


Russell W. Carlson


2


C.


RESEARCH

AND OTHER CREATIVE ACTIVITY
:


Publications

(all refereed journals)


Dean, C.R., A. Datta, R.W. Carlson, and J.B. Goldberg. 2002. WbjA adds glucose to
complete the O
-
antigen trisaccharide repeating unit of the lipopolysaccharide

of
Pseudomonas aeruginosa

Serogroup O11.
J.Bacteriol
. 184:323
-
326.

Shafer, W.M., A. Datta, V.S.K. Kolli, M.M. Rahman, J.T. Balthazar, L.E. Martin, W.L. Veal,
D.S. Stepehens, and R. Carlson. 2002. Phase variable changes in genes
lgtA

and
lgtC

within th
e
lgtABCDE

operon of Neisseria gonorrhoeae can modulate gonococcal
susceptibility to normal human serum.
J. Endotoxin Res
. 8:47
-
58 .

Tzeng, Y.
-
L., A. Datta, V.S.K. Kolli, R.W. Carlson, and D.S. Stephens. 2002. Endotoxin of
Neisseria meningitidis

compose
d only of intact lipid A: Inactivation of the meningococcal
3
-
deoxy
-
D
-
manno
-
octulosonic acid (Kdo) transferase.
J. Bacteriol
. 184:2379
-
2388.

Tzeng, Y.
-
L., A.K. Datta, C. Strole, V.S. K. Kolli, M.R. Brick, W.P. Taylor, R.W. Carlson, R.W.
Woodard and D.S.

Stephens. 2002. KpsF is the arabiose 5
-
phosphate isomerase
required for 3
-
deoxy
-
D
-
manno
-
octulosonic acid biosynthesis and for both
lipooligosaccharide assembly and capsular polysaccharide expression in of
Neisseria
meningitides.

J. Biol. Chem.

277:241
03
-
24113.

Jeyaretnam, B., J. Glushka, V.S.K. Kolli, and R.W. Carlson. 2002. Characterization of a
novel lipid
-
A from
Rhizobium

species Sin
-
1: A unique lipid
-
A structure that is devoid of
phosphate and has a glycosyl backbone consisting of glucosamine an
d 2
-
aminogluconic
acid.
J. Biol. Chem.

277:41802
-
41810.

Vandenplas, M.L., R.W. Carlson, B.S. Jeyaretnam, B. McNeill, M.H. Barton, N. Norton, T.F.
Murray, and J.N. Moore. 2002.
Rhizobium

sin
-
1 LPS prevents enteric LPS
-
induced
cytokine production by mono
Mac 6 cells.
J. Biol. Chem
.. 277:41811
-
41816.

Loh, J., R.W. Carlson, W.S. York, and G. Stacey. 2002. Bradyoxetin, a unique chemical
signal involved in symbiotic gene regulation.”
PNAS.

99:14446
-
14451.

Vedam, V., E.L.Kannenberg, J.G. Haynes, D.J. Sher
rier, A. Datta, and R.W. Carlson. 2002.
A
Rhizobium leguminosarum

AcpXL mutant produces lipopolysaccharide lacking 27
-
hydroxyoctacosanoic acid.”
J. Bacteriol
. 185
. In press.



Collaborations


Within the CCRC


Russell W. Carlson


3


Between CCRC Personnel and Investigators at the University of Georgia



Russell W. Carlson, Geert
-
Jan Boons,
with James Moore and Michelle Vandenplaas
(Departmen
t of Large Animal Medicine, College of Veterinary Medicine). Synthesis and
evaluation of novel endotoxin antagonists.


Between CCRC Personnel and Organizations in the State of Georgia

(not including
University of Georgia)

Russell W. Carlson and Anup Datta

with David S. Stephens (Department of Medic
al

Microbiology, and Immunology, Emory University, Atlanta). Structural characterization of
Neisseria menigitidis lipooligosaccharides.

Russell W. Carlson and
A
nup Datta with David S. Stephens (Department of

Medic
al
Microbiology, and Immunology, Emory University, Atlanta)
,
Structural characterization of
Neisseria menigitidis
capsular polysaccharides
.

Russell W. Carlson and Anup Datta with David S. Stephens and William Shafer
. Structural
characterization of Neisseria gonohoreae lipooligosaccharides.

Russell W. Carlson with David Stephens
(Department of Medic
al

Microbiology, and
Immunology, Emory University, Atlanta)

and Conrad Qu
inn (Infectious Diseases, Centers for
Disease Control).

Characterization of the cell surface polysaccharides from
Bacillus

anthracis
.


Within Academia and Government

Russell W. Carlson with Elmar L. Kannenberg (Microbiology/Biotechnology, University of

bingen, Germany). Structural characterization of an LPS epitope that is specific in
Rhizobium leguminosarum bv. viciae bacteroids.

Russell W. Carlson with Dale Noel (Department of Biology, Marquette University, Milwaukee,
WI). Structural and functional a
nalysis of Rhizobium etli lipopolysaccharides in legume
symbiosis.

Russell W. Carlson, John Glushka,
with Gary Stacey (Department of Microbiology, University
of Tennessee, Knoxville). Structural determination of signal molecules require
d for
Bradyrhizobium japonicum
-
soybean symbiosis.

Russell W. Carlson with C. Tsai (Division of Bacterial Products, FDA Center for Biologics
Evaluation and Research) and David Lin (Division of Allergenic Products and Parasitology,
FDA
-
CBER, National Insti
tutes of Health, Bethesda, MD). Structural analysis of non
-
typable
Hemophilus influenza
e

lipooligosaccharide.

Russell W. Carlson and
A
nup Datta with Graham Walker (Biology Department,
Massachusetts Institute of Technology).

Analysis of the lipid
-
A from
S
inorhizobium meliloti
.

Russell W. Carlson and
A
nup Datta with Graham Walker (Biology Department,
Massachusetts Institute of Technology)

and Marty Roop (Department of Microbiology, East
Carolina University)
. Analysis of the lipid
-
A from
Brucella abortus
.

R
ussell
W.

Carlson,

Anup

Datta

and

Mu
-
Yun

Gao,

with

Jos

Vanderley

(Biology

Department,

Lueven

University,

Belgium).

A
nalysis

of

the

LPS

from

R.

etli

CE3

mutants.

Russell

W.

Carlson,

Anup

Datta,

with

Jan

Kijne

(P
l
ant

Biology,

Leiden

University,

The

Netherla
nds).

Analysis

of

the

capsular

polysaccharides

from

R.

leguminosarum
.

Russell W. Carlson


4





Invited presentations


North American
C
onference on Symbiotic Nir
t
rogen Fixation, Columbia, MO., June, 2002



Professional Meetings Attended


American Society for Microbiology, Sal
t Lake City, Utah, May 19
-
23, 2002

North American Conference on Symbiotic Nitrogen Fixation, Columbia, MO, June 3
-
8, 2002

International Endotoxin Society, Washington d. C., July 18
-
21, 2002




Abstracts


Vedam, V., E. Kannenberg, B. Ridley, and R.W. Carlso
n. “A study of the symbiotic and
biosynthetic function of the 27
-
OHC28:0 fatty acid component of the lipid
-
A of
Rhizobium
leguminosarum

3841. 102
nd

General Meeting of the American Society for Microbiology,
Salt Lake City, Utah, May 19
-
23, 2002.

Tzeng,
Y., A. Datta, C.A. Strole, M.R. Birck, W.P. Taylor, R.W. Carlson, R.S. Woodard, D.S.
Stephens. “KpsF is the arabinose 5
-
phosphate isomerase required for 3
-
deoxy
-
n
D
-
manno
-
ociulosonic acid (Kdo) biosynthesis and for both lipooligosaccharide assembly
and cap
sular polysacchardie expression in Neisseria meningitidis.” 102
nd

General
Meeting of the American Society for Microbiology, Salt Lake City, Utah, May 19
-
23, 2002.

Datta, A., T.
-
L. Tzeng, V.K. Kolli, C.Strole, M.R. Birck, W.P. Taylor, R.W. Woodard, R.W.
Ca
rlson, D.S. Stephens. “Viable
Neisseria meningitidis

mutants that express only lipid
A.” 7
th

Conference of the International Endotoxin Society, Washington, D. C., July 18
-
21,
2002.

Jeyaretnam, B., J. Glushka, R.W. Carlson. “Characterization of novel rhi
zobial lipid A
structures.” 7
th

Conference of the International Endotoxin Society, Washington, D. C.,
July 18
-
21, 2002.

Zughaier, S.M., S.M. Zimmer, A. Data, Y.
-
L. Tzeng, R.W. Carlson, D.S. Stephens. “LPS
binding protein (LBP) can attenuate early signal
responses to cell
-
bound LPS.” 7
th

Conference of the International Endotoxin Society, Washington, D. C., July 18
-
21, 2002.


Grants held


Active:

Title:
Rhizobial Lipopolysaccharides Essential for Infection

Agency: NIH/NIGMS

Grant Number: 2RO1GM039583
-
1
5

Project Period: 06/01/88
-

03/31/06

Current Year Award: $306,392

Role: Principal Investigator

Russell W. Carlson


5


Title: Meningococcal Lipooligosaccharide & mucosal pathogenesis

Agency: NIH/Emory University subcontract to UGA

Grant Number: 5RO1AI33517
-
09

Project Peri
od: 01/01/97
-

12/31/02

Current Year Award: no
-
cost extension

Role: Co
-
Principal Investigator

Russell W. Carlson


6



Title:
Sinorhizobium

Lipopolysaccharide Structures Essential for Symbiotic Infection

Agency: USDA

Grant Number: 99
-
35305
-
8143

Project Period: 09/01/99

-

08/31/03

Current Year Award: $70,000

Role: Co
-
Principal Investigator


Title: Synthesis and evaluation of novel endotoxin antagonists

Agency: NIH/NIGMS

Grant Number: 5 RO1 GM61761
-
03

Project Period: 07/01/00
-
06/30/05

Current Year Award: $289,600

R
ole: Co
-
Principal Investigator


Title: Synthesis of
P. aeruginosa

LPS and its role in infection

Agency: NIH/NIAID/Univ of Virginia subcontract to UGA
$23,049 Carlson’s annual direct
costs

Grant Number: 1RO1AI50230
-
01

Project Period: 09/17/01
-
07/31/06

C
urrent Year Award: $33,375

Role: Co
-
Investigator


Pending:

Title: Meningococcal Lipooligosaccharide & mucosal pathogenesis

Agency: NIH/Emory University subcontract to UGA

Grant Number: 5RO1AI33517

Project Period: 01/01/03
-

12/31/07

(Awarded)

To
tal Funds Requested: $473,017

Role: Co
-
Principal Investigator


Title:
Bacillus Anthacis Cell Surface Carbohydrates

Agency: NIH

Grant Number: unknown

Project Period: 07/01/03


06/30/05

Total Funds Requested: $588,800

Role: Principal Investigator


D
.

PROFESSIONAL ACTIVITIES


Positions held in professional organizations


Member, International Endotoxin Society Executive Council

Member, Sigma Xi

Member, American Society for Microbiology

Member, American Society for Plant Physiology

Member, American Ch
emical Society

Russell W. Carlson


7



Reviews for
grants



Biotechnology and Biological Sciences Research Council Research Grant

National Institute of Health, Division of Intramural Research

U. S. Department of Agriculture, Plant Biochemistry

U.S. Department of Veterans Affai
rs

The Wellcome Trust



Reviews for journals


Applied
and

Environmental Microbiology (19
)


Editorial Board

Jan
.

2002
-
Dec
.

2004

Molecular Plant
-
Microbe Interactions

Molecular Microbiology

Phytochemistry

European Journal of Biochemistry

Carbohydrate Researc
h

(2)

Molecular & General Genomics

Canadian Journal of Chemistry

BioTechniques

Journal of Bacteriology


E.

UNIVERSITY GOVERNANCE



F.

OTHER CONTRIBUTIONS


Executive
Technical Director of the analytical services at the CCRC.