Microbiology Lab BT1207

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Feb 12, 2013 (4 years and 9 months ago)

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AKCE/PM/C
-
V/PRN/7.5.1/21/F
-
01

BT 1207

MICROBIOLOGY LAB





0 0 4 100


AIM


The course aims to develop the skills of students in area of microbiology.
Here hands on training is offered for the students to study microbes, their
identifications & characterization and their practical u
ses.


OBJECTIVES


At the end of the course the student would have learnt about the culturing
of microorganism, their identification using simple staining techniques.
Thus this hands on training will be very beneficial for them to undertake
project work in

the later semesters.




1.

Laboratory safety and sterilization techniques

2.

Microscopic methods in the identification of microorganisms

3.

Preparation of culture media


nutrient broth and nutrient agar

4.

Culturing of microorganisms


in broth and in plates ( pour
plates, streak plates,
isolation and preservation of bacterial cultures)

5.

Staining techniques


grams’ and differential

6.

Quantitation of microorganisms.

7.

Effect of disinfectants on microbial flora

8.

Isolation and identification of microorganisms from different
sources


soil, water
and milk

9.

Antibiotic sensitivity assay

10.

Growth curve


observation and growth characteristics of bacteria and yeast.

11.

Effect of different parameters on bacterial growth (ph, temperature & UV
irradiation)




Text Book:


1.

James.G.Cappuc
ino. Notalie Sherman. “Microbiology. A Laboratory Manual”,
Fourth edition, 1996, Addison Wesley Publishes.


Reference:


1.

P.Gunasekaran et.al. “Laboratory Manual in Microbiology” , 1995, New Age
International (P) limited publishers.

2.

Kannan. N, 1995. Laborato
ry manual in General Microbiology, Palani paramount
publications, Palani.










AKCE/PM/C
-
V/PRN/7.5.1/21/F
-
01

ARULMIGU KALASALINGAM COLLEGE OF ENGINEERING

ANAND NAGAR, KRISHNANKOIL


626 190

DEPARTMENT OF BIOTECHNOLOGY

MICROBIOLOGY LAB

COURSE PLAN
-

2006
-
2007

Name of the staff :Mr.R.Kasi
mani







Year : II

Subject / Code: Microbiology lab / BT1207



Sem/ Branch : III/BT


1. Objective:



To know the about the basic microbial techniques.



To develop the isolation, identification and preservation of Microorganisms.



To learn the safety guid
elines in handling microbes.



To apply the principles in identifying pathogenic and non pathogenic organisms
from different environment.


2. Prerequisite
:


A basic knowledge on BT 1204


3. Learning outcome and use
:



At the end of the course students
will be able to handle microbes without
contamination.



They will be able to identify and characterize the microbes.


4. Safety and precautions:

Students should be careful in handling microbes and avoid contamination.


5. Methods of assessment
:

Continuous e
valuation is done based on the performance in each laboratory
session (includes viva voce, performance, result analysis, and record submission)


6. Lesson plan
:


Topic
No.

Topic Name

Text Book

No. of
Periods

Cummulative
Hours

1.


2.



3.



4.



Laboratory safety and sterilization
Techniques(Lecture / Demonstration)

Microscopic methods in the identification
of microorganisms


Preparation of culture media


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4



4



4





4



8












AKCE/PM/C
-
V/PRN/7.5.1/21/F
-
01


5.



6.


7.





8.




9.


10.



11.


12.


13.


Isolation and preservation of bacterial
culture


Gram’s staining, Simple staining


Biochemical tests
:

1.

IMViC Tests

2.

Starch hydrolysis test

3.

Carbohydrate fermentation test


Quantitation of microorganisms


A)

Counting microscopy, Turbidometry


B) Serial dilution plating


Effect of disinfectants on microbial flora


Isolation and identification of
microorganisms from soil, Water and milk


Antibiotic disc sensitivity test


Growth curve of bacteria and yeast


E
ffect of different parameters on bacterial
growth(pH, Temperature, UV irradiation)


T1,R1,R2



T1,R2


T1,R1,R2





T1,R1,R2




T1,R1


T1,R1



T1,R1,R2


T1,R1,R2


T1,R2



4



4



4

4

4



4

4


4



4


4


4


4




16



20



24

28

32



36

40


44



48


52


56


60



Text Book:


1.

James.G.Cappucino. Notalie Sherman. “Microbiology. A Laboratory Manual”,


Fourth edition, 1996, Addison Wesley Publishes.


Reference:


2

P.Gunasekaran et.al. “L
aboratory Manual in Microbiology” , 1995, New Age
International (P) limited publishers.

3.

Kannan. N, 1995. Laboratory manual in General Microbiology, Palani paramount
publications, Palani.