# B.TECH BIOTECH II YR II SEM

Biotechnology

Feb 12, 2013 (5 years and 5 months ago)

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BIOTECHNOLOGY SYLLABUS

SCHEME OF INSTRUCTION AND EXAMINATION

B.Tech. (Biotech)
-

II YEAR
-
II Semester

SUBJ.CODE

SUBJECTS

PERIODS /
WEEK

The /Lab

MARKS

DURATION OF EXAM.

Hrs

Sessional

Exams

Hours

BTT.2.201

Mathematics

11

4

30

70

3

BTT.2.202

Microbiology

4

30

70

3

BTT.2.203

Genetics and Molecular
Biology

4

30

70

3

BTT.2.204

Electrical and
Electronics Engineering

4

30

70

3

BTT.2.205

Instrumental Methods
in Biotechnology

4

30

70

3

BTT.2.206

Industrial
Biotechnology

4

30

70

3

BTP.2.20
7

Microbiology Lab

4

25

50

4

BTP.2.208

Electrical & Electronics
Engineering Lab.

4

25

50

4

BTP.2.209

Instrumentation Lab.

4

25

50

4

355

570

MATHEMATICS
-

II

Subject code :
BTT.2.201

Sessional : 30

Periods / Week : 4

Examination : 70

Nature of Examination : Theory

Exam Duration : 3 Hrs

UNIT
-

I.

Complex numbers, curves and regions in complex plane. Function of a complex variable. Limit,
continuity, Derivative of function of complex variable. Analytic funct
ions. Harmonic Function,
Cauchy
-

Reimann equations.

UNIT
-

II.

Mapping by elementary functions, conformal mapping, Mapping of the functions

W = Z
z
, W = e
z
, W = 1/z, W = Z +l/z, W = Sin Z; and W = Cos
z.
Bilinear transformation.

UNIT
-

III.

Line integra
l in the complex plane. Basic properties of the complex line integral. Cauchy's
theorem, Cauchy's integral formulae. Evaluation of line integral.

UNIT
-

IV.

Taylor's theorem. Taylor's series of elementary functions. Laurent's theorem, Laurent's

expa
nsion of functions. Analyticity at infinity, zeroes and singularities.

UNIT
-

V.

Revidues
-

calculation of residues. Integration by the method of residues.

Contou integration (J
2
^ / ( Cos 0, Sin 6 ) d 0 ; J / (x)dx)
O
-
00

EXAMINATION :

One q
uestion from each unit with internal choice.

TEXT BOOKS:

1. Higher Engineering Mathematics, by B.S. GAREWAL, Khanna Publishers ( (1998)

2. Advanced engineering Mathematics (Fifth edition) Wiley Eastern, Erwin Kreysizig,

MICROBIO
LOGY

Subject code :
BTT.2.202

Sessional : 30

Periods / Week : 4

Examination : 70

Nature of Examination : Theory

Exam Duration : 3 Hrs

UNIT
-

I: HISTORY AND INTRODUCTION TO MICROBIOLOGY

Historical Developments in Microbia
l; Biochemical tests
-
Sugar fermentations, IMVIC tests,
Catalase production etc.Contributions of Antony Van Leiwenhoek, Louis Pasteur, Robert Koch,
Iwanowskii, Edward Jenner; Outline of historical development in microbial biotechnology
applications (Food, P
harma, Environmental and industrial applications) Identification of
Microorganisms: Staining techniques; Principle dyes, Fixation, simple staining, differential
staining, spore staining and flagella staining.

UNIT
-

II MAJOR GROUPS OF MICROORGANISMS

Ge
neral and Colony characters of major groups of Microorganisms
-

Algae, Fungi, Protozoa,
Bacteria; Identification of Microorganisms by Major Taxonomical Characteristics
(Morphological, Physiological, Ecological, Cultural, Metabolic/Biochemical, Immunologic
al
and Genetic). Classification of Microorganisms
-

Concept of Classification; Taxonomic Groups;
Haeckel's three kingdom concept, Whittaker's five kingdom concept, Three domain concept of
Carl Woose.

UNIT
-

III: VIRUSES AND THEIR CHARACTERS

Genera! Chara
cters of Viruses and Life cycles (Lytic and Lysogenic); Structure and Replication
of Bacteriophage (T2) and Lamda; Tobacco Mosaic Virus (TMV), Retrovirus, HIV and Prions;
Classification of Viruses
-

Major Characteristics for Classification, Characteristi
cs of each group;
Methods of Cultivation of Viruses; importance of Viruses in Biotechnology.

UNIT
-

IV: MICROBIOLOGICAL TECHNIQUES

Methods of Culturing of Microorganisms in Lab and Industry
-

Culture media, (Liquid, Semi
-
solid and Solid media, Synthetic

media and Complex media), Types of media (simple, routine
lab, selective, differential, enrichment and enriched), Isolation of pure cultures (Streak, Spread
and pour plate methods). Concept of Sterilization
-

Methods and Their Application
-

Physical
Method
s (Heat, Filtration and Radiation), Chemical methods (Phenolics, Alcohols, Halogens,
Heavy metals, Dyes, Quaternary Ammonium compounds,, Aldehydes, Gaseous agents);
Methods of Preservation of Microorganisms and their importance; Maintenance and preservatio
n
of bacterial cultures.

UNIT
-
V: MICROBIAL PHYSIOLOGY AND GROWTH

Nutrition in Microorganisms and Assimilation of Major Nutrients; Nutritional groups of
microbes and their importance in fermentation Industry; Microbiological Media and Their
Application Gro
wth of Microorganisms
-

Growth factors, Growth curve, Mathematical
expression of growth, Measurement of microbial growth (Cell numbers and Cell mass),
Importance of Growth Phases of Microorganisms; Balanced and unbalanced growth, factors
affecting bacteria
l growth (Solutes, Water activity, pH, temperature, oxygen concentration,

EXAMINATION
: One question from each unit with internal choice

TEXT BOOKS:

1.

Microbiology, 5
th

edition, Pelczar, Michael J, Krieg, Noel R.; Jr.; Chan, E. C,
McGrawHill. (1993).

2.

Textbook Of Microbiology by Dubey Rc, Maheshwari Dk S Chand & Company Ltd.
(2006).

3.

Industrial Microbiology
-

Prescott and Dunn.4
th

edition, CBS Publ.& Distributors.

REFERENC
E BOOKS:

1.

Prescott L.M. Harley J.P Klein D.A Microbiology,6 th ed, (2004), McGraHill

Brooks G.F, Butel J.S., Morse S.A., Medical microbiology, 23
rd

ed McGraHill (2004).

2.

Johnson A.G., Ziegler. R.J Microbiology and Immunology,4 th ed , ((2002)), Lippincott
W
illiams & Wilkins publishers, Baltimore.

3.

Microbiology, 5
th

edition, Pelczar, Michael J, Krieg, Noel R.; Jr.; Chan, E. C, McGraw
-
Hill.

4.

Principles of Microbiology, Atlas, RM, 2
nd

edition, W.M.C. Brown Publishers,
Dubuque,(1996)

5.

Microbiology: An Introduction by Gerard J. Tortora, Berdell R. Funke, Christine L.
Case
. Pub: Benjamin Cummings; 10
th

edition (2009).

6.

Alcamo's Fundamentals of Microbiology, Effrey C. Pommerville 8th Edition Jones &
Bartlett Publishers; 8th edition (2006).

7.

Benson's Microbiological Applications: Laboratory Manual in General Microbiolog
y,
Short Version by Alfred Brown McGraw
-
Hill Science/Engineering/Math; 11
th

edition
(2008)

8.

Microbiology by Prescott/Harley/Klein's Joanne Willey, Linda Sherwood , Chris
Woolverton, McGraw
-
Hill Science/Engineering/Math; 7
th

edition, (2007).

GENETICS AND
MOLECULAR BIOLOGY

Subject code :
BTT.2.203

Sessional : 30

Periods / Week : 4

Examination : 70

Nature of Examination : Theory

Exam Duration : 3 Hrs

UNIT
-
I:
PHYSICAL BASIS OF HE
REDITY

Baisc laws of inheritance, mono, di,

tri, hybrid ratios, Mendel’s laws, Linkage and crossing over:
cytological basis, Identification of genetic materials: classical experiments by Griffith, Avery,
Mc Cleod, Hershy Chase etc. RNA as genetic material, pseudo genes, overlapping and split
gene
s.

UNIT
-
II: GENETIC RECOMBINATION, MAPPING & MUTATIONS

Transformation, conjugation, F
-
mediated sexduction, generalized, specialized transduction,
mechanism of recombination & recombination frequencies; Mapping of bacterial chromosomes
and construction

of genetic map
of E.coli
Mutations: Spontaneous, induced, lethal mutagens,
their types & actions, classification of mutations, characters of mutations and applications.
Maryleon's hypothesis, sex linked disorders in human beings.

UNIT
-
III: DNA STRUCTURE,

REPLICATION & REPAIR

Structure of DNA
-
Watson & Crick's model; Types of DNA
-

A
-
DNA, B
-
DNA, Z
-
DNA;
Denaturation and renaturation of DNA. Replication of DNA
-

Semi conservative replication,
enzymology of replication, continuous and discontinuous DNA synthesis
, complex replication
apparatus, unidirectional replication, bi
-
directional replication, rolling circle replication; DNA
damage and repair: Types of DNA damages
-

deamination, alkylation, pyrimidine dimmers;
Repair mechanisms
-
Excision repair and mismatch re
pair, SOS repair,
rec
gene and its role in
DNA repair.

UNIT
-
IV: MECHANISM OF TRANSCRIPTION

Structure of Promoters
-

RNA Polymerases of Prokaryotic and Eukaryotic Organism;
Transcription
-

Initiation, Elongation and Termination; Post Transcriptional Processe
s of
Eukaryotic RNA; Structural features of RNA
-

rRNA, mRNA, tRNA and functions of RNA.
Transcription apparatus and proteins involved in transcription. Prokaryotic & Eukaryotic
transcription. Processing of t
-
RNA, r
-
RNA, m
-
RNA splicing; Concept of Ribozyme,

Inhibitors
of Transcription.

UNIT
-
V: MECHANISM OF TRANSLATION

Ribosome
-

Structural features of prokaryotic and eukaryotic ribosome; Genetic code
-
Triplet
code, cracking of genetic code, features of genetic code, wobble hypothesis; Protein synthesis:
Trans
lation in prokaryotes and eukaryotes
-

initiation of translation, elongation of polypeptide
chain, termination of translation. Post translation modification, protein transport, Inhibitors of
Protein Synthesis.

EXAMINATION
: One question from each unit
with internal choice

TEXT BOOKS:

1.

Principles of Genetics. Gardner, E. J., Simmons, M. J., Snustad, D. P. and Snustad,
(1985). John Wiley and Sons, Inc.

2.

Genetics
-
BD Singh 3
rd

edition. Kalyani Publications

3.

Cell and Molecular Biology: Concepts and Experim
ents by Gerald Karp. Wiley; 4
th

Edition (2004).

4.

Molecular Biology
-
Friefelder, 2
nd

edition Narasa Publ, (2007).

REFERENCE BOOKS:

1.

Principles of Genetics
-
Robert H.Tamarin, Tata McGraw Hill 6th edition (1998).

2.

Molecular Biology of the Gene, Fifth Edition b
y James D. Watson , Tania A. Baker,
Stephen P. Bell, Alexander Gann , Michael Levine , Richard Losick ,. Benjamin
Cummings; 5
th

edition (2003).

3.

Molecular cell biology

Lodish H., Berk A, Matsudaira P., Kaiser CA., Krieger M.,
Scott MP., Zipurski SL.,
and Darnell J5
th

ed. WH Freeman & Co. (2003).

4.

Genetics and Molecular Biology by Dr. Robert F. Schleif. The Johns Hopkins University
Press; 2
nd

edition, (1993).

5.

Molecular Biology of the Cell by Bruce Alberts , Alexander Johnson , Julian Lewis ,
Martin Raff

, Keith Roberts, Peter Walter. Garland Science; 5
th

edition

6.

Genetics and Molecular Biology by Dr. Robert F. Schley, The Johns Hopkins University
Press; 2
nd

edition, (1993).

7.

Schaum's Outline of Genetics by Susan Elrod, McGraw
-
Hill; 4 Editions, (2001).

8.

Ge
netics: Analysis of Genes and Genomes by Daniel L. Hartl, Elizabeth W. Jones.

Jones
& Bartlett Publishers; 6
th

Edition, (2004).

9.

Real
-
Time PCR: Current Technology and Applications. Julie Logan, Kirstin Edwards and
Nick Saunders Academic Press. (2009).

10.

Molec
ular Biology of the Cell By Maurice Wilkins Publisher: Garland Science (2002).

ELECTRICAL AND ELECTRONICS ENGINEERING

Subject code :
BTT.2.204

Sessional : 30

Periods / Week : 4

Examination : 70

Nature of Examination : Theor
y

Exam Duration : 3 Hrs

UNIT
-

I.

D.C. Circuits and network theorems: Kirchoff s laws
-

mesh current and node voltage analysis
-

superposition theorem
-

thervenin's theorem
-

Norton's theorem
-

maximum power transfer
theorem.

UNIT
-

II.

Electroma
gneticInduction: Electromagnetic Induction
-

faraday's laws of electromagnetic
induction
-
Direction of Induced e m f and current
-

Induced e m f self inductance
-

Mathal
inductance
-

coupling co
-
efficient Rise of current in an Inductive circuit
-

decay of
current in an
inductive circuit
-

energy stored in a magnetic field
-

Hysteresis and eddy current losses. D.C
Generators generator principle action of commentator construction of D.C. Generator
-

types of
armature winding E.M.F. equation
-

types of generat
ors
-

losses and efficiency
-

armature
reaction
-
characteristics of different types of generators

UNIT
-

III.

D.C Motors: working principle
-

Back E M F voltage and power equation
-

Types of D.C.
Motors torque and speed of a D.C motor
-

losses and efficien
cy characteristics of different types
of D.C. Motors
-

speed control of D.C. shunt and series motors shunt motor starter.

A.C. Circuits: Generation of alternating voltages and currents
-

equation of alternating voltage
and current average and r m s value o
f sinusoidal quantities
-

form and peak factor
-

pharos
representation of sinusoidal quantities
-

addition and subtraction of alternating quantities
-

A.C.
circuit containing resistance only Inductance only
-

capacitance only
-
R
-
LR
-
C,R
-
L~C, series
circuits

-

parallel A.C circuits.

UNIT
-
IV.

Single phase transformer: Constructional details
-

working principle of an Ideal transformer
-

E
M F equation
-

practical transformer on no load and on load
-

equivalent circuit
-

voltage
regulation
-

losses and effic
iency
-

condition for minimum efficiency
-

open circuit and short
circuit test. Three phase Induction Motors: advantages and Disadvantages
-

Construction
-

Principle of production of rotating magnetic field
-

working principle of Induction motor
-

slip
rot
or current frequency effects of slip on the rotor circuit
-

rotor current starting torque condition
for minimum starting torque
-

induction motor under load
-

torque under running conditions
-

-

Torque
-

slip characteristics
-
Losses and Ef
ficiency.

UNIT
-
V.

Electronic Devices & Circuits:
-

P
-

n Junction
-

Semiconductor diode
-

characteristics of diode

Diode as rectifier
-

Half wave
-

Full wave rectifier
-

Bridge rectifier
-

Filter circuits. Transistor
-

Construction
-

Transistor action Tr
ansistor symbols
-

Transistor as an amplifier

common base
connection of a transistor
-

characteristics of common base connection
-

common emitter
connection of a transistor
-

characteristics of common emitter connection transistor as an
amplifier in C E
arrangement.

EXAMINATION:
One question from each unit with internal choice

TEXT BOOKS:

1.

Principles of Electrical engineering and Electronics by V.K. Mehta. S.Chand & Co.
(1996) Edn.

2.

Basic Electrical And Electronics Engineering (2007) by R.k. Rajput Pu
blisher: Laxmi
Publications

REFERENCE BOOKS:

1.

Electrical Technology, B.L. Theraja & A.K. Theraja, S Chand & Co.

2.

Teach Yourself Electricity and Electronics, Fourth Edition by Stan Gibilisco McGraw
-
Hill/TAB Electronics; 4 edition, (2006).

3.

Electronic and El
ectrical Engineering: Principles and Practice by L.A.A. Warnes.

Palgrave Macmillan; 3Rev Ed edition, (2002).

4.

Bird, J.O. Electrical and Electronic Engineering Principles. Longman 1987

5.

Boylestad, Robert L. Introductory Circuit Analysis. Prentice
-
Hall (2003)
10th Edition.

6.

Digital Electronics: Principles, Devices and Applications.Anil Kumar Maini, Wily
Eastern. (2007).

7.

Industrial Electricity and Motor Controls by Rex Miller, Mark Miller,

McGraw
-
Hill
Professional; 1
st

Edition (2007).

INSTRUMENTAL METHODS IN
BIOTECHNOLOGY

Subject code :
BTT.2.205

Sessional : 30

Periods / Week : 4

Examination : 70

Nature of Examination : Theory

Exam Duration : 3 Hrs

UNIT
-
I: ANALYTICAL METHODS

Types of Analytical Methods
-

Instruments for Anal
ysis
-

Uncertainties in Instrumental
measurements
-

Sensitivity and detection limit for instruments.

UNIT
-
II: INSTRUMENTS FOR ISOLATION TECHNIQUES

Cell disruption by French press, Sonification, freeze thaw technique; use of liquid N
2

and
chemical approach
es involved in cell disruption; Isolation of Biomolecules and cell organelles:
centrifugation; basic principles of sedimentation, sedimentation coefficient, Svedberg Unit;
various types of centrifuges, their uses, rotors, fixed angle, vertical, swing out,
zonal rotors;
preparative centrifugation, differential density gradient centrifugation, analytical ultra
centrifugation; Materials used in preparation of density gradient
-

sucrose & cesium chloride;
Isolation of sub cellular organelles and Biomolecules. De
termination of molecular weight and
purity of Biomolecules by analytical ultra centrifugation.

UNIT
-
III: SEPARATION TECHNIQUES

Partition coefficient, partition chromatography, counter current distribution, adsorption
chromatography, Paper, TLC& GLC, adsor
ption media, solvent, continuous and gradient elution,
fraction collection and detection of pure molecules. Methods based on size: Gel permeation
chromatography, principle application
-

Molecular weight determination. Dialysis and its
significance. Affinity

chromatography, application & technique for purification of proteins and
nucleic acids.

UNIT
-
IV: CHARGE BASED SEPARATION TECHNIQUES

Principle and application of Ion exchange chromatography, use of ion exchange
-

cation & anion
exchangers, pH and salt grad
ients for elution of proteins, amino acids and nucleotides.
Electrophoresis: Migration of charged molecules in electric field
-
moving boundary, paper,
cellulose acetate, starch gel electrophoresis, SDS PAGE, Determination molecular weight, iso
-
electric focu
sing and its significance. Identification of specific proteins by western blotting.
Agarose gel electrophoresis
-

separation of DNA & RNA, by agarose gel electrophoresis,
recovery of DNA fragments from agarose gels, southern & northern blot techniques and
their
significance, pulse field gel electrophoresis.

UNIT
-

V: SPECTROMETRIC IDENTIFICATION TECHNIQUES

Basic concepts of spectroscopy, Visible & UV spectroscopy & Explain Beer lamberts law;
Principles and application of Colorimetry & Flame photometry, Neph
lometry; Principles and
applications of Atomic absorption Spectrophotometry; Principles & applications of IR, ESR
NMR & Mass spectroscopy; Explains the laws of photometry.

Examination:

One question from each unit with internal choice.

EXAMINATION:
One q
uestion from each unit with internal choice

TEXT BOOKS:

1.

Fundamentals of Analytical Chemistry
-

8
th

edi. Skoog DA, West DM and others
Thomson Brooks/Cole Eastern Press Bangalore, (2005).

2.

Instrumental Methods of Chemical Analysis
-

GW Ewing 4
th

edition Mc Gr
aw Hill.
(1985).

3.

Principles and Techniques of Biochemistry and Molecular Biology

by Keith Wilson , John Walker. 6
th

edition Cambridge University Press. (2005).

REFERENCE BOOKS:

1.

Readings in Scientific American, W.H.Freeman, (1985)
-
(1993).

2.

Instr
umental Methods for Quality Assurance in Foods (Food science & technology) by
Daniel Y.C. Fung (Editor), Richard F. Matthews

CRC Press; 1
st

Edition 1991.

3.

Basic Laboratory Methods for Biotechnology, 2/E Lisa A. Seidman, Madison

Cynthia J. Moore, Publish
er: Benjamin Cummings, (2009).

4.

Separation processes in the food and biotechnology industries By A. S. Grandison,

5.

Near
-
infrared applications in biotechnology By Ramesh Raghavachari , CRC Press,
(2001).

6.

N
atural products isolation By Richard J. P. Cannell , Published by Humana Press, (1998).

7.

Biotechnology and Fermentation Process. By Mr. John I. D'souza, Mr. S. G. Killedar
Published by Pragati Books Pvt. Ltd., (2008)

8.

Analytical biotechnology By Thomas G. M.

(2002).

9.

Chromatography: concepts and contrasts By James M. Miller Edition: 2, Published by
John Wiley and Sons, (2005).

10.

Thin layer chromatography in chiral separations and analysis By Teresa Kowalska,
Joseph Sherma,

CRC Press, (2007)

11.

Microscopic techniques in biotechnology By Frank Mayer, Michael Hoppert

-
VCH, (2003).

12.

Agricultural biotechnology By A. Altman, Rita R. Colwell Published by CRC Press,
1997

13.

Animal cell biotechnology: methods and
protocols. By Ralf Pörtner Edition: 2,

14.

Food analysis by HPLC By Leo M. L. Nollet Edition: 2, Published by CRC Press,
(2000).

15.

Modern instrumental analysis By Satinder Ahuja, Neil D. Jespersen Published by
Else
vier, (2006).

16.

Fundamentals of contemporary mass spectrometry, By Chhabil Dass Published by Wiley
-
Inter Science, (2007).

INDUSTRIAL BIOTECHNOLOGY

Subject code :
BTT.2.206

Sessional : 30

Periods / Week : 4

Examination : 70

Na
ture of Examination : Theory

Exam Duration : 3 Hrs

UNIT
-
I: INTRODUCTION TO BASICS OF BIOTECHNOLOGY

A historical overview on scope and development of biotechnology and products; Biotechnology
as an interdisciplinary enterprise; A brief survey of orga
nisms, processes, products and market
economics relating to modern industrial biotechnology; Concepts of tools and techniques used in
biotechnology; Areas of application of biotechnology.

UNIT
-
II: INTRODUCTION TO INDUSTRIAL BIOPROCESSES

Role of a bioproce
ss engineer in the Biotechnology Industry; Introduction, Development and
Maintenance and characterization of Industrial Microorganisms; Primary and Secondary
Screening of Innoculum, starter and industrial cultures, Analysis of microbial fermentation
proce
sses; Batch and Continuous fermentations, process controls, oxygen supply and demand,
single and multiple bubble aeration, sparger aeration, foam control equipment, scale
-
up of
Fermentors; An overview of aerobic and anaerobic fermentation processes.

UNIT
III: PRODUCTION OF PRIMARY AND SECONDARY METABOLITES

A brief outline of processes for the production of some commercially important Organic acids
(e.g. Citric acid and Lactic acid); Amino acids (Glutamic acid and Lysine); Alcohols (Ethanol,
and n
-
Butanol).

Study of Production processes for various classes of low molecular weight
secondary metabolites
-
" Antibiotics, Beta
-
lactams (Penicillins), Amino glycosides
(Streptomycin), Macrolides (Erythromycin), Quinones, Aromatics and Vitamins (B12).

UNIT
-
IV: PRODUC
T DEVELOPMENT

P
roduction of beverages (Beer,wine), Production of commercially important industrial enzymes,
Production of recombinant proteins : insulin interleukins, Tumor necrosis factor.

UNIT
-

V: SPECIALITY BIOPRODUCTS FOR AGRICULTURAL, FOOD AND
P
HARMACEUTICAL INDUSTRIES

Bio
-
pesticides; Bio
-
fertilizers and Plant growth factors; Natural Biopreservatives (Nisin);
Biopolymers (Xanthan gum and PHB); Single cell protein; High fructose corn syrup;
Bioconversion of vegetable oils; Production

of semi
-
synthetic penicillins and cephalosporins;
Racemically pure drug intermediates; Steroid bioconversions.

EXAMINATION
: One question from each unit with internal choice

TEXT BOOKS:

1.

Biotechnology: Text Book of Industrial microbiology
-

CruegerW & C
ruegerA.

2
nd

edition,
Panima Pub, (2005).

2.

Industrial Microbiology, LE Casida.

1
st

edition,
New Age Int, (2006).

3.

Industrial Microbiology, AH Patel. Mc Millan India ltd., (2007).

4.

Industrial Biotechnology Author: R. Singh, S.k. Ghosh Publishing: (2004).

Pub: Global Vision.

REFERENCE BOOKS:

1.

Industrial Microbiology
-

Prescott and Dunn.4
th

edition, CBS Publ.& Distributors.

2.

Microbial Biotechnology: Fundamentals of Applied Microbiology
-
Glazer AN and Nikaido.
2
nd

edition, Cambridge University Press
.

3.

Principles of Fermentation Technology
-

Stanbury PF, A Whitaker
&.
SJ Hall 2
nd

edition
Elesvier Science Ltd.

4.

Organic Chemistry of Natural Products
-
OP Agarwal Vol I & II Goel Publishing House.

5.

Industrial Biotechnology P.R. Yadav, (2005) Pub. Discovery

Publishing House

6.

Industrial Biotechnology Varun Shastri : (2006) Publisher: Isha Books

7.

Industrial Enzymes and Their Applications by Helmut Uhlig, Wiley
-
Interscience (1998).

8.

Industrial Biotechnology by Shara L. Aranoff, Robert A. Rogowsky, Karen Lane
y
-
Cummings, Deanna Tanner Okun, Daniel R. Pearson, Nova Science Publishers, Incorporated,
Pub. (2009).

9.

Industrial Biotechnology Shara L Aranoff; Daniel R Pearson; Deanna Tanner Okun; Irving
A Williamson; Dean A Pinkert; Robert A Rogowsky; Karen Laney
-
Cumm
ings,Pub: Nova
Science Publishers, Inc. (2009).

10.

Industrial Pharmaceutical Biotechnology Klefenz, Heinrich, Wiley
-
VCH ((2002).

MICROBIOLOGY LABORATORY

Subject code :
BTP.2.207

Sessional : 25

Periods / Week : 4

Examination

: 50

Nature of Examination : Practicals

Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

(Minimum of 8 Experiments in the list are to be performed)

1. Microscopic Observation, Staining and Identification of Bateria, Fungi, Algae' and Protozoa
-

Identi
fication of microorganism:

(a) Simple & Differential staining techniques (b) Biochemical testing

2. Calibration of Microscope & Measurement of Microorganisms
-

Microtomey.

3. Sterilization techniques (Autoclaving, Hot Air Oven, Radiation and Filtration)

4.

Preparation of culture media (a) broth type of media (b) Agar

5. Culturing of microorganism (a) broth (b) pure culture techniques
-

Streak, Pour plate, flask,
Isolation and preservation of bacterial culture.

6. Antibiotic tests
-

Disc diffusion method, mi
nimum inhibitory concentration.

7. Biochemical tests
-

IMIVC test, Catalase, Coagulase test, Gelatinase test, Oxidase.

8. Factors affecting the bacterial growth and study of growth curve

9. Measurement of Microbial Growth (Viable Count and Turbidometry)

10. Hanging drop technique

11. Colony counters
-

Enumeration of Bacterial numbers by serial dilution

12. Identification of animal and plant bacterial cells

13. Bacteriological Analysis of Foods

14. Yeast and Mould count in foods

BOOKS:

1.

1. Laboratory Expe
riments in Microbiology by M Gopal Reddy
et al

Himalaya
Publishing House PVT Ltd.

2.

Laboratory manual in Microbiology
-
P Gunasekaran, New Age International Publ., New
Delhi.

3.

Laboratory manual in General Microbiology NKannan ., Panima Publ. Corp., New Delhi.

ELECTRICAL & ELECTRONICS ENGINEERING LAB.

Subject code :
BTP.2.208

Sessional : 25

Periods / Week : 4

Examination : 50

Nature of Examination : Practicals

Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

(Minimum of 8 Experiments in the list is to be performed)

1.

Verification of Theremins & Norton’s theorems

2.

Verification of Maximum Power Transfer theorem & superposition theorem

3.

Power factor measurement of and R
-

L series circuit

4.

Calibration of single phas
e Energy meter

5.

Magnetization Characteristics of separately excited generator

6.

Load characteristics of shunt generator

7.

Swin burnes Test on D.C shunt Motor

8.

Seed Control of D.C Shunt Motor

9.

Brake Test on D.C Shunt Motor

10.

Brake Test on Induction motor

11.

Open
-
circui
t & short circuit Tests on single phase transformer

12.

Static Characteristics of junction diode

13.

Static characteristics of a common base & common emitter transistor circuit.

14.

Brake test on D.C Series motor

15.

Three
-

phase power measurement by two watt meter met
hod

16.

Regulation of single phase alternator

INSTRUMENTAL METHODS OF ANALYSIS LABORATORY

Subject code :
BTP.2.209

Sessional : 25

Periods / Week : 4

Examination : 50

Nature of Examination : Practicals

Exam Duration : 4 Hrs

LIST OF EXPERIMENTS

(Minimum of 8 Experiments in the list are to be performed)

1.

The calibration of pH meter and measurement of pH for different solutions.

2.

Estimation of Ascorbic acid by colorimetric assay.

3.

Estimation of unknown samples by using conduct
ivity meter.

4.

Estimation of different macromolecules by visible spectrophotometer.

5.

Verification of Lambert
-

Beers law by UV
-
VIS spectrophotometer.

6.

Estimation of proteins
&
nucleic acids by U.V method.

7.

Estimation of turbidity using Nephlometer.

8.

The separation of different macromolecules by Paper, Thin layer chromatography.

9.

The separation of different macromolecules by Paper, PAGE, SDS
-
PAGE.

10.

Estimation of minerals by Flame photometry.

11.

Estimation of Thiamine and Riboflavin by Fluorimetry.

12.

Preparati
on of Standard curve using UV
-
VIS & Flame Photometry

13. Fractionation of Plasma Proteins by Electrophoresis.

14. Biosensors.

Books:

1. Modern Experimental Biochemistry 3
rd

Edition
-

Boyer R, Pearson Education, (2000).

2. Instrumental Methods of Chemical

Analysis (8
th

Edition)
-

Sharma B.K, Gel Publishing
House, ((1999)).