bt 2257 microbiology lab mr. n. sathish

crewbullBiotechnology

Feb 12, 2013 (4 years and 6 months ago)

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DOC/LP/01/28.02.02


LESSON PLAN

LP
-

BT 2257


LP Rev. No:
03

Date:
26
/12/
2012

Page 1 of 2


Sub Code & Name :
BT 2257

MICROBIOLOGY LAB

Branch:
BIOTECHNOLOGY


Semester :
IV


MAIN AIM(S) OF THE C
OURSE

The course aims to develop the skills of students in area of microbiology. Here hands on
training is offered for the students to study microbes, their identifications & characterization an
d
their practical uses.


LIST OF EXPERIMENTS

Expt. No

Name of the Experiments

1

Laboratory safety and sterilization techniques

2

Microscopic methods in the identification microorganisms

3

Preparation of culture media
-

nutrient broth and nutrient agar

4

Culturing of micro organisms
-


in broth and in plates (pour plates, streak plates,
isolation and preservation of bacterial cultures)

5

Staining Techniques
-

Grams’ and differential

6

Quantification of microorganisms

7

Effect of disinfectants on microbi
al flora

8

Isolation and identification of micro organisms from different sources
--

soil, water
and milk

9

Isolation and identification of microorganism
s from clinical samples

(Extra)

10

Isolation and identification of

fungi from contaminated food

(Ex
tra)

11

Antibiotic sensitivity assay

12

Growth curve

observation and growth characteristics of bacteria and yeast

13

Effect of different parameters on bacterial growth (pH, Temperature, UV radiation)

14

Screening of microorganism producing industrial
enzymes

(Extra)

15

Model lab







DOC/LP/01/28.02.02


LESSON PLAN

LP
-

BT 2257


LP Rev. No:
03

Date:
26
/12/
2012

Page 2 of 2


Sub Code & Name :

161451

MICROBIOLOGY LAB

Branch:
BIOTECHNOLOGY


Semester :
IV


Week

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15

Units

1

2

3

4

5

6

7

8

9

10

11

12

13

14

15


REFERENCES:


1.

Micro Biology : Laboratory Theory and applications, M.J. Heboffee aw BE Pierce

Morten
Publishing House, 2006.

2.

Mackie

and Mccartney, practical medical microbiology, 14
th

Edition, Internationalstudent
edition, Churchill living stone 1996




Prepared by

Approved by




Name

Mr. N. Sathish

Prof.
Sulochana somasundaam

Designation



Asst. professor



HOD
-
Biotechnology

Date

26/12/2012

26/12/2012