Molecular Biotechnology Group


Feb 12, 2013 (4 years and 4 months ago)


Molecular Biotechnology Group
刘相梅 Xiang-mei LIU, Professor
Address: State Key Laboratory of Microbial Technology
School of Life Science, North Building
Shandong University
Shanda Nan Road 27#
Jinan, 250100, Shandong Province
P. R. China
Phone: +86-531-88365992
Fax: +86-531-88565610
2005-present Professor , State Key Lab of Microbial Technology, Shandong University
2000-2005 Associate Professor, State Key Lab of Microbial Technology, Shandong University
1996-2001 Ph.D. in Microbiology, Shandong University
1992-1993 Visiting Scholar in University of Sydney, N.S.W. 2006, Australia
1984-1991 M.Sc. and B.Sc. in Microbiology, Shandong University
1. Microbiology on extremely acidophilic bacteria in biomining
2. Genetic methods and genetic modification in biomining bacteria
3. Metabolism of organic substances by obligately, chemolithoautotrophic Acidithiobacilli
4. Molecular mechanisms of alkaline or alkali-tolerant cellulase and xylanase

The colonies on solid sodium thiosulfate media and cells
of Acidithobacillus caldus MTH-04 stained with
erythrosin under microscopy
The colonies on solid FeTSB double layer plate
and cells of Leptospirillum ferriphilum under
scanning eletron microscopy
TCA cycle showing the
deficient enzyme in
Acidithobacillus sp.
The 3D structures of xylanase
constructed by homology
1. Xiangmei Liu*, Jianqun Lin, Keli Tian, and Wangming Yan. Metabolism of organic compounds by extremely acidophilic, obligately
chemolithoautotrophic Thiobacilli: a review. Chineses J. Biotechnol., 2008, 24(1):1-7
2. Y Qin, X Wei, X Liu, T Wang, Y Qu*. Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in
Saccharomyces cerevisiae with higher glycosylation and stability. Protein expression and purification, 2008, 58:162-167
3. X Liu*, J Lin, Z Zhang, J Bian, Q Zhao, Y Liu, J Lin, and W Yan. Construction of conjugative gene transfer system between E. coli and moderately
thermophilic, extremely acidophilic Acidithiobacillus caldus MTH-04. J. Microbiol. Biotechnol., 2007, 17(1):162-167
4. Liu Xiangmei, Hu Yuhong, Kong Jian, Wu Zhihong*, Gimmicky commentation on the cultivation character and metabolism mechanism of different
strains in the transformation experiment, Microbiology, 2006, 33(3): 173-176
5. Ting Wang, Xiangmei Liu*, Qian Yu, Xi Zhang, Yinbo Qu, Peiji Gao, Tianhong Wang. Directed evolution for engineering pH profile of
endoglucanase III from Trichoderma reesei, Biomolecular Engineering, 2005, 22/1-3: 89-94
6. Zhao Qing, Liu Xiangmei*, Zhan Yang, Lin Jianqun, Yan Wangming, Construction of an engineered Acidithiobacillus caldus with high-efficiency
arsenic resistance, Acta Microbiologica Sinica, 2005, 45(5): 675-679
7. Zhao Qing, Liu Xiangmei*, Bian Jiang, Zhan Yang, Liu Ying, Lin Jianqun, Yan Wangming, Construction of the arsenic resistance plasmids and the
conjugative transfer to Thiobacillus caldus, Biotechnology, 2005, 15(3): 19-21
8. K Tian, J Lin, X Liu, Expression of E. coli phosphofructokinase gene in an autotrophic bacterium Acidithiobacillus thiooxidans. J. Microbiol.
Biotechnol.,2004, 14(1): 56-61
9. Liu Xiang-mei, Yi-bo Qu, Fan You, Ying Liu, Studies on the key amino acid residues responsible for alkali-tolerance of the xylanase by site-directed
or random mutagenesis. Journal of Molecular Catalysis B: Enzymatic, 2002, 18: 307-313
10. Liu Xiang-mei, Meng Qi, Jian-qiang Lin, Zhi-hong Wu, Yi-bo Qu, Asparagine residue at position 71 is responsible for alkali-tolerance of the
xylanase from Bacillus pumilus A-30. Journal of Microbiology and Biotechnology, 2001, 11(3): 534-538