M.Sc. DEGREE EXAMINATION 2012

clusteriranianBiotechnology

Oct 23, 2013 (3 years and 11 months ago)

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Total No. of Pages:

2

Register Number:







62
1
1


Name of the Candidate:


M
.
Sc
.

DEGREE
EXAMINATION


20
1
2


(
BOTANY
)


(
SECOND
YEAR)


(
PAPER
-
V
I
I
)

2
3
0
.
BIOTECHNOLOGY, MOLEC
ULAR BIOLOGY AND GEN
ETIC
ENG
INEERING

December
)

Maximum: 100 Marks





(
Time: 3 Hours

SECTION
-
A





Answer
ALL
Questions



(
8
×
3
=
2
4
)

All questions carry equal marks

Define the following:

1.


Cosmid

2.


Single cell protein

3.


Hup gene

4.


Topoisomerase

5.


Introns

6.


Housekeeping genes

7.


Electroporation

8.


T
-
DNA

SECTION
-
B






Answer
ALL
Questions



(
6
×
6
=
36
)


All questions carry equal marks

9.


a)

Explain briefly the processes of blunt end ligation

(OR)

b)

Write an account on Restriction Endonuclease

enzymes.

10.


a)

Explain briefly DNA finger printing technique.

(OR)

b)

Describe briefly about the organisation of Nif genes.

11.


a)

Explain the structure of compound gene.

(OR)

b)

Write an account on DNA super coiling.

12.


a)

Explain the functions of RNA polymerases in transc
ription.

(OR)

b)

Describe the Components of Operon.

13.


a)

Write an account on C
-
value paradox

(OR)

b)

Explain the role of Biotechnology in Health and Environment hazards.




2

14.


a)

Write an account on Ti plasmid

(OR)

b)

Write short notes on viral vectors

SECTION
-
C






Answ
er ALL Questions



(2×20=40)


All questions carry equal marks

15.


a)

Describe the principle and applications of PCR

(OR)

b)

Write a detailed note on (i) Chromatin structure (ii) Agrobacterium mediated gene transfer

16.


a)

Describe the
process of Transcription in Prokar
yotes

(OR)

b)

Write an essay on Transgenic plants.

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