Detailed Bio-data of the Course Coordinator (2) - IASRI

clattergumneckBiotechnology

Oct 23, 2013 (3 years and 7 months ago)

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1


Proforma for submitting proposal for organization of Training Programmes
under Centre of Advanced Faculty Training (CAFT) in frontier and
specialized areas of agriculture and allied sciences (2013
-
2014)



1.

Topic of the Training Programme

:


“Advanced ‘
Omics


Techniques for Improvements


in Plant and Human H
ealth

.


2.

Justification of the proposed programme in the light of suggestive training needs in
the discipline
(not more than 100 words
)


One of the most pressing challenges for the next 50 years would
be to reduce mortality
occurring worldwide due to chronic and non
-
communicable diseases such as diabe
tes,
obesity and cancer. Plant s
cience could contribute significantly to addressing this Grand
Challenge

by understanding the complex relationships between

diet and health

through
identification of metabolites

(p
hytonutrients)

that promote human health and reduce the
risk of chronic diseases

and
by
deciphering the underlying mechanisms

that help to
prevent or ameliorate these diseases
.

Metabolic engineering
of plants
could

then be
deploy
ed

to enhance the concentrati
o
n

of these phytonutrients

in crop plants to

desired
levels

for deriving

optimal benefits.

These bio
-
fortified, nutritionally
-
rich plants would
not only be beneficial for human health but would be more tolerant to biotic and abiotic
stresses, themselves.

However, to accomplish these objectives

trained human resource

in
advanced “omic” technique
s

would be required to work in this emerging field of plant
science i.e. functional foods and nutragenomics.

Unfortunately, human resource required
to carry out this
onerous
task is inadequate at present and there are still many training
gaps in the
capabilities of the available manpower in this field. In our pursuit to achieve
this objective, many basic training programmes have been organized at our centre in the
past
. Over a period of time, the Division of Biochemistry has acquired the requisite
fac
ilities and infrastructure to carry out the work in the field of metabolic engineering,
encompassing all ‘Omic’ techniques. Currently, the Division is
ambitiously pursuing the
research in this field.

The
proposed training programme would
,

therefore,
be an
attempt
to expose the teachers/resea
rchers from ICAR and SAUs in this area for their

better
understanding of plant and human health.


3.

Venue with full postal/e
-
mail address and office phone/fax numbers

Division of Biochemistry, Indian Agricultural Research
Institute, New Delhi
-
110012
Tel: 011
-
25842038; E
-
mail:
head_biochem@iari.res.in

4.

Proposed dates:

Nov. 12
-

Dec.

0
2
, 2013
-

(21 days)


2



5.

Eligibility qualification for the participants of the
Training programme


i.

Master’s Degree in Plant Biochemistry/ Agril. Chemistry, Plant Molecular
Biology and Biotechnology and related disciplines.


and

ii.

Working not below the rank of Assistant Professor and equivalent in the
above mentioned subject under
Agricultural University/I.C.A.R Institute


6.

Information regarding proposed Course Coordinator,
if other than the Director
,
CAFT,

(enclose bio
-
data clearly bringing out the specific qualification, experience and scientific
contribution of the Course Coordina
tor in the proposed topic
):


1)

Dr. Anil Dahuja, Course Coordinator





-

Annexure I

2)

Dr. Archana Singh, Course Coordinator





-

Annexure II


7.

Faculty Staff strength in CAFT
(
Assistant Professors, Associate Professors, Professors and
equivalent
)

i.

Dr. R.D. Rai,
Principal Scientist & Head

ii.

Dr. Archana Sachdev, Principal Scientist

iii.

Dr. Aruna Tyagi, Principal Scientist

iv.

Dr. Shelly Praveen, Senior Scientist

v.

Dr. Anil Dahuja, Senior Scientist

vi.

Dr. Archana Singh, Senior Scientist

vii.

Dr. Dharam Paul Chaudhary, Senior Scientist

viii.

Dr. Ranjit Ranjan Kumar, Scientist

ix.

Dr. Suneha Upadhaya, Scientist

x.

Dr. Vinutha T., Scientist

xi.

Ms Veda Krishnan, Scientist


8.

Information regarding other academic staff of the host Institute who are likely to be
used as resource persons:




Dr. N.K. Singh,
National Professor, NRCPB, New Delhi



Dr. Srinivasan, Professor & PS, NRCPB, New Delhi



Dr. A.K. Saxena, Head & PS, Division of Microbiology, IARI, New Delhi.



Dr. Suresh Wallia, Professo
r & PS, Division of Agri. Chem.
, IARI, New Delhi



Dr. T.R. Sharma, Princi
pal Scientist, NRCPB, New Delhi.



Dr. Charanjit Kaur, Principal Scientist, PHT Division, IARI, New Delhi



Dr. C. Vishwanathan,

Principal Scientist, Plant Physiology,

IARI,

New Delhi




3


9.

Specific facilities available for conduc
ting the Programme such as Laboratory
equipments/instruments, research farm, library, classroom, guesthouse etc.


i.

Real Time PCR

ii.

Gel Documentation System

iii.

HPLC

iv.

GLC

v.

High
-
Speed low temperature Centrifuges and Microfuges

vi.

Scintillation counter

vii.

Thermo cyclers

viii.

Tissue Culture Laboratory


10.

Programmes/Projects and achievements in the area of special topic proposed for the
training programme








Annexure

III


11.


Tentative schedule of daily lectures/practical topics to be covered and name of the
faculty proposed to b
e engaged during CAFT Training Programme:


S. No.

Date / Day

Topic of lecture/Practical

Name and Designation of
Speaker

1

12
-
11
-
2013
,
Tuesday

Biochemistry and Agricultural
Biotehnology


Immobilized enzymes


tool for
development of nanoprobes and
biosensors


( L)


Isolation of Plant DNA
-
(P)

Dr. S. L. Mehta, Former VC,
MPUAT


Dr.
Arvind M. Kayastha
,

Professor, BHU


Dr. Ranjeet Ranjan Kumar,

Scientist

2

13
-
11
-
2013
,
Wednesday

An overview of Plant Genomics (L)



Restriction analysis

of DNA
-

(P)

Dr.
N.K. Singh, National
Professor


Dr. S.

Goswami,

Scientist

3

14
-
11
-
2013
,
Thursday

Transgenic Plants
-

Concepts and
applications

( L)


Southern Blot analysis of plant DNA
-

(P)

Dr. Srinivasan,
Director(Acting), NRCPB


Dr. Ranjeet Ranjan Kumar,
Scientist

4

15
-
11
-
2013
,
Friday

Role of small RNAs in abiotic stress
tolerance

( L)


RNA isolation and its expression analysis
using RT
-
PCR

Dr. C.S. Vishwanathan
,
Principal Scientist
, IARI



Dr. Archana Sachdev and Dr.
Veda

Krishnan, Scientist

5

16
-
11
-
2013
,
Saturday

Event characterization and integration of
transgenics in

Plant breeding

( L)


RNA isolation and its expression analysis
using RT
-
PCR
-

(P)

Dr. A.K. Singh, Principal
Scientist
, IARI


Dr. Archana Sachdev and Dr.
V.

Krishnan, Scientist

4


6

18
-
11
-
2013
,
Monday

Modulation of

catalytic activities of
Ubiquitin
-
proteasome system during biotic
stress


( L)

Quantitative expression analysis of RNA
by Real Time PCR
-

(P)

Dr. Shelly Parveen, Principal
Scientist
, IARI



Dr. Archana Singh, Senior
Scientist

7

19
-
11
-
2013
,
Tuesday

Metagenomics

( L)


G
ene Cloning and Transformation
.
-

(P)

Dr. A.K. Saxena, Principal Sci.
& Head
, IARI

Dr. Aruna Tyagi, Principal
Scientist

8

20
-
11
-
2013
,

Wednesday

Stress Memory in Plants

(L)


Gene Cloning and Transformation.
Contd……
(P)

Dr. Archana Singh, Senior
Scientist

Dr. A
runa

Tyagi, Principal
Scientist

9

21
-
11
-
2013
,

Thursday

Protein/Enzyme Engineering

(
L)



Bimolecular

Fluorescence
complementation (BiFc) technique
-

(P)

Dr. Anil Dahuja, Senior
Scientist


Dr. Shelly Parveen,
Principal
Scientist

10

22
-
11
-
2
013
,

Friday

Enhancing nutritional traits of functional
foods through enzymatic processing and
nutraceutical incorporation

( L)


Plasmid D
NA isolation and Protein
Profiling


Dr. Charanjit Kaur, Principal
Scientist



Dr.
Archana Singh, Senior
Scientist

11

24
-
11
-
2013
,

Saturday

Nutrigenomics
-
(L)


2
-
D Gel electrophoresis and isoelectric
focussing.
-

(P
)

Dr. Archana Sachdev, Principal
Scientist

Dr. Veda


Krishnan, Scientist

12

25
-
11
-
2013
,

Monday


Metabolomics

( L)


2
-
D Gel electrophoresis and isoelectric
focussing.
-

(P)
-

Contd
….

Western Blotting.
-

(P)

Dr. R.D. Rai, Principal Scientist
& Head

Dr.
V.

Krishnan, Scientist

13

26
-
11
-
2013
,
Tuesday

Functional food qualities of natural
food colors

(L)


Metabolite
profiling using HPLC and
GC
-
MS.
-

(P)

Dr. Suresh Wallia, Professor &
PS




Dr. Anil Dahuja, Senior
Scientist

Dr
. Vinutha T., Scientist

14

27
-
11
-
2013
,
Wednesday

Metabolic Pathway Engineering

Potential
and Challenges

( L)


Metabolite profiling

using HPLC and GC
-
MS.
-

(P)
-

Contd
……….

Dr. M. L. Lodha, Emeritus
Scientist(ICAR)



Dr. Anil Dahuja, Senior
Scientist

Dr. Vinutha T., Scientist

15

28
-
11
-
2013
,
Thursday

Transcription factors and gene
expression: role and regulation
-
(L)


MALDI
-
TOF and
Microarray.
-

(P)

Dr. Aruna Tyagi, Principal
Scientist


Dr.
N. K. Singh, National
Professor, ICAR

5


16

29
-
11
-
2013
,
Friday

Bioinformatics tools for plant genome
analysis

(L)



Hands
-
on Bioinformatics tools.
-

(P)



Next

Generation Sequencing
techniques


(P)

Dr. T.R. Sharma, Principal
Scientist
, NRCPB


Dr. T.R. Sharma, Principal
Scientist
, NRCPB


Dr. Kishor Gaikwad, Senior
Scientist, NRCPB

17

30
-
11
-
2013

Saturday

Emerging Challenges for Biochemistry and
Biotechnology in
Agri
-
industries.


Amino Acid Profiling using UPLC
-

(P)

Dr. G. K. Garg



Dr. D.P

Cha
udhary, Senior
Scientist, DMR


18

02
-
12
-
2013

Open House Interaction

&

Valedictory

Function

Participants & Faculty
Members, Divn. of
Biochemistry,



12.

Name of the Trainings
organized during the last three years.


i.

Current Biochemical and Molecular Techniques for Nutritional Enhancement and
Stress Tolerance in crop Plants (November 01
-
21, 2012)

ii.

Current Techniques and Protocols in Plant Biochemistry and Molecular Biology
(
8
th

December, 2011 to 28
th

December, 2011)

iii.

Advanced Techniques in Plant Biochemistry and Molecular Biology

(February 18
-

March 10, 2011)




13.

Signature of the Director of the CAFT
:

_____________________________________

(with official seal)








6


Annexure 1

Detailed Bio
-
data of the Course Coordinator (1)

1.

Name




:

Dr. ANIL DAHUJA

2.

Designation



:

Senior Scientist

3.

Specialization



:

Plant Biochemistry & Biotechnology

4.

Institution’s Address


:

Division of Biochemistry







Indian Agricultural Researc
h Inst.







Pusa Campus, New Delhi
-
12

5.

Residential Address


:

EG
-
2, S/F, Inderpuri, New Delhi
-
12

6.

Date of Birth



:

13
th

August 1973

7.

Contact Details


:



Telephone


:

011
-
25842038 (O)







011
-
25834374 (R)







91
-
9818379701 (M)



E
-
mail



:

ad_bio@yahoo.com







anilda@iari.res.in







dahuja.anil@gmail.com

8.

Academic Qualifications

:

S.
No.

Degree

University/

Instit
ute


Specialization

Year of
Passing

Thesis Title

1.

Ph.D

(Biochem.)

Kurukshetra
University,
Kurukshetra

Biochemistry/

Biotechnology

2009

“Cloning and Characterization of
the Gene Encoding Ribosome
Inactivating /Antiviral protein
from leaves of
Bougainvillea
xbuttiana.


2.

M.Sc

(Biochem.)

Kurukshetra
University,
Kurukshetra

Biochemistry/

Biotechnology

1995


“Without thesis”

3.

B.Sc

Punjab
University,
Chandigarh

Chemistry

Botany

Zoology

1993


“Not applicable”



7


9.

List of Publications


:

(I)

Recent
Research Papers
:


Dubey A, Sharma Dew, Srivastav M., Singh
A., Pandey R., Dahuja Anil (2012
) Effect of
paclobutrazol and putrescine on antioxidant enzymes activities and nutrients content in
salt
-
tolerant citrus rootstock sour orange under sodium chloride stress.
Journal of Plant
nutrition
(Accepted).

Singh O.P., Usha K., Saboki E., Srivastav
Manish, Dahuja Anil

and Singh B. (2012
)
Enzymatic reactive oxygen species (ROS) scavenging system in mango varieties resistant
and susceptible to malformation.
Scientia Horticulturae

138: 81
-
89
.

Swati Sharma, Sharma RR, Pal R
K, Paul Vijay, Dahuja Anil (201
2
) 1
-
methylcyclopropene influences biochemical attributes and fruit softening enzymes of
“Santa Rosa” Japanese plum (
Prunus salicina

Lindl
.) J. Plant Biochem

Biotechnol

21(2):295
-
299.

Jhalagar, MJ, Sharma RR, Pal, RK, Arora, A and Dahuja Anil. (2011)
Analysis of
physiological and biochemical changes in Kiwifruit (
Actinitia dealicious

cv. Allison)
after post harvest treatments with 1
-
methyl cyclopropene (1
-
MCP).
J. Plant Biochem
Biotechnol

20 (2): 205
-
210.

Patel SK, Dubey A K, Srivastav M, Singh AK,
Da
huja A
and Pandey R. N. (2011)
Effect of NaCl in the irrigation water on growth, anti
-
oxidant enzyme activities, and
nutrient uptake in five citrus rootstocks.

Journal of Horticultural Science &
Biotechnology

86
(2) 189

195

Dew Kumari Sharma, Dubey AK, S
rivastav Manish, Singh Ak, Sairam RK, Pandey RN,
Dahuja Anil & Kaur Charanjit (2011) Effect of putresine and paclobutrazol on growth,
physiochemical parameters and nutrient acquisition of salt
-
sensitive citrus rootstock
Karna Khatta

(
Citrus Karna
Raf.) und
er NaCl stress.
J. Plant Growth Regulation
. 30 (3):
301
-
311.

Manish Srivastav, Arun Kishor

and Anil Dahuja (2010) Effect of paclobutrazol and
salinity on ion leakage, proline content and activities of antioxidant enzymes in mango
(
Mangifera indica

L.).

Scientia Horticulture
125(4): 785
-
788.

Kishore Arun, Srivastava Manish, Dubey AK, Singh AK, Anil Dahuja and Pandey RN
(2009) Paclobutrazol minimizes some negative effects of salt stress in mango.
Journal of
Horticulture Science and Biotechnology
84(4) 459
-
465
.

Charanjit Kaur, Kiran Kumar, Anil Dahuja and Kapoor HC (2007) Variation in
antioxidant activity in Broccilli (
Brassica oleracea
L) cultivars.
Journal of Food
Biochemistry

31(5): 621
-
638.

8


R.R. Sharma, Hare Krishna, V.B. Patel, Anil Dahuja and Rajbi
r Singh (2006) Fruit
calcium content and lipoxygenase activity in relation to albinism disorder in strawberry.
Scientia Horticulture
, 107: 150
-
154.

Anil Dahuja and Madaan TR. (2004) Off
-
flavour development in soybeans: Comparative
role of some antioxidants

and related enzymes.
Journal of Science of Food and
Agriculture

84: 547
-
550.

Anil Dahuja and Madaan TR. (2003) Estimation of Parameters responsible for the
generation of off
-
flavour in some Indian varieties of soybean.
Plant Foods for Human
Nutrition

58(3
): 1
-
8.


Anil Dahuja and Madaan TR. (2000) Relationship between isocitrate lyase activity in
germinating seedlings and chilling sensitivity.
Indian J. plant Physiol., Vol.5, No.2, (N.S)
pp 205
-
207.

(II)


Book
-
Chapters
:



Anil Dahuja and Gupta VK (2010)

Antiviral/Ribosome Inactivating Proteins for
strengthening plants’ defense against viruses.
In:

Genetic Engineering
-

A new hope for
crop production and improvement (Ed. C.P. Malik) Aavishkar Publishers, distributors,
Jaipur, pp 202
-
233.

(III)

Manuals
Prepared
:


Dahuja Anil, Madaan TR, Johari RP and Lodha ML (January, 2002).. A Laboratory
Manual on Plant Biochemistry, Division of Biochemistry, IARI, New Delhi
-
110012

Prikhshayat Singh and Anil Dahuja (March 2007). A Laboratory Manual on Basic
Biochemi
stry, Division of Biochemistry, IARI, New Delhi
-
110012

Kumar RR, Dahuja Anil, Goswami S ,
Santha IM, Rai RD(2013) A Laboratory Manual
on Inorganic Nitrogen Metabolism, Division of Biochemistry, IARI, New Delhi
-
110012

Anil Dahuja, Archana Singh, I. M. Santha, R. D. Rai (2012). A Centre of Advanced
Faculty Training Manual on
Current biochemical and molecular techniques for nutritional
enhancement and stress tolerance in crop plants, Division of Biochemistry, IARI, New
De
lhi
-
110012

10.

Patent List, if any:


A full
-
length cDNA sequence of 1168 bp length, encoding an antiviral protein from
leaves of
Bougainvillea

x
buttiana

has been submitted to NCBI GenBank with
Accession
Number EU682417
.






9


11.

List of Projects
implemented:



Cloning and characterization of genes encoding antiviral proteins of non
-
host plants.



Isolation and characterization of differentially regulated genes under moisture stress
in rice.



Development of superior varieties and hybrids for higher nutr
ition, value addition and
export.



Biochemical studies on the biogenesis of off
-
flavour in soybean.



Cloning and characterization of the gene encoding Ribosome inactivating/Antiviral
protein from leaves of
Bougainvillea

x
buttiana
.


Details of Research Proje
cts being implemented/completed/submitted


S.
No.

Project Title

Project
Status

Duration/

Date of Start

Funding
agency



1.

Elimination of off
-
flavour
component of soybean by the use
of biochemical and molecular
tools.


Ongoing


April 2009
onwards


In
-
house
project


2.

Isolation of Gm phytase/MIPS
gene for engineering its
expression in soybean seeds.


Ongoing


April 2009
onwards


In
-
house
project

3.

Use of RNAi technology in
developing low phytate soybean
and rice.

Ongoing

June 2011

onwards

NFBSFARA,
ICAR
Project


4.

Identification of dwarf and salt
tolerant rootstock(s) in mango and
citrus.


Ongoing


April 2009
onwards


In
-
house
project

4.

Cloning and characterization of
genes encoding antiviral proteins
of non
-
host plants.


Completed


2004
-
2009


In
-
house
project

5.

Biochemical studies on the
biogenesis of off
-
flavour in
soybean.


Completed


1999
-
2003


In
-
house
project

6.

Development of superior varieties
and hybrids for higher nutrition,
value addition and export.


Completed


2004
-
2009


In
-
house
project

7.

Isolation and characterization of
differentially regulated genes
under moisture stress in rice.



Completed


2004
-
2009


In
-
house
project



10


Annexure

II


Detailed Bio
-
data of the Course Coordinator (2)


Name


:

Dr. (Mrs) Archana Singh






Senior Scientist (Biochemistry)

Date of Birth :

19
th

June, 1964

Address


:

Flat no. 663, Plot no. C 58/3


Ushma Urja Society, Sector
-

62


NOIDA 201 301, U.P., INDI
A

Contact nos. : 91
-

120
-

2401159 (Resd.)


Cell no.: 9810424146




E
-
mail :
sarchana19@yahoo.com











sarchana19@gmail.com


Official address



: Room No.406, III
-
Floor, Biochemistry Division,


IARI, New Delhi
-
12

Academic Qualifications:

Degree

Board/University

Year

%
Marks

Div

Remarks

High School

U.P. Board

1980

68.2

I

Distinction in
Maths and Science

Intermediate

U.P. Board

1982

68.4

I

Distinction in
Biology

B.Sc. (ZBC)

Lucknow
University

1984

77.1

I

2
nd

Position in
ZBC group

M.Sc. (Biochemistry)

Lucknow
University

1986

70.1

I

3
rd

Position in
order of merit

Proficiency in
German Language

Lucknow
University

1987




Ph.D. (Biochemistry)

Lucknow
University

1992




Research Associate
-
ship (CSIR)

Lucknow
University

1994
-
1999




Research Associate
-
ship (DBT)

IIRGSP &
IITGSP, NRCPB,
IARI, New Delhi

2000
-
January
06,2010




11



1.
Research
Associateship
: Project entitled
-

“Indian Initiative on Tomato Genome
Sequencing”

(2005
-
January06,

2010,

NRCPB,

New Delhi)

2
. Research Associateship
: Project entitled
-


Indian Initiative on Rice Genome Sequencing”

(2000
-
05, NRCPB, New Delhi)


3.
Research Associateship
: Project entitled
-

“Molecular,Cytological and Biochemical Aspects
of Parasitization by
Cuscuta

(1994
-
99, Luck
now University,Lucknow)

Ph.D. Thesis Title

“Studies on the Mechanism of Parasitization by
Cuscuta



(1987
-
92, Lucknow University,Lucknow)

Other Qualif
ications:

Papers Published


:
Seventeen

(National /International)

Electronic Data


: Total 19,000 Accessions of sequences for crops
-

Rice, Tomato,
Wheat, Mustard, Pigeon Pea, Brinjal and Sugarcane at

GenBank
site (NCBI).

Papers Presented in



: Thirty

Two
(National/International)

Seminars / Symposia

Specialization

: Plant Biochemistry (Enzymology & Physiology), Plant Molecular
Biology and Plant Biotechnology (Mapping, Cloning, Sequencing,
Genoinformatics), Construction of Genomic & cDNA
libraries to
generate Genetic Molecular Markers for Crops’ Development &
Improvement.

Research Experience

: Twenty Years

Techniques Known:

Enzymology

: Protein purification, enzyme assays, Gel electrophoresis (native
and SDS
-
PAGE), Microtomming.

Plant Tis
sue Culture

: Media Preparation, root and shoot organ cultures, dual cultures,
biological hardening.

Molecular Biology

: Extraction and purification of DNA, Polymerase Chain
Reaction(PCR), DNA elution from gel, Plasmid DNA isolation,
Mapping, Cloning and S
equencing of BAC/PAC clones of
specified region of chromosomes during Genomic library
Construction and its Applications, Construction of cDNA library to
develop Molecular markers, Isolation of RNA, RT
-
PCR,
12


Quantitative (Real
-
time) PCR, Microarray technique
s, Agarose gel
electrophoresis, Gel Documentation and Finishing strategies
(Transposone Tagging, Assembly and Annotation).

Bioinformatic Tools
BLAST X & BLAST P, Gene prediction (FGENESH, GENESCAN,
GENEMARK), Phrap, Phred and Consed, Primer Desi
ging
-

primer 3, SSR IT, Clustal W.

Computer knowledge:


MS Word, MS Excel, MS Power Point, Photoscanning (Adobe
Photoshop), Web Browsing.

Achievements

Fellowships:



Junior Research Fellowship for two years
(1986


88)
under Special Assistance
Programme of

University Grants Commission, Govt. of India.



Senior Research Fellowship for three years
(1988


91)

under Special Assistance
Programme of University Grants Commission, Govt. of India.



Research Associateship for five years
(1994
-

99)

under Council for Sc
ientific &
Industrial Research (CSIR), Ministry of Science & Technology, Govt. of India.



Research Associateship in DBT funded International Project “Indian initiative on Rice
Genome Sequencing Project” (IIRGSP) at NRC on Plant Biotechnology, Indian
Agricu
ltural Research Institute (IARI), New Delhi (
2000
-
05).



Research Associateship in DBT funded International Project “Indian initiative on Tomato
Genome Sequencing project (IITGSP) at NRC on Plant Biotechnology, Indian
Agricultural Research Institute (IARI),
New Delhi (Since July 2005
).


Awards / Prizes



Ph.D. Awarded entitled “Studies on the Mechanism of Parasitization by
Cuscuta
” from
University of Lucknow, Lucknow, India

(1992).



Awarded Research Associateship under Council of Scientific and Industrial Research
(CSIR), Ministry of Science and Technology, Govt. of India
(1994


99).



Availed Young Scientist Award from IUBMB (International Union of Biochemistry and
Molecular Biology)
in
1994.



Best Poster Award at the “International symposium on Rice: from Green Revolution to
Gene Revolution” held at Hyderabad, India (2004).



Letter of Appreciation by Indian Prime Minister
-

Shri Atal Bihari Vajpayee to
Consortium members of IIRGSP (2005)
.



Letter of Appreciation for being “First Indian scientists’ group” to sequence Arhar
Genome byDG
-

ICAR, Ministry of Agriculture, Govt. of India (2012).

13



Research Projects Handled

Type of project

Details such as Title,
PI/Co
-
PI, Allocation,
etc.

Duration

Accomplishments

PROJECT 1

Sponsored

Molecular, cytological
and biochemical
aspects of
parasitization by
Cuscuta.

(As PI

CSIR funded
project )

5 years

(i) Differential variation in photosynthetic
pigments and carotenoides in shoot (
Cuscuta
)
and
root (
Orobanche
) parasites along the
length of Haustoria were reported (ii)High
activity of cell wall degrading enzymes in
haustoria of parasites, and, protein synthesis
at 80s cytoplasmic ribosomes as a prerequisite
for haustorial formation was reported f
or the
first time (iii)Anatomical and enzymatic
studies on susceptible and resistant hosts of
Cuscuta.
revealed defence responses in the
hosts and parasites.

PROJECT 2

Contactual

Indian Initiative on
Rice Genome
Sequencing project


( Associate
-
DBT
funded p
roject))

Four years &
Eight months

(i) 7MB of long arm of chromosome 11 of
rice (Oryza sativa var Japonica)was
sequenced and submitted toGenBank

(ii) Comparative analysis of rice and wheat
genomes was carried and highly conserved
synteny of single copy gen
es of rice with
wheat was reported..

(iii)A map based rice gene database was
generated

PROJECT 3

Contractual

Indian Initiative on
Tomato Genome
Sequencing project
(Associate
-
DBT
funded project))

Three years &
Seven months

(i) 2.2 MB of short arm of
chromosome 5 of
tomato (Lycopersicon esculentum var Heinz
1706) was sequenced and submitted to
GenBank

PROJECT 4

(In
-
house)

Biochemical and
molecular studies on
abiotic stresses in
cereals.(Associate
-
ICAR
-
funded project)

2009
-
2014

Running…….





14


List o
f
Publications:


A.

Recent
Research Papers:

1)

Nagendra K. Singh, Deepak K. Gupta, Pawan K. Jayaswal, Ajay K. Mahato, Sutapa
Dutta, Sangeeta Singh, Shefali Bhutani, Vivek Dogra, Bikram P. Singh, Giriraj
Kumawat, Jitendra K. Pal, Awadhesh Pandit, Archana
Singh, Hukum Rawal, Akhilesh
Kumar,G. Rama Prashat, Ambika Khare, Rekha Yadav, Ranjit S. Raje, Mahendra N.
Singh, Subhojit Datta, Bashasab Fakrudin, Keshav B. Wanjari, Rekha Kansal, Prasanta
K. Dash, Pradeep K. Jain, Ramcharan Bhattacharya, Kishor Gaikwad
& Trilochan
Mohapatra & R. Srinivasan &Tilak R. Sharma. The first draft of the pigeonpea genome
sequence. (2012).

Journal of Plant Biochemistry & Biotechnology, 21(1): 98
-
112.

2)

Himabindu Kudapa
,
Arvind K. Bharti
,
Steven B. Cannon
,
Andrew D. Farmer
,
Benjamin

Mulaosmanovic
,
Robin Kramer
,
Abhishek Bohra
,
Nathan T. Weeks
,
John A. Crow
,
Reetu Tuteja
,
Trushar Shah
,
Sutapa Dutta
,
Deepak K. Gupta
,
Archana Singh
,
Kishor
Gaikwad
,
Tilak R. Sharma
,
Gregory D. May
,
Nagendra K. Singh

and
Rajeev K.
Varshney.
A
Comprehensive Transcriptome Assembly of Pigeonpea (
Cajanus cajan

L.)
using Sanger and Second
-

Generation Sequencing Platforms: Molecular Plant (2012), 1
-
9.

3)

Archana Singh as one of the member of Consortium
-

Indian initiative on Tomato
Genome Sequencing Proj
ect (IITGSP)
.
The tomato genome sequence provides insights
into fleshy fruit evolution.

Nature (2012), 485:635

641.

4)

Archana Singh as one of the member of Consortium
-

Indian initiative on Tomato
Genome Sequencing Project (IITGSP), (2009). A Snapshot of the E
merging Tomato
Genome Sequence: Tomato Genome Sequencing Consortium. The Plant Genome, 2
(1):78
-
92.

5)

B.K.Singh, Archana Singh, K.Gaikwad and N. K. Singh (2009). Cloning and
Characterization of full
-

length triticum cDNA and Genes from Wheat Varieties K
-
68
and Chinese spring. Journal of Plant Biochemistry & Biotechnology, 18(1):21
-
28.

6)

Swarup K. Parida,
S. Kaul, S.K. Kalia, V. Dalal, G. Hemaprabha, A. Selvi, A. Pandit, A.
Singh, K. Gaikwad, T.R. Sharma, P.S. Srivastava, N.K. Singh and T. Mohapatra (2009).
Informative genomic microsatellite markers for efficient genotyping applications in
sugarcane.
Theore
tical and Applied Genetetics.

118:327
-

338.

7)

V. Jyothilakshmi, A. Singh, K. Gaikwad, K. Vinod, N. K. Singh and S.M.S. Tomar
(2008). RNA Editing in CMS wheat: Influence of nuclear background leads to
differential editing on
orf
256. Indian Journal of Geneti
cs and Plant Breeding. 68(4):353


359.

8)

Nagendra K. Singh, Vivek Dalal, Kamlesh Batra, Binay K. Singh, G. Chitra,
Archana
Singh
,
Irfan A. Ghazi, Mahavir Yadav, Awadhesh Pandit, Rekha Dixit, Pradeep K.
Singh, Harvinder Singh, Kirpa R. Koundal, Kishor Gaikw
ad, Trilochan Mohapatra, Tilak
R. Sharma
.
(2007
). Single copy genes define a conserved order between rice and wheat
for understanding differences caused by deletion, duplication and transposition of genes.

Funcunal and Integrative Genomics. 7:17
-
35 (19).

9)

T
.R. Sharma, M.S. Madhav, B.K. Singh, P. Shanker, T.K. Jana, V. Dalal, A. Pandit,
A.
Singh
, K. Gaikwad, H.C. Upreti and N.K. Singh
(2005).

High resolution mapping,
15


cloning and molecular characterization of the PiKh gene of rice, which confers resistance
to
M. grisea.

Molecular Genetetics & Genomics: 274 (6): 569
-
578.

10)

The rice chromosomes 11 and 12 sequencing consortia.

(2005).
The sequence of rice
chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications.

).

BMC Biology

3:20
-

37.

11)

IRGSP
:

(2005)
. The map based sequence of the rice genome.

Nature
.
436:793
-
800.

12)

Nagendra K. Singh, Saurabh Raghuvanshi, Subodh K. Srivastava, Anupama Gaur, Ajit
K. Pal, Vivek Dalal,
Archana Singh
, Irfan A. Ghazi, Ashutosh Bhargav, Mahavir Yadav,
Anupam Dixi
t, Kamlesh Batra, Kishor Gaikwad, Tilak R. Sharma, Amitabh Mohanty,
Arvind K. Bharti, Anita Kapur, Vikrant Gupta, Dibyendu Kumar, Shubha Vij, Ravi
Vydianathan, Parul Khurana, Sulabha Sharma, W. Richard McCombie, Joachim
Messing, Rod Wing, Takuji Sasaki, Pa
ramjit Khurana, Trilochan Mohapatra, Jitendra P.
Khurana and Akhilesh K. Tyagi.
(2004)
. Sequence analysis of the long arm of rice
chromosome 11 for rice
-
wheat synteny.

Functional and Integrative Genomics 4:102
-
117.


B
.
Electronic Publications/ Patents



(A
rchana Singh as one of the authors)


~97000 ESTs/Patents from R
ice, Tomato, Wheat, Brinjal, Mustard and Pigeonpea.

C.
Manual

Prepared:



Anil Dahuja, Archana Singh, I. M. Santha, R. D. Rai (2012). A Centre of Advanced
Faculty Training Manual on Current
biochemical and molecular techniques for nutritional
enhancement and stress tolerance in crop plants, Division of Biochemistry, IARI, New
Delhi
-
110012

………………………………………………………………………………………………………











16


Annexure III

In
-
house Projects

Project
-
1

:Biochemical and Molecular Studies on the Abiotic Stresses in


Cereals.

Sub
-
Projects :





Sub
-
Project 1.1: Biochemical and molecular basis of thermo
-
tolerance in wheat (
Triticum
aestivum L.
).

Sub
-
Project 1.2: Isolation and characterization of

regulatory genes associated with water
deficit stress in rice.


Sub
-
Project 1.3: Biochemical and molecular studies on high affinity potassium
transporter (HKT) of wheat (
Triticum aestivum L.
) under

salinity stress.

Project 2:

Biochemical and molecular characterization of nutritionally
significant principles of soybean


Sub
-
Projects:


Sub
-
Project 2
.1
:

Isolation of Gm phytase/MIPS gene for engineering its expression
in soybean seeds.

Sub
-
Project

2.2:

Elimination of off
-
flavour from soybean by biochemical and
molecular tools.

ICAR
-
funded Projects

i.

Use of RNAi technology in developing low phytate soybean and rice.
(NFBSFARA Project)

ii.

National initiative on Climate Resilient Agriculture (NICRA)

Achie
vements in the proposed area:



Major genes involved in fatty acid and triacylglycerol biosynthetic pathways were
isolated and characterized with the aim to uncover and understand their role oil quality
and quantity.



The microsomal ω
-
6 desaturase gene (
fad
2
-
1
) was isolated from the developing soybean
seeds and characterized for soy oil quality improvement. Three gene silencing constructs
-
sense, antisense and intron spliced hairpin were successfully designed and transformed
into model plant
Arabidopsis

to check their efficacy
.



With a view to reducing the phytate content in soybean, gene
s

encoding
phytase

and
MIPS

has been isolated from soybean and expressed in
E.coli
.

17




A binary vector pBIN
-
MIPShp (14kb) harbouring MIPS
-
RNAi expression cassette,
driven
under the control of seed specific promoter, was constructed through three times
sub cloning via mid clone vector and successfully transformed into soybean
via

Agrobacterium

mediated transformation protocol. The positive primary transformants
were verif
ied by PCR screening primed by gene specific primers and transgenic plant
selection marker primers.



An AP2/ERF transcription factor encoding gene associated with drought stress has been
isolated, cloned, sequenced and re
-
cloned in a binary vector (PBI121).

Recombinant
plasmid has been mobilized in
Agrobacterium

and transferred to
Arabidopsis

and rice.



Antiviral/ribosome inactivating proteins were isolated and characterized from the leaves
of
Chenopodium, Amaranthus, Celosia and Bougainvillea.

Genes encodin
g these proteins
were expressed in
E.coli

and corresponding proteins obtained in
E.coli
.




Role of lipid and protein oxidation has been studied in the off
-
flavour generation in
soybean and methods devised to ameliorate its flavor quality.



Role of Heat Shock

Proteins

(HSPs) was studied in thermo
-
tolerance in wheat through
gene expression analysis.



Proteomic characterization of wheat cultivars has been carried out under differential heat
stress using 2D
-
PAGE and differentially expressed proteins identified, wh
ich are
predicted either to be HSPs or antioxidant enzymes.

………………………………………………………………………………………………