BIOTECHNOLOGY & GENETIC ENGINEERING - Ohio University

clattergumneckBiotechnology

Oct 23, 2013 (3 years and 7 months ago)

112 views


1

PBIO 450
0
/550
0
:
BIOTECHNOLOGY & GENETIC ENGINEERING

(3

credits)


Time & Place:

Tuesday & Thursday
12:00
-
1:2
0

PM
; Porter
104


Instructor:

Allan Showalter

Fall 20
1
3


Text:

Molecular Biotechnology (4
th

Edition)

©2010 by Bernard R. Glick,
Jack J. Pa
sternak

a
nd
Cheryl L. Patten


Course web

site
:

http://www.ohio.edu/plantbio/staff/showalte/PBIO%20450%20&%20550/


Prerequisites
:

PBIO1140

or BIOS 170
0


Course Description:

The purpo
se of this course is to introduce students to basic molecular biological concepts and
techniques used in the fields of biotechnology and genetic engineering
.
Current experimentation and
progress in these fields as well as ethical considerations of this res
earch will be discussed.


Learning Objectives:



Gain

an appreciation and knowledge of how to deal with ethical issues relating to science
.



Learn

how

to conduct a comprehensive literature review on a biological topic.



Learn

how to read and understand primary

publications in the scientific literature.



Learn

how to present scientific research data to an audience.



Gain

an u
nderstanding of basic molecular and cellular biology concepts and techniques.



Gain

an u
nderstanding of current experimentation in biotechnolo
gy
and

genetic engineering.


Grades will be based on the following:



1.

A midterm exam on the first third of the course (100 points).


2.

A midterm exam on the second third of the course (100 points).


3.

A comprehensive final exam (100 points).


4.

A gra
ded, in
-
class presentation on a biotechnology or genetic engineering topic, preferably a
controversial one, to be approved by the instructor (50 points for PBIO 450 students and 25
points for PBIO 550 students)
.
Each student will have to prepare a one
-
para
graph abstract of
his or her presentation and a list of relevant references (
including the article titles
) for distribution
to the class
.
Graduate students enrolled in the course will additionally have to submit an 8
-
10
page double spaced paper (25 points)

on their chosen topic following the format of a review
paper
in
Nature Reviews Molecular Cell Biology

(
http://www.nature.com/nrm/index.html
),

being sure to include
complete article titles for all refere
nces.


5.

Homework

(50
points). E
mail answers to
assigned homework

questions
to
showalte@ohio.edu

as a MS Word attachment file
. Chapter answer sets are given equal grade weighting and

are
due each Monday by noon

for

the past week’s assignments.


Thus,
there are 400 points possible
.

Typically, 93.3% and above will earn an A, 90
-
93.3% an A
-
, 86.7
-
90% a B+, 83.3
-
86.7% a B, 80
-
83.3% a B
-
, 76.7
-
80% a C+, 73.3
-
76.7% a C, 70
-
73.3% a C
-
,
66.7
-
70% a D+, 63.3
-
66.7% a D, 60
-
63.
3% a D
-
, and below 60% an F.


E
xams will be based upon material covered in class lectures as well as in the assigned readings.



2

Office Hours:

By appointmen
t, Porter Hall
-
Room
504 (
phone: 740
-
593
-
1135 or email
:

showa
lte@ohio.edu
)


Academic Conduct
/Misconduct
:

The penalty for course
-
related academic dishonesty (i.e., cheating on exams, plagiarism, etc.) will be
failure of the entire course along with a report of the incident being sent to

University
Judiciaries.

Note
that
University Judiciaries
may impose additional sanctions, and
students may appeal academic
sanctions through the grade appeal process
.
Cell phones must be turned off during
class; violators will
be asked to bring treats for the class.


Attendance Policy
:

Attendance is highly recommended
.

Any absences must be well justified and explained to the instructor
in advance in order to make up any of the graded material.


Intellectual Property
:

The lectures, classroom activities, and all materials associated with

this class and developed by the
instructor are copyrighted in the name of
Allan Showalter on August 26, 2013.


A
ccommodations
for Students with a Disability
Policy:

Any student who suspects s/he may need an accommodation based on the impact of a disabilit
y should
contact the class instructor privately to discuss the student’s specific needs and provide written
documentation from the Office of Student Accessibility Services. If the student is not yet registered as a
student with a disability, s/he should co
ntact the Office of
Student Accessibility Services.




3

PBIO 450
0
/550
0
: BIOTECHNOLOGY AND GENETIC ENGINEERING

Fall 2013

-

Syllabus


Instructor: Dr. Allan Showalter


WEEK

DATE

CHAPTERS*

TOPICS


1

Aug. 27

1

Introduction


Aug. 29

2

DNA, RNA and protein synth
esis


2

Sept.
3

3

Recombinant DNA technology


Sept.
5

3

Recombinant DNA technology


3

Sept.
1
0

3

Recombinant DNA technology


Sept. 1
2

4

DNA synthesis, amplification and sequencing


4

Sept.
1
7

5

Bioinformatics, genomics and proteomics


Sept. 19

5

Bioinforma
tics, genomics and proteomics


5

Sept. 2
4

6, 7

Prokaryotic & eukaryotic gene expression


Sept. 2
6

-

Exam I


6

Oct.
1

9

Molecular diagnostics


Oct.
3

10

Protein therapeutics


7

Oct.
8

11

Nucleic acids as therapeutic agents


Oct.
1
0

12

Vaccines


8

Oct.
1
5

13

Genetic engineering of microbes


Oct.
1
7

13

Genetic engineering of microbes


9

Oct.
22

14

Bioremediation and biomass utilization


Oct.
24

16

Microbial insecticides


10

Oct.
29

-

Exam II


Oct
.
3
1

18

Genetic engineering of plants: methodology


1
1

Nov. 5

19

Engineering plants to overcome stress


Nov. 7

20

Engineering plant quality and proteins


1
2

Nov. 12

21

Transgenic animals


Nov. 14

21

Transgenic animals


1
3

Nov. 19

22

Regulating the use of biotechnology


Nov. 21

23

Societal issues in biotechnology


1
4

Nov
. 26

-

Class presentations


Nov. 28

-

No Class (Thanksgiving Break)


1
5

Dec. 3

-

Class presentations


Dec.
5

-

Class presentations



Dec.
12

(
Thursday
)

-

Final Exam (
10
:
1
0 AM)



4

*

Assigned readings are from chapters in your text,

Molecular Biotechnology (4
t
h

Edition)

©2010 by
Bernard R. Glick, Jack J. Pasternak and Cheryl L. Patten
.


5

SOME SUGGESTED TOPICS FOR YOUR CLASS PRESENTATIONS

(This list is merely a guide for potential
topics;

please consider other biotechnology/genetic engineering
topics that interes
t you at present or as the course progresses
.
Note that controversial
biotechnology/genetic engineering topics are
perhaps
the most interesting as you can present the
scientific information, the arguments “for” and “against” the technology, and your opinio
ns.)




Finding effective drugs to treat tuberculosis using a genetically engineered luciferase gene



Using the polymerase chain reaction to detect disease
-
causing agents (e.g., HIV)



Using restriction fragment length polymorphisms to detect genetic diseases



U
sing genetically modified organisms to clean up the environment



Production of human pharmaceuticals in the milk of genetically engineered farm animals



Treating human brain tumors by gene therapy with the thymidine kinase gene



The production of biodegradabl
e plastics in plants



The production of antibodies (i.e., plantibodies) in plants for medical use



The production of a particular human pharmaceutical in bacteria



Cause, detection, and treatment of a particular genetic disease (e.g., cystic fibrosis)



Genetic

engineering of transgenic fish with growth hormone genes



Production of recombinant tissue plasminogen activator for treating heart attacks



Engineering organisms with the jellyfish green fluorescent protein to follow development



The human genome project an
d the ethical considerations associated with it



Mammalian cloning (i.e., the cloning of sheep or human
s or pets
)



The use of gene knockouts to determine gene function



Enhancer trap experiments to locate tissue
-
specific promoters



Genetic engineering of herbi
cide
-
resistant plants



Genetic engineering of insect
-
resistant plants



Genetic engineering of “golden rice”



Genetically engineering
of
disease
-
resistant farm animals



Human gene therapy for SCID (Severe Combined Immunodeficiency Dis
ease)



Genomics
application(
s)



DNA microarrays

and the identification of genes associated with specific pathways



Genetic screening for human diseases



Proteomics

application(s)



Embryonic stem cell research and its applications for treating certain human diseases



Biology and molecular
biology of
Bacillus anthracis
, causative agent of Anthrax



Biology and treatment of H1N1 (swine) influenza



Marathon mice


--------------------------------------------------------------------------------------------------------
------------


INSTRUCTOR EVALUA
TION FORM FOR STUDENT PRESENTATIONS


STUDENT:


GRADE:



6

PRESENTATION FEEDBACK (i.e., comments on the organization and clarity of the talk, the quality
of the visual aids, the oral delivery/general "flow" of the talk, the scientific accuracy of the informati
on
presented, and whether sufficient background information was presented):

ASSIGNED HOMEWORK QUESTIONS

Email
your
answers to the homework questions for each assigned chapter to
showalte@ohio.edu

as a
Microsoft Word

attachment file.
Any diagrams or figures can be drawn by hand or with another
program and inserted into your Microsoft Word document.
Chapter answer sets are given equal grade
weighting and are
due each Monday by noon

for the past week’s
lecture material
.


Chapter

Questions

1

2,5,6
,
10

2

1,2,3,5
,12

3

1,2,3,
4,5,8,9,10

4

2,
3,
4,5,
8,9,10,11,12,13,16

5

1,2,3,7,10,11,14,15,17,19

6

1,
3,6,
8,20

7

1,
2,
3,
7,10,11,13,14

9

1,
3,
4,
5,9,12
,15,17,18,21

10

1,5,10,11,12

11

1,2,3,7,8,11

12

1,
2,3,4,5,7,15,16

13

1,4,9,10,12,17

14

2,5,12,13

16

3,4,9,15

18

1,2,3,4,5,6,10,11,15,16

19

1,2,
4,6,7,8,11,15,16

20

1,3,9,14,17,19

21

1,2,3,4,5,6,8,10,13,15

22

1,
2,4,5,6,8,10,13

23


3,4,6,7,9,12,13