AP BiologyCampbell Reading Guide Chapter 20: DNA Technology and Genomics

clattergumneckBiotechnology

Oct 23, 2013 (3 years and 9 months ago)

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Adapted from the work of Ms. Foglia




AP Biology







Name __________________

Campbell Reading Guide


Date ___________Period ___


Chapter 20: DNA Technology and Genomics


1.

Define biotechnology.



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2.

What is meant by “recombinant DNA technology?”


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3.

List some of the organisms we have been modifying for many hundreds of years.


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4.

Why are bacteria ideal workh
orses for biotechnology?


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5.

What are other organisms used in biotechnology?


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6.

How does gene cloning differ from human cloning?


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Adapted from the work of Ms. Foglia




7.

Why is DNA cloning considered an
important technology?


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8.

What are plasmids?


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9.

What is the function of restriction enzymes in bacteria?


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10.

How do bacteria protect their DNA from the effects of the restriction enzymes?


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11.

How do biologis
ts make use of restriction enzymes?


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12.


What is a genomic library?


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13.

How is cDNA different from typical eukaryote DNA?


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Adapted from the work of Ms. Foglia




14.

Describe the steps involved in cloning a gene.


























15.

How can transformed bacteria that carry genes of interest be identified and
isolated from the majority of non
-
transformed bacteria?


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16.

What can be accomplished with Nucleic Acid Hybridization?


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17.

What is the purpose of the Polymer
ase Chain Reaction?


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18.

List some advantages & uses of the PCR technique.




Adapted from the work of Ms. Foglia




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19.

How are DNA fragments of different sizes separated?


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20.

What is a RFLP? How are they made?


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21.

What does the technique of Southern Blotting accomplish?


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22.

What are some other techniques that build on the Southern Blotting technique?


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23.

What was the goal of the Human Genome Project?


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24.


List some of the most important things we learned by completing the H
uman
Genome Project.



Adapted from the work of Ms. Foglia





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25.

What is the Sanger Sequencing Method used for?


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26.

How does the shot
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gun approach differ from the whole
-
genome sequencing?


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27.

In the future, DNA chips may be used for regular diagnostics. What do the
florescent spots indicate when the chip is read?


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28.

How can DNA technology be used to diagnose a carrier of a genetic disorder?


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29.

What is the goal of gene therapy?


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30.

How

has forensics made use of DNA technology? Give a specific example.




Adapted from the work of Ms. Foglia




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31.

What is currently used by the FBI to do a DNA fingerprint in a criminal
investigation?


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32.

What technique has been used to modify agricultural plants?


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33.

List a few of the traits that have been engineered i
nto agricultural plants? Could
any of these pose an environmental threat?


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