Room Logs and Cleaning

clappingknaveSoftware and s/w Development

Dec 14, 2013 (3 years and 7 months ago)

52 views

JAMES MADISON UNIVERSITY


SOP Title:

Room L
ogs and Cleaning

SOP No.

JMU


12
-

100

Revision

0

Effective Date:

January 8, 2013

Page

1

of
7



1.

PURPOSE

1.1.

To establish basic guidelines for cleaning
rodent
rooms, corridors, labs and other areas inside the
vivarium.


2.

RESPONSIBILITY

2.1.

This SOP is the responsibility of the dep
ar
t
ment

for

the overall content
.

I
mplementation of this SOP
is
by all personnel working in the vivarium.

2.2.

Principal investigators (PIs) are responsible for following this document and ensuring all personnel
working on their protocols also adhere to its provisions
.

2.3.

Investigators, technicians, maintenance workers, repairmen, and any other personnel who have
occasional contact with research animals or animal facilities will comply with certain provisions of this
SOP
.


3.

DEFINITIONS

3.1.

PPE = personal protective equipment.
Includes but is not limited to masks, caps or bonnets, gloves, eye
protection, ear protection, lab coats, coveralls and shoe covers.


4.

MATERIALS AND EQUIPMENT

4.1.

PPE

4.2.

Brooms (no wood or straw)

4.3.

Mops (no wood)

4.4.

Dustpans

4.5.

Mop buckets

4.6.

Squeegee

4.7.

Cleaning solution
s

4.7.1.

Sani
-
Plex

4.7.2.

Clidox

4.7.3.

10% Bleach

4.7.4.

Roccal

4.7.5.

Quatricide

4.7.6.

Any
product
that

can be used to disinfect vivaria

(bacterialcide, fungalcide, sporocide,
viricide)

4.8.

3 ring binder

4.9.

log sheets

4.10.

Thermohygrometers

4.11.

Spray bottles


5.

PROCEDURES

5.1.

General

5.1.1.

Areas are swept and trash removed

daily.

5.1.2.

Floors are mopped clean and disinfected once a week or more often if necessary.

JAMES MADISON UNIVERSITY


SOP Title:

Room L
ogs and Cleaning

SOP No.

JMU


12
-

100

Revision

0

Effective Date:

January 8, 2013

Page

2

of
7


5.1.2.1.

Mop heads should be washed and disinfected with soap and bleach at least every two
weeks.

5.1.3.

Sinks are cleaned and disinfected once a week.

5.1.4.

Paper towel and soap dispensers

are refilled once a week or as necessary.


5.2.

Animal Room Care Procedures

5.2.1.

The
log sheet
is located
in

each animal room and is replaced every Monday, an
d filed in a
designated binder after review and stored for at least 3 years.

5.2.2.

All animal rooms will have
will have a log sheet that is filled out even if the room is empty.


5.2.3.

Animals held in

Room

G001E

are
designated to hold animals

either in a quarantine environment
or on a special project. The animals in these rooms are

not permitted to enter the animal faci
lity
until after they have cleared quarantine.
Animals held in these rooms will be checked after all
animal care has been completed in other animal holding areas.

Personnel may not enter the
main
vivarium

after entering
rooms
G0001E

until showering

and changing into clean clothes

5.2.4.

After completion the
log sheet
is collected every Monday
and checked for errors, incomplete
sections or problems.
The reviewer will try and r
esolve any issues before

sign
ing

and d
ating,

and then file in the binder.

5.3.

Room Sa
nitation

5.3.1.

Floors in all occupied animal holding rooms will be mopped
wit
h proper cleaning solution
weekly or more often as required
.

5.3.2.

Equipment in room

will be sanitized during room sanitation.

5.3.2.1.

Mops will be allowed to dry and MUST be stored on the holding
rack.

5.3.2.2.

Mops will not be kept in buckets.

5.3.2.3.

Mop heads will be replaced when dark grey or black colored, dirty, smelly, or ragged.

5.3.2.4.

Mops, buckets, carts and trash cans may be sanitized in the holding room or via the rack
washer.

5.3.3.

The hallways
w
ill be mopped or wa
shed daily with

proper cleaning solution.

5.3.3.1.

Sanitized at a minimum once monthly including floors and walls.

5.3.4.

The animal holding rooms must be thoroughly sanitized between shipments of animals and/or
every 2 weeks at a minimum.

5.3.4.1.

The entire room, including
walls
, and other permanent fixtures, will
be cleaned with
proper cleaning solution.

The walls can be cleaned using a sponge mop or similar mop
head.

5.4.

Animal Room Environment

5.4.1.

Rodent

rooms are required to maintain (dry
-
bulb) temperatures in a range of 64 to 79

F, with a
variability of


3 degrees from the set point (71

F). Temperature is monitored using a high/low
thermometer.

5.4.2.

All animal rooms
should have a minimum of
10
-
15 air changes per hour. Air changes are
measured every six months.

5.4.3.

Light cycle for all
rodent

rooms is set to a 12:12 cycle (e.g. 12 hours light, 12 hours dark). Time
on and off is

usually

7:00 AM and 7:00 PM, respectively. An automatic timer controls the light
cycle.

JAMES MADISON UNIVERSITY


SOP Title:

Room L
ogs and Cleaning

SOP No.

JMU


12
-

100

Revision

0

Effective Date:

January 8, 2013

Page

3

of
7


5.4.3.1.

Changes in light cycle due to breeding or study work (such as a reverse l
ight cycle)
should be incorporated into the IACUC approved protocol.

5.4.3.2.

Daylight savings time or electrical failure
usually
requires manual re
-
setting of the clocks

and timers
.

Start/Stop time should be changed for daylight savings time.

5.4.4.

H
umidity in each rode
nt room shall fall between 30


70% and be measured by a hygrometer.

5.4.5.

Any changes in Guide standards due to protocol approval will be posted on room door.

5.5.

Daily Procedures
in holding rooms

5.5.1.

Animals

are checked daily for deaths or signs of illness,
injury, an
d abnormal behavior.

5.5.1.1.

If not checked daily must be based on a IACUC approved protocol and posted on the
door of the room.

5.5.2.


Water availability is checked daily. Water bottles are replaced at least once a week or as
needed.
Lixits,
if used
,

are also checked daily for blockage, leaks or problems.

5.5.2.1.

One Lixit at the beginning and end of each row on a rack

should

be checked to make sure
there is constant flow.

5.5.2.2.

If a cage has flooded and the bedding is wet it will be changed out immediately witho
ut
waiting for regular cage change day.

5.5.2.3.

If a lixit is clogged or leaking the cage will be moved to another open spot and the lixit
will be repaired before using that space again.

5.5.3.

Food availability is checked daily. Food is refreshed (added to)
as needed to

prevent cages from
having no food available to animals.

5.5.4.

Food and water restrictions per protocol shall be posted on the outside of the holding room door.

5.5.5.

Temperature hi/lo readings are noted for each animal room.

5.5.6.

Oddities in the room are noted (smell, no
ise, other).

5.5.7.

The number of cage changes
, if done,

is noted.

5.5.8.

Laminar flow hood
s, cage changing stations

or fume hoods are cleaned using a 10
% bleach
solution or reasonable alternative.

5.5.9.

Trashcans are emptied and

plastic bag

liners are replaced.

5.5.10.

The floor
is swept and mopped
.

5.5.11.

Disinfectant spray

bottles

outside every room are checked and refilled.

5.5.12.

Clea
n lab coats are hung on coat racks.

5.5.12.1.

Dirty lab coats are taken to the laundry hamper in laundry room.

5.5.13.

Dirty cages, racks, and bins are taken to dirty cage wash
for cleaning
.

5.6.

Weekly Procedures
in holding rooms

5.6.1.

The floor is mopped disinfected at least once a week or more often if necessary.

5.6.2.

Laminar flow hood
pre
-
filter is checked and cleaned.

5.6.3.

Feed bin filled and feed type is noted on the form. This can be done le
ss often
than

once a week
depending on how often the bin is empty. Feed bin should be sanitized before putting new feed
into bin.

5.6.4.

PPE
stocked.

(gloves, masks, shoe covers, etc)

5.6.5.

Walls and fixtures
cleaned.

5.6.6.

Animal carts and other racks are cleaned and disin
fected.

5.6.7.

Census: Total number animals per room are counted each week.


JAMES MADISON UNIVERSITY


SOP Title:

Room L
ogs and Cleaning

SOP No.

JMU


12
-

100

Revision

0

Effective Date:

January 8, 2013

Page

4

of
7



6.

REFERENCES

6.1.

The Guide for Care and Use of Laboratory Animals, eighth e
dition, 2011, National Academy

Press


7.

REVISION HISTORY


Version No.

Brief Description of Changes


Created on Date

00

Creation of SOP

9
-
28
-
12





8.

FORMS AND APPENDICES


Form or Appendix No.

Title

JMU
-
12
-
100.F1

Animal Holding Room Log Sheet

JMU
-
12
-
100.F2

Hallways, Labs, Non
-
animal rooms Log Sheet



JAMES MADISON UNIVERSITY

SOP Title:

Animal Holding Room Log Sheet

SOP No.

JMU


12
-

100.F1

Revision

0

Effective Date:

January 8, 2013

Page

5

of
7


FORM 1



Month: _______________________


Dates: ___________________________



daily procedures

MON

TUE

WED

THUR

FRI

SAT

SUN

#
animals found dead








# cages labeled sick








Food adequate








Water adequate








Room Temp. in


F

(range 64
-
79


F)








Humidity 30


70%








Light timer
-

Time
verified








Oddities noted

(smell, noise, other)








# of cages changed








# of water bottles
changed








Laminar Flow Hood
Cleaned








Trash removed








spray bottles checked &
refilled








Lab coats swapped out








Floor swept








Technician Initials










Comments:

_________








__












_

Reviewed by: _________________________ Date: ___________




JAMES MADISON UNIVERSITY

SOP Title:

Animal Holding Room Log Sheet

SOP No.

JMU


12
-

100.F1

Revision

0

Effective Date:

January 8, 2013

Page

6

of
7








Month: ____
__________________________

Year
: _______________________


Weekly procedures

Week 1

Week 2

Week 3

Week 4

Week 5

# animals






Laminar hood

pre
-
filters cleaned






Floor mopped and
disinfected






Food bin filled

Food Type






Food bin sanitized






PPE stocked






Walls, door, equipment

cleaned






Racks dusted &
disinfected






Technician Initials







Comments:

_________



























__





_


Reviewed

by: _
________________________
Date
: ___________
JAMES MADISON UNIVERSITY

SOP Title:

Hallways, Labs, Non
-
animal rooms Log Sheet

SOP No.

JMU


12
-

100.F2

Revision

0

Effective Date:

January 8,
2013

Page

7

of
7


FORM 2


Month: ___________________________ Dates: ___________________________

Weekly procedures

MON

TUE

WED

THUR

FRI

SAT

SUN

Clean elevator








Sanitize surfaces








Floors mopped and
disinfected








Empty trash








Clean cabinets doors








Function check on equip








Walls, door and ceiling
cleaned








Empty and clean carcass
freezers


call for pickup








Wash lab coats








Technician
Initials











Comments:

____________

__________________________________________________________________________________________
__________________________________________________________________________________________
____________________________________________________________________________
______________
_____________________





Reviewed by: _________________________ Date: ___________