BIOL 251 1003 - Great Basin College

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Feb 20, 2013 (4 years and 3 months ago)

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General Microbiology, Biology 251






Fall
, 20
1
2


Syllabus Great Basi
n
College


Professor:
Dr. Rita Bagwe


Office:

101B






Email:

rita.bagwe
@gbcnv.edu






Phone:

775
-
753
-
2
005







Office Hours:

Mon,
thru
Th
u
r,
2:30


4:00









Or
by appointment.


Lecture Reading Assignments and Exam Dates


Chapter


Topic






1


T
he Microbial world

2
Chemical principles



3



Microscopy



4



Functional Anatomy of Cells



5



Microbial Metabolism



Exam I,
Tuesday
,
Sep

1
8




6



Microbial Grow
th



7



Control of Microbial Growth


10



Classification


11



Prokaryotes


12



Eukaryotic Microbes



Exam II,
Tuesday
,
Oct

1
6



13



Viruses


14



Disease and Epidemiology


15



Pathogenicity


16



Nonspecific Host Defenses


17



Immune Response



Exam III,
Tuesday
,
Nov

1
3



19



Immune System Disorders


20



Antimicrobial Drugs


21
-

26



Selected Pathogens & Diseases


27



Environmental Microbiology



Final Exam,
Tuesday
,
Dec
1
1


Reading assignments and exam dates are tentative, and may be changed

at the discretion of the
instructor. Any changes will be announced in class.


Text:
Microbiology, an Introduction
.
1
1
th

ed. Tortora, Funke, & Case.



ISBN 13: 978
-
0
-
321
-
55007
-
1

Lab Manual:
MicrobiologyLaboratory Theory & Application, Brief
.


20
10,
2
nd

ed.


Leboffe &


Pierce
.

ISBN 13: 978
-
0
-
89582
-
947
-
4

Grading System and
Miscellaneous
Policies:


The grade for this course will be based on two components: performance in lecture; and
laboratory. They will be weighted 70% for the lecture, and 30% fo
r the lab.


The
lecture grade
will be based on four, one hour exams, worth 100 points each. These exams
are not comprehensive.


The
lab grade

will be based on two practical exams worth 100 points each
;

Four
reports worth
20 points each
; A

Lab Notebook m
ust be maintained for
1
0 points;

Finally,
1
0 points of the lab
total will be based on my technical evaluation of your proficiency in the laboratory. Staining,
aseptic technique, use and care of equipment, are some of the factors that will
be judged.
Excessive tardiness/absences and careless behavior will also be factored into the technical
evaluation.



Attendance in lab is mandatory, there will be no make up labs.



The grading scale is as follows:




90
-

100% = A




80
-

89% = B




70

-

79% = C




60
-

69% = D




> 60% = F


All exams and lab practicals will be held on the dates listed in the syllabus. Any changes to that
schedule will be announced in class well in advance.
Missing an exam due to personal reasons
or illness must

be approved in advance if possible, or by contacting me within 24 hours of the
exam in the case of emergency.

Make up exams will be allowed and scheduled at my discretion.


Lab practicals are difficult to set up. As a result, make up lab practicals will

only be scheduled
in unusual circumstances. In these cases the format of the exam may change.
Do not miss lab
practicals!



Attendance will not be taken, but be advised:
Regular attendance is the single most important
thing you can do to get and maint
ain a good grade.


WebCampus


The syllabus and lab excercises based on handouts are posted on WebCampus. Should you loose
your syllabus,
it is always available there. Lab handouts should be printed out before coming to
that lab! I
will not

be checking the WebCampus email utility. Should you need to email me,
pl
ease use my regular GBC address
:
rita.bagwe
@gwmail.gbcnv.edu


This syllabus does not in any way represent a contract. It is a reflection of the intent of the
instructor, but do rec
ognize that it is an organic construct that may change as the semester
progresses. Any changes will be announced in class.



Lab Schedule



Lab #


Date
__
Exercise


Topic








1


Sep
4




Introduction





1
-
3



Aseptic Method of Transfer





3
-
1



Microscope






Pages 145
-
147


Bacterial Shapes




2


Sep 1
1


3
-
4



Simple Stains





3
-
6



Gram Stain





3
-
7



Acid
-
Fast Stain








D
emo Slides: Flagella; Endospore;

Capsule



3


Sep
18


1
-
2



Prepari
ng Culture Media





1
-
4



Streak Plates





2
-
3



Agar Slants





2
-
4




Broth Cultures





2
-
2



Colony Morphology

(read only)



4


Sep 25

4
-
1



Mannitol Salt Agar





4
-
6



Mac Conkey Agar





2
-
8



Effects of Temperature

2
-
10



Osmotic
Effects





2
-
6



O
2

-

Fluid Thioglycollate





2
-
7



O
2

-

Anaerobe Jar



5


Oct 2


5
-
2



Acid and Gas Production

F
rom Fermentation





5
-
11



Degradation of Polysaccharides





5
-
14



Degradation of Protein





5
-
7



Differential
Utilization of Citrate



6


Oct 9


5
-
3



Methyl
-
Red
& Voges
-
Proskauer Test
s





Handout


Indole Production





7
-
2



Antibiotic Evaluation


Oct1
6


Lab Exam I




Lab Schedule



Lab #


Date


Exercise


Topic








7


Oct 2
3


Handout


Unknown

Identification





5
-
4



Catalase Activity





5
-
21



Coagulase





5
-
20



Hemolysis of Red Blood Cells





Handout


Litmus milk



8


Oct
30


7
-
1



Snyder Test





Handout


Effects of Handwashing



9


Nov
6


7
-
6



Detecting Coliforms in Water





7
-
3



Plaque Assay



10


Nov 13


7
-
6



Detecting Coliforms in Water





8
-
2



UV


11


Nov 2
0


Hand out


Phage Assay


12


Nov 2
7

Hand out


Eukaryotic Microbes



Dec
4

Lab Exam I
I



Always read the lab exercise ahead of time. Knowing the

procedure allows the excise to go
more smoothly and quickly.


Note:

Handouts for Labs 6, 7 8
& 11
will be posted at WebCampus. It is
your

responsibility to
print them before the lab meets!


Important:

Lab is scheduled on
Tuesdays,
but many exercises wi
ll require that
observations be taken on cultures within 48 hours.
This will require your coming
into lab on

Wednesday

&
/or

Thursdays
to make those observations
.



Lab Reports


There will be
4

lab reports to complete for this course. The reports must be typed, double
spaced. The reports should follow the following outline:


Introduction

Give the background to the topic. Why is it important? Briefly explain what you
are going to do in the

experiment (i.e., state the
Objective

of the experiment).

Procedures

Explain what organisms, materials and methods were used in the experiments. How
did you do it?

Results

Present the results. Point out key or unexpected results.

Conclusion

What was the significance of your results? Explain the importance of the
experiment, and whether or not your outcome was consistent with what was expected.


Tables, graphs, etc. may be helpful. Do not remove pages from your manual. While this is not

an English class, bear in mind that correct grammar and spelling are important, and deductions
will be made for excessive mistakes in these areas.


Note that
one

of the write ups are based on more than one exercise. This does not mean that you
should wri
te a report for each exercise, rather, the exercises chosen are thematically related. So
the report should blend the topics into a single report.


Assignment







Date Due_______

1


Stains and Staining




Tuesday
,

Sep 2
5



(Ex
3
-
4
,
3
-
6
, &
3
-
7
)



2


Anti
biotic Evaluation




Tuesday
,

Oct

30




(Ex
7
-
2
)



3


Hand Washing Experiment



Tuesday
,

Nov
20


4


Identification of Unknown



Tuesday
,

Dec
4


Lab Notebook


Keeping an accurate permanent record of lab activities is at the heart of science. Random notes
on scraps of paper are a guarantee of confusion and poor performance.
Use a single bound
notebook, a composition book or spiral bound is fine. Remember that
your lab notebook will be
exposed to many microbes and chemical reagents, so keeping it separate from your lecture notes
is a very good idea. Your notebook should contain enough detail so that another person could
understand and repeat your work.

Proceed
ures that you will use repeatedly (gram stains, MR
-
VP, etc)

are good to have in your notebook.

Make your notes as you go along, don’t try to
reconstruct details from memory. In some cases amounts, volumes, and calculations are
essential

things to record.

A notebook is a good place to sketch out graphs or tables before
writing up your lab reports.

Key observations such
as reactions of specific microbes to stains or
metabolic tests can be quite useful when working on your unknown microbe.

Do not

rewrite
your notes to make them ‘prettier.’ Science is a messy process!

Extra Credit


Extra credit points are available for anyone with the energy and courage to take advantage of
them. Up to 20 points can be added to your lecture score by writing a
brief
,

inf
ormal
research
paper on one of the following diseases, and then
presenting a summary to the class
. The oral
presentation does not affect the grading, but is a means of sharing interesting and curious
information. The paper should be approximately 4 pages

long, typed, double spaced with no
typos or grammatical errors.


Dread Diseases:

Hepatitis B or C

Influenza

SARS

Cholera

Typhoid Fever

Rabies

Tetanus

Genital Herpes

AIDS/HIV

Gonorrhea

Elephantiasis

Syphilis

Chlamydia

Anthrax

Influenza

Yellow Fever

West Ni
le Fever

Amoebic Meningoencephalitis

Dengue Fever

Necrotizing fasciitis

Ebola/Hemorrhagic Fever

Beaver Fever

Viral Meningitis

Tuberculosis

E. coli

Food Poisoning

Brucellosis

‘Mad Cow Disease’/Bovine Spongiform Encephalopathy

Hanta virus

Small Pox

Monkey
Pox

Polio

Measles

MRSA

Catalog Description:


BIOL 251 General Microbiology (4)

A laboratory and lecture course emphasizing taxonomy,
morphology, physiology, infectious diseases, and ecology of microorganisms in addition to skills
in aseptic procedures,
isolation, and identification. Open to all life science majors and allied
health majors. Prerequisite: BIOL 190 or equivalent.


Course Objectives:




To provide a strong basis of microbiological knowledge including: the characteristics and
classification o
f microbes; microbial metabolism; microbes and disease; molecular genetics;
and interactions between microbes and the environment.



In the laboratory component, students will acquire a working knowledge of sterile
techniques, media preparation, staining, mi
crobial metabolism, and the isolation and
identification of microbes. This knowledge base will be directly applied to the analysis of a
variety of hands


Method of Instruction:


Live lecture and laboratory. Lecture may be presented via IAV.


Expected Le
arner

Outcomes:


Students will be able to apply knowledge and skills gained in a variety of situations: use of
aseptic technique in clinical and laboratory environments; identification of unknown organisms;
evaluation of risks associated with a variety of pathogenic micro
bes; comprehension of
molecular genetics and biotechnology;


of the basic factual content of biology, including relevant principles of chemistry, physics, and
geology. Beyond this factual base, students will be expected to comprehend this information
suf
ficiently to analyze and evaluate simple biologic situations that have not been directly
explicated in lecture or laboratory.


Measurement of Expected Learner Outcomes
:


Students will be assessed with three distinct tools: multiple choice lecture exams; l
aboratory
practical exams; and a written report on a laboratory experience. The lecture exams are designed
to test the students ability to recall matters stated in lecture and the text, define key terms, and
use concepts in a meaningful way. Laboratory p
racticals will asses both visual and textural
recall, as well as provide the opportunity for analysis and evaluation of concepts introduced in
lab with lecture information. The lab report will asses how well students have comprehended
and synthesized a ke
y laboratory experience, as well as their ability to communicate these
concepts via the written word.


Accommodations for Students with Disabilities
:


Great Basin College is committed to providing equal educational opportunities to qualified
students with
disabilities in accordance with state and federal laws and regulations, including the
Americans with Disabilities Act of 1990 and Section 504 of the Rehabilitation Act of 1973. A
qualified student must furnish current verification of disability. The Dire
ctor of Services for
Students with Disabilities (Julie G. Byrnes) will assist qualified students with disabilities in
securing the appropriate and reasonable accommodations, auxiliary aids and services. For more
information or further assistance, please c
all 775
-
753
-
2271.



Academic Honesty Statement


Great Basin College considers academic honesty one of its highest values.


A student who
obtains academic credit for work that is not the product of his or her own effort is being
dishonest and undermining th
e academic integrity of the college.


Students are expected to be the
sole authors of their work.


Use of another’s ideas must be accompanied by specific citation and
reference.


In addition, a learner may not submit the same work for credit in more than o
ne
course.


The disciplinary consequences of plagiarism and other forms of academic dishonesty
include non
-
acceptance of work submitted, a failing grade in the course, and/or or other
disciplinary action as outlined in Great Basin College’s Student Conduct

Policy.