Thermus aquaticus - Ms. Jackson's Blog

cattlejoyousBiotechnology

Dec 9, 2012 (4 years and 11 months ago)

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Biotechnology

DNA CLONING

Cloning: production of identical copies of DNA
through asexual means






Gene Cloning: production of identical copies of a
gene

Somatic Cell Nuclear Transfer

Gene Therapy

Recombinant DNA Technology

rDNA
: contains DNA from two more different
sources




Requires a vector (plasmid or virus used to transfer
foreign genetic material into a cell) and a plasmid
(small ring of DNA located in the cytoplasm of some
bacteria)

Recombinant DNA Technology

1.
A Restriction Enzyme
is used to cut DNA

2.
DNA piece is inserted
into the vector DNA
(bacteria or virus)

3.
Add DNA ligase to join
the fragments
together

4.
Insert the whole thing
into the vector



Recombinant

DNA

Polymerase Chain Reaction

PCR is a technique used
to create millions of
copies of a single gene


Uses DNA Polymerase
extracted from
Thermus

aquaticus

Analyzing DNA Segments

DNA Fingerprinting: using DNA fragments to
identify particular individuals


1.
Use restriction enzymes to “cut” desired
sequence

2.
Amplify the segments using PCR

3.
Run the segments through Gel
Electrophoresis

Gel Electrophoresis


DNA Fingerprinting Uses

-
Construct evolutionary histories

-
Identify deceased individuals from skeletal
remains

-
Determine paternity

-
Solve crimes when DNA evidence is found

TRANSGENIC ORGANISMS

-
organisms that have had foreign DNA inserted
into them


Bacteria


Plants


Animals

TRANSGENIC BACTERIA

TRANSGENIC PLANTS


TRANSGENIC ANIMALS

Gene Pharming:

Use of transgenic
animals to produce
pharmaceuticals in
the milk of the
females