Answer key 11.1 1. Apply the 4 steps commonly used to genetic ...

cattlejoyousBiotechnology

Dec 9, 2012 (4 years and 11 months ago)

4,368 views

Answer key


11.1


1.

Apply the 4 steps commonly used to genetic engineering experiments to describe
the cloning of a human gene


Human and vector DNA are cut and then combined to produce recombinant DNA. The
recombinant DNA is inserted into host cells. The
host cells reproduce, cloning the
human gene. The host cells are screened for the gene.


2.

Relate the role of DNA “sticky ends” in the making of recombinant DNA



The bases of the sticky ends are complementary, which allows the DNA from the two
different or
ganisms to combine



3.

Summarize how cells are screened in genetic engineering experiments,


The plasmids contain the gene for tetracycline resistance. Bacteria with the
recombined plasmid are grown in the presence of tetracycline. Only bacteria with the
p
lasmid survive.


4.

Evaluate the role of probes in identifying a specific gene

Probes are pieces of single stranded DNA or RNA complementary to the gene of
interest. The DNA fragments on the gel are made single stranded. The probe will
bind to any complemen
tary fragments. The genes are identified because the
probes are made with radioactive or fluorescent
-
labeled tags.


5.

A student performing electrophoresis on a DNA sample believes that her smallest
DNA fragment is the band nearest the negative pole of the g
el. Do you agree?


NO, disagree. DNA is negatively charged and opposite charges attract. The smallest
fragment will move fastest through the gel and thus farthest away from the negative
end of the pole.


6.

Many genetic engineering experiments are performed
in bacteria using circular
DNA molecules called:

D.

Plasmids