Genetic Engineering - Amazon S3

butterbeanscubaBiotechnology

Dec 12, 2012 (4 years and 10 months ago)

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Genetic Engineering

Good or Bad?

Genetic engineering is
the alteration of
genetic

code by artificial means,
and is therefore different
from traditional selective
breeding.



So what….?



Genetic engineering has created a chicken with
four legs and no wings.



What is next?



Genetic engineering therefore has few limits
-

except our imagination, and our moral or
ethical
code
.



Should we have an ethical code in
science?


Who should be the
writer of this ethical
code?


How far is too far?


Would you be the one
to make the decision
about what is
appropriate and what
isn’t?


What if genetic
engineering could
save your life or the
life of someone you
love? Would that
change your mind
about pushing that
ethical code
envelope?

Pigs and Insulin…


Used to use pigs and
cows for their insulin


We were able to extract
and purify their insulin
and give to people with
diabetes


Now we have genetically
engineered bacteria for
the same purpose.


Why is this significant?


How is it done?


The first step in transformation is to select a piece of
DNA to be inserted

into a vector. The second step is to cut that piece of
DNA with a restriction

enzyme and then ligate the DNA insert into the vector
with DNA Ligase. The insert contains a selectable

marker which allows for identification of recombinant
molecules. An antibiotic

marker is often used so a host cell without a vector dies
when exposed to a certain

antibiotic, and the host with the vector will live because
it is resistant.


What you need to remember…


Recombinant DNA is when you take one
gene and insert it into the genome of
something else.


The purpose is to get the gene you want
into something that doesn’t have it!


Think the bacteria making human insulin,
they don’t normally have that gene but
when put into the bacteria you now have
recombinant DNA!

Plasmids


Remember that circular DNA is
characteristic of prokaryotic cells


this is
a plasmid


Many times bacteria is used because it is
easy to find genes and insert them into a
less complex genome


Bacteria also reproduce at a rapid rate and
quickly give results of the recombinant
DNA


did it work or not?

Credits


http://www.globalchange.com/geneticengi
n.htm


http://www.endocrineweb.com/conditions
/diabetes/diabetes
-
what
-
insulin


http://blogs.discovery.com/.a/6a00d8341b
f67c53ef0134851d27fa970c
-
800wi


http://rpi.edu/dept/chem
-
eng/Biotech
-
Environ/Projects00/rdna/rdna.html