PC-I FORM - Planning Commission

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Dec 11, 2012 (4 years and 11 months ago)

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Revised 2005

PC
-
I FORM

GOVERNMENT OF PAKISTAN

PLANNING COMMISSION



UPGRADATION & STRENGTHENING OF

NATIONAL INSTITUTE FOR BIOTECHONOLOGY
AND GENETIC ENGINEERING (NIBGE)

FAISALABAD

(REVISED)

































April, 2006

BIOSCIENCES DIVISI
ON

PAKISTAN ATOMIC ENERGY COMMISSION

ISLAMABAD


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2

GOVERNMENT OF PAKISTAN

PLANNING COMMISSION



(Social Sectors)


1.

Name of Project:

Upgradation and Strengthening of National
Institute of Biotechnology and Genetics
Engineering(NIBGE)


2.

Location of Proje
ct:

National Institute for Biotechnology & Genetic
Engineering (NIBGE), Faisalabad.

3.

Authorities responsible for:



i)


Sponsoring


ii)


Execution


iii)


Operation & Maintenance



iv)

Concerned Federal
Ministry






Government of Pakistan




Pakistan Atomic Energy Com
mission (PAEC)



National Institute for Biotechnology and Genetic
Engineering (NIBGE), Faisalabad, PAEC


Ministry of Science & Technology

4.

Time required for completion of
Project

(in months)



36 months

4.

Plan Provision



Biotechnology is one of the p
riority areas in the
current Development Programme of Government
of Pakistan.



5.

Project Objectives and its
Relationship with Sectoral
Objectives.

























In a short span of mere twelve years NIBGE

has
demonstrated an unparalleled professionalism,
both in its R&D activities as well as in its
endeavours to upgrade its manpower. It has
achieved recognition both nationally and
internationally and has been awarded a status of
a
Centre of Excellence

in
Biotechnology

by the
Ministry of Science & Technology. In order to
keep a lead in biotechnology and to prepare the
nation as a practising and receiving unit of this
modern technology, following are the objectives
for strengthening and upgradation of this p
roject:

i
-

Biotechnology for Genetic
Improvement of Crops.

ii
-

Development of
Biofertilizers for
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3









































Increasing Sustainable Crop
Production.

iii
-


Establishment of Molecular
Diagnostic tests and Production of
therapeutic agents for Human and
Animal Health.

iv
-

To initiate, a
nd strengthen R&D on
cutting
-
edge biotechnologies such as
Protein Engineering, Nanobiotech
-

nology, Biosensors, Drug design and
Biopharming.

v
-

Production and Commercialisation of
Industrial Enzymes and chemicals.

vi
-

Establishment of Bioprocess
Technology.

vii
-


Bio
technological process for the
treatment of industrial effluent from
various industries.

viii
-

To develop trained manpower in
biotechnology by strengthening M.
Phil & PhD. Programmes



NIBGE as a Centre of Excellence is already
engaged in R&D activities related
to the
application of genetic engineering and
biotechnology to develop Agriculture, Health,
Industry and Environmental Biotechnology. This
project deals with the development in these areas
to an international standards so that it can fulfil
the demands env
isaged in these sectors for the
future and could carry out and provide facilities
for practising the cutting edge technologies which
are going to be the hall mark of the new
millennium. Certainly these efforts would go a
long way in improving the Socio
-
eco
nomic
resources of Pakistan.


Agriculture

1. Molecular characterisation of plant viruses


particularly affecting cotton, tomato, potato


and chilies etc.


2. Development of DNA based system for genomic


analyses of different economic crop.


3.
Development of biotic and abiotic stress


tolerance in crop plants.



4. Tailoring chloroplast genome to develop bio
-


safe economic characters in plants

against

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devastating problems.



5. Establishment of
in
-
vitro

techniques in


vegetati
vely grown crops and its application at


commercial level to make the project self
-


sustainable.



6.
Isolation and identification of beneficial


bacteria from roots, root rhizosphere and root


nodules of plants growing in different areas
of


Pakistan.



7. Selection of isolates showing high nitrogen


fixing activity, producing higher amounts of


phytohormones and phosphorous


solubilization, by Acetylene Reduction Assay


(ARA) using GC, HPLC and other serological,



microbiological, molecular and ultra structural


techniques.




8. Evaluation of the promising strains showing


beneficial effects for development of different


biofertilizers.



9. Culture collection and preservation of the


promising str
ains for their availability to


various universities, research organizations


and to the local farmers.



Health

1.

Establishment and improvement of Molecular
diagnostic (DNA
-
PCR based) tests for
Infectious and genetic diseases.



2.

Production of Monoclo
nal antibodies
(specifically directed against local isolates) for
the diagnosis and treatment of Typhoid,
Tuberculosis and Hepatitis B & C.



3.

Characterization and bulk production of
therapeutic agents (Insulin, Interferons,
Gonadotropin Releasin
g Hormone (GnRH) and
Growth Hormone) using biotechnological
approaches.


Industry

1.

Strains improvement in order to increase the
yield of cellulases, xylanases, lipases and
amylases through mutagenesis and
recombinant DNA technologies.

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2.

Directed evaluation f
or the improvement of
enzyme properties.



3.

Scale up of enzyme production in 100
-
500 L
and pilot scale fermenter using economical
substrate and medium composition.




4.

Comparison of these enzymes with
commercial enzymes.



5.

Use of enzymes in feed, food, text
ile and
detergent industries.



6.

Upscaling the coal biodepyritization process
for commercial application.



7.

Optimisation of the bioprocess for removing
organic sulphur from coal, furnace oil and
diesel.



8.

Identification of genes responsible for C
-
S
bond cle
avage in organic sulfur compounds
and their manipulation through genetic
engineering techniques for strain
improvement.



9.

Down stream processing through biosorption
for the recovery of precious metals from
lixiviant and removal of toxic metals from
industr
ial effluents.




10. Production of valuable compounds from less


valuable raw materials and detoxification of


highly toxic compounds through


biotransformation.



11.Exploitation of extremophiles for obtaining


industrially important
enzymes and other


biomolecules.



12.


Development of a culture collection which will
act as bioresource laboratory.


Environment

1. To develop customized effluent treatment
processes based on biotechnological
approach.




2. To establish facilities
to monitor Geno
-
toxicity


(including Comet assay) of industrial effluents,

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industrial products, chemicals, agrochemicals,


environmental samples, water samples or any


suspected material.



4.

Development of microbial consortia and


proces
s for the treatment of industrial waste
from textile, leather tanning,

pharmaceutical petroleum, fertilizer and

pesticide.


5.

To develop analytical facility for meeting the
various regulatory requirements.


6.

Description, Justification and
technical para
meters.

Existing Facilities:

The National Institute for Biotechnology and
Genetic Engineering (NIBGE), FAISALABAD has
infrastructure required to carry out
microbiological, molecular biological and
ultrastructural (transmission electron
microscopy) work. It

has four double storied
laboratories; each of them has a covered area of
775m
2
. The basic facilities available have been
tabled in
Annexure I.



Presently, the work at the institute revolves
around four sectors namely agriculture, health,
industry and env
ironment and is executed by
following six main research divisions:

1.Plant Biotechnology,

2.Biofertilizers,

3.Health Biotechnology,

4.Industrial Biotechnology,

5.Bioprocess Technology,

6.Environment Biotechnology.



Agriculture

In Pakistan NIBGE
is the leading centre
conducting molecular virology, genetic
engineering and DNA fingerprinting of crops.
NIBGE initiated a Cotton Biotech. Programme
which has resulted in developing sensitive and
rapid diagnostic virus identification assays which
have bee
n widely used by all the cotton breeders
and are being employed for developing engineered
resistance to the cotton leaf curl disease. Similar
studies in development of virus resistant tomato
and potato are at advanced stage of development.
Recently, a prog
ramme for development of insect
resistant cotton varieties, sugarcane and potato
using the Bt genes from
Bacillus thuringiensis

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has been initiated which will help in the
considerable reduction in the use of insecticides.
Another project being undertaken is

the
development of DNA marker technology (RAPDs,
SSR, AFLP) for the agronomically important traits
like CLCuV resistance, defence umbrella (red leaf
colour), nectariless, hairyless, fiber quality, etc.
Its utility has been proven in settling the case of
B
asmati rice export to Europe, Geographical
indication and GMO testing service to all the
exporters in WTO era.



Production of disease free plants through tissue
culture of potato and sugarcane is well
established. Production and dissemination of
disease f
ree propagating material at large scale
will further increase the per acre production.

The research activities of Biofertilizers division
are mainly focussed on biological N
2
-
fixation,
plant growth promoting hormone production and
phosphorous solubilizatio
n by various soil
microorganisms. The R&D activity involves
microbiological, serological, molecular and ultra
structural techniques. The group has recently
been strengthened through competitive research
grants from Islamic Development Bank (IDB),
Internati
onal Centre for Genetic Engineering and
Biotechnology (ICGEB) and Technical Co
-
operation Projects from IAEA.



NIBGE has developed biofertilizer with the trade
name of


BioPower
” for various grain and fodder
legumes and cereal crops, like rice and wheat.
T
he use of BioPower reduces the requirement of
chemical nitrogen fertilizer, increases crop yield
and has a healthy impact on the environment.
Several (
Brady
)
rhizobium

and PGPR strains have
been used for the preparation of BioPower for
various legumes and c
ereal crops. In legumes 30
-
40% increase in crop yield and 70
-
90% saving of
nitrogen have been achieved. Similarly, 15
-
20%
increase in crop yield 30
-
70% saving of N
fertilizer have been achieved in wheat and rice.
The biofertilizer for other crops like suga
rcane,
maize, cotton etc. will be developed which will not
only reduce the input/use of chemical
nitrogenous and phosphorous fertilizer but will
also decrease the load on the limited non
-
renewable energy resources.



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Culture collection and preservation of
various
(Brady)rhizobium
, phosphate solublizing and
PGPR strains will be carried out which will be
available at Biofertilizer Division, to all
researchers as well as for inoculum production
industry.



Health

Research and molecular diagnostic projects were

initiated on the diseases, most prevalent in the
country. The aims of these research projects are
to (i) access the use of PCR and DNA Probes for
the quick and reliable diagnosis of diseases (ii)
study the prevalence of these diseases in local
population
by biochemical and molecular
methods and also to (iii) provide diagnostic
facilities to the patients.

Tuberculosis (TB) caused by
Mycobacterium
tuberculosis

causes 26% of avoidable youth and
adult deaths in the developing world. In Pakistan,
about 1.5 mill
ion people are already suffering
from TB and more than 210,000 new cases are
reported each year. Treatment of this highly
contagious disease requires a multi
-
drug regimen
for prolonged periods of time (6
-
12 months),
which leads to a high level of noncompli
ance with
therapy. This lack of compliance has led to an
increase in drug resistant strains of
M.
tuberculosis.



The typing of Mycobacterial strains using the
IS
6110

element was performed according to the
standardized procedures as agreed by an
internatio
nal group of microbiologists. Variation
in DNA finger
-
prints were observed in the
M.tuberculosis
isolates.



Hepatitis c virus (HCV) is a significant cause of
chronic liver disease in Pakistan and worldwide.
Prevalence of HCV genotypes in Pakistan has not
been thoroughly investigated. Reverse
Transcriptase (RT)
-
Polymerase Chain Reaction
(PCR) and hybridization with genotype
-
specific
oligonucleotides to investigate the prevalence of
HCV genotypes in chronic liver disease patients.
HCV
-
type1 was found in 126

( 37.5%) pat i e nt s,
f o l l owe d by HCV
-
t y pe 3 and HCV t y pe 2 i n 56
( 16.66%) and 06 ( 02%) pat i e nt s r e s pe c t i v e l y.
Mi xe d i nf e c t i o n o f HCV g e not y pe s was al s o
o bs e r v e d.

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Typhoid is a worldwide problem. It is one of the
most common infectious diseases in Pakistan.
C
ommonly performed Widal test was found to be
highly non specific as about 40% healthy persons
gave positive reaction. Typhidot, a relatively new
diagnostic procedure to detect typhoid was
compared with PCR methodology. These results
clearly show that PCR i
s superior to Typhidot as
well.



Diagnostic Services for General Public

PCR
-
based Detection

(i)
Mycobacterium tuberculosis

(ii) Hepatitis C
virus (iii) bcr
-
abl translocation (for chronic
myeloid leukemia)

Detection of Chromosomal Abnormalities by
Karyot
yping

Facilities for the diagnosis of genetic disorders
are being provided to the general public. Patients
referred to our diagnostic laboratory were found
to have karyotypes for Down syndrome, Variant
of Turner syndrome, Robertsonian translocation
for Dow
n syndrome, Sex chromosome mosacism,
Philadelphia chromosome and Fragile X
syndrome. In future we are planning to establish
a PCR based test for Muscular Dystrophies and
Fragile
-
X syndrome.

Pre
-
Natal Diagnosis

The ARMS
-
PCR proved to be a powerful, rapid
a
nd cost
-
effective technique for carrier screening
and prenatal diagnosis of

-
thalassemia. The
present research project may facilitate the
establishment of comprehensive carrier detection
and prenatal diagnosis program for

-
thalassemia
in Pakistan.



Indu
stry

For the procurement of industrial enzymes as a
single commodity Pakistan spends huge chunk of
foreign exchange. According to broad estimate
Pakistan imports amylases, proteases, cellulases,
xylanases, lipases worth 2700 million rupees.
NIBGE has taken

a lead in the development of
new processes for the production of these
enzymes, based on indigenous microbial strains,
which not only may render independence of
patents owned by foreign companies but will also
enhance national economic savings. Besides
pr
oduction of alpha
-
amylase on pilot scale,
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process for the production of ethanol from agro
wastes have also been developed. Production of
other chemicals of significant importance, viz.,
citric acid, and lactic acid is also being carried
out using cheap ind
igenous raw materials.



Pakistan has huge reserves of different types of
ores. At NIBGE, various processes for the
recovery of metals from ores have been developed
and this technology
is ready for commercial
application. In this regard pilot scale model
p
rojects have been developed for biotechnological
recovery of uranium copper lead and zinc etc. It
has been estimated that cost of recoverable
quantities of different metals is about 6,000
million US dollars. For copper alone recovery of
this metal from tot
al Sandak’s (Blochistan
province) over burden is about 80 Million US
dollars. Adoption of these technologies on
national level will yield a huge economic impact
besides creating job opportunities and fortifying
poverty eradication efforts at governmental l
evel.
Similarly upgradation of fossil fuels for energy
generation and general use is a sought after area
of R & D. Coal biodesulfurization is highly
attractive alternative for economic gains besides
being an environmental friendly technology. We
have devel
oped coal desulfurization process at
pilot scale which has recently been handed over
to cement industry for commercial exploitation. It
has been estimated that this process is much
cheaper and less labour intensive than chemical
and physical process being
used at present.
Desulfurization of coal by this process saves
about 500 rupees per ton and also enhances its
calorific value to a greater extent.



Efforts are already under way for the
development of culture collection of national
scale at NIBGE, which w
ill act as bioresource
centre to meet demands of various academic as
well as research organizations and related
industries.



Environment

In Pakistan, concept of industrial waste
treatment and disposal is virtually non
-
existent.
Waste disposa1 in a scienti
fic way, especially use
of biotechnology in this respect can play a major
role, to save environment we live in. Biological,
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physical, chemical and thermal "cures" for
industrial toxins and carcinogens are part of the
answer. Now however, thanks to the prog
ress in
molecular biology, biochemistry and genetic
engineering, bioremediation (to enhance natural
biodegradation process) is shaping up a preferred
waste treatment method for the future. Because
it promises to dispose off industrial wastes cost
effective
ly and without creating other toxins,
bioremediation offers hopes for both physical
environment and industrial economy.
Carcinogenic/mutagenic and/or toxic materials
in effluents from textile, leather and petroleum
industries must be removed at industry ga
te
before entering into the sewage system. Using
micro organisms to combat these pollutions is
being practically applied in developed countries.
Applying them to solve pollution problem in
Pakistan is proposed in this project.



Training Manpower:

This pro
ject deals with the strengthening of Ph.D.
Programme which already exists at National
Institute for Biotechnology and Genetic
Engineering (NIBGE) Faisalabad to the
international level. After being formally affiliated
with the Quiad
-
i
-
Azam University, Islam
abad,
NIBGE has been able to fulfil its another
mandate to train high level manpower at post
-
graduate, doctorate and post
-
doctorate level. So
far five batches of students have been awarded
M.Phil degrees in biotechnology and a number of
students have enrol
led for Ph.D. degrees. This is
our modest effort in complementing the efforts of
the universities for development of scientific
manpower in the country. NIBGE also continued
to meet its mandated objective of imparting
training and share the expertise with
the
scientific community. Several specialized training
courses have been held. Two international
symposia/ workshops on Nitrogen Fixation have
been held in which more than 100 researchers
from 30 countries participated. Similarly NIBGE
hosted a two weeks t
raining course on behalf of
ICGEB which was attended by 24 scientists from
18 countries. All these activities have enabled
NIBGE to establish itself as a Centre of
Excellence among the international scientific
community.


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Description of Project:

The ultima
te goal of this project relates to
strengthening and upgradation of R&D activities
in four major areas i.e. agriculture, health,
industry and environment and teaching and
training in these fields and is proposed as given
below:



1.

1. Establish material de
pository for novel genes,
promoters, vectors set.

2.

2.


Ch l o r o p l a s t t r a n s f o r ma t i o n p r o j e c t t o b e
s t r e n g t h e n a n d e x p a n d e d t o i n c l u d e
b i o p h a r mi n g.

3. S e a r c h f o r n e w mi c r o b e s f o r b i o f e r t i l i z e r s a n d
t o e s t a b l i s h p r o d u c t i o n u n i t s i n e a c h p r o v i n c e

i n c l u d i n g No r t h e r n a r e a s a n d A J K.

4.

Consolidate activities of tissue culture for
potato, banana, sugarcane, date palm etc.

5.

Establishment of state
-
of
-
art facilities for
import/export of grain/food etc.(DNA
Fingerprinting, GMO Testing, Basmat
i/non
-

Basmati testing, etc).

6.

Establishment and improvement of molecular
diagnostic (DNA
-
PCR based) tests for infectious
and genetic diseases.

7.

Production of monoclonal antibodies
(especially directed against local strains) for the
diagn
osis and treatment of Typhoid, tuberculosis,
and Hepatitis B & C.

8.

Characterisation and bulk production of
therapeutic agents (Insulin, interferons,
Gonadotrophin Releasing Hormones (GnRH) and
Growth Hormone) using biotechnological
approaches.

9.

Strai
n improvement using the mutagenesis
and recombinant DNA technology for enzymes
and chemical production.

10.


Production of enzymes of industrial
importance on pilot
-
plant scale.

11.

Exploiting the use of enzymes in feed, food,
textile and detergent and oth
er allied industries.

12.

Upscaling the coal biodepyritization process
for commercial application.

13. Optimisation of bioprocess for removing
organic sulphur from coal, furnace oil and diesel
to render these fuels environment friendly.

14. Identification
of genes responsible for C
-
S
bond cleavages in organic compounds and their
manipulation through genetic engineering for
developing an efficient Biodesulfurisation
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process.

15. Bioprocessing of low
-
grade minerals.

16. Production of valuable compounds f
rom less
valuable raw materials and detoxification of
highly toxic compounds through
biotransformation.

17. Exploitation of extremophiles for obtaining
industrially important enzymes and biomolecules
of significant importance as well as searching for
novel

genes (Gene Mining).

18. To establish a Culture Collection which will
act as bioresource laboratory.

19.

To develop newly emerging field of Protein
engineering for exploring and better
understanding the gene function.

20.

To establish the nanobiotechnolog
y and
biosensor fields which shall be helpful in drug
delivery and design.

21.


To setup facilities for measuring National
Environmental Quality Standards.

22.


To establish facilities to monitor toxicity
(including Comet Assay) of industrial effluents,
ag
rochemicals, pesticides, etc.

23.


Development of microbial consortia and
process for the treatment of industrial waste from
textile, leather , pharmaceutical, petroleum,
fertilizers and pesticides residues.

24. Training Manpower for the future by
e
nrolment in M.Phil and Ph.D programme in
affiliation with Quaid
-
i
-
Azam University,
Islamabad. To provide trained manpower for
different public and private institutes and
entrepreneurs engaged in biotechnological R&D,
needs for on campus accommodation is b
ecoming
a necessity.


Achievements made by the institute

and basic
justification for the up
-
gradation of the project
:

Followings are the most significant achievements
made by NIBGE:



Developed viral resistant variety of cotton
for Cotton Leave Curl V
irus disease.



Developed system for genetic engineering of
crops like cotton, rice, sugarcane, tomato,
potato etc.



Established method for studying genetic
variability for different crops.



Developed and established DNA
fingerprinting for cr
ops helping exports of our
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agro commodities to Europe.



Developed Biofertilizers for various crops
with the trade name BioPower.



Developed PCR based methods for the
diagnosis of infectious and non
-
infectious
diseases.




Developed thermotoler
ant strains for sugar
industry for ethanol production.




Established Biogas process from agro
waste.




Scale
-
up production of industrial enzymes
employed in textile, food and feed industry.




Established biotechnological process for the
recov
ery of metals from low
-
grade ores on
commercial scale.




Developed coal Biodesulfurization process
for industry.




Discovered and deposited more than 54
genes recovered from extreme biotopes to
world databases as a bioresource.



Designed cust
omized processes for the
treatment of industrial effluents through
biotechnological innovations.



Developed technologies for the
bioremediation of soils and beaches after oil
-
spills.



Developed Genotoxicity tests (Comet Assay).




Have prod
uced so far 50 M.Sc., 140 M.Phil
and 50 Ph.Ds have completed/in progress.




The institute is ISO 1900
-
2001 certified.



Human Resource Development:


The Institute has always been very keen to
develop trained manpower, which is capable of
practising bi
otechnology and are equipped with
professional skills. This is achieved by a regular
programme of M.Phils and Ph.D. as well as
through short and specially designed workshops.
So far Institute has a regular programme of
having a course on Modern Techniques
in
Biotechnology in which 20
-
25 participants are
enrolled from all over the Pakistan, from
universities and various R&D institutions.



Publications:

The R&D activity at NIBGE resulted in 220
international and 100 national original research
papers in journ
als of international repute The
institute has a cumulative Impact Factor of 317
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Besides these many proceedings, progress
reports, activity reports are published regularly
Manuals of national and international
workshops/courses held by NIBGE are also
publis
hed and are available.



Linkages with Other Institutes /Agencies:

NIBGE has formal and informal arrangements for
cooperation with various institutions and
agencies within the country and abroad and is
well knitted within the federal and provincial
Resear
ch and Development system and
universities. Through its Director it is
represented in the Senate of Agriculture
University of Faisalabad, Board of Governor of
SBS, and IBGI, Peshawar and others. NIBGE is
also an affiliated Center of International Center
fo
r Biotechnology and Genetic Engineering,
Triestie, Italy, and had collaboration with
Australian ACIARS, IFS, CFC/ICAC, Third World
Academy of Sciences, Islamic Development Bank,
IAEA, Austria. IRRI etc. At national level the
institute is collaborating with

PARC, PCCC,
NARC, PSF, and Pakistan Academy of Sciences.



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Economic Impact












Sector

R&D Activity

Expected Benefit

Remarks

AGRICULTURE





Development of IR
Cotton

Saving of Rs. 3.4 Billion
of import bill of
insecticide/year

Development of IR Cotton wou
ld require
almost 50% less spray.

DNA
-
Finger Printing of
rice and other food
commodities

Export of rice to Europe
about Rs 5100 million

Due to Finger Printing of our Basmati
Rice, the export would be increased.

Biofertilizerz

An average benefit of
ab
out Rs. 2000
-
5000/acre.

Using Biofertilizerz, farmers shall save up
to 50% of chemical fertilizers which will
increase the yield.



INDUSTRY



Industrial Enzymes:
Xylanases, Celluloses
etc.



Rs 1600 million



Pakistan imports all these enzymes.
Manufactu
ring these enzymes, this
amount can be saved.

Citric Acid

Rs 200 million





Coal
Biodesulphurization

Coal worth Rs 5x10
5

million could be
utilized.

Pakistan has huge reserves of coal, by
this technology these can be utilized.



Bioleaching of low
Gra
de Ores

Only in Saindak, Cu
worth US $ 80
-
85
million can be
recovered.

Biotechnology can be used for low grade
ores. NIBGE has been involved in
Saindak Project.



HEALTH



PCR Diagnostic



There is market of Rs
4500 million which can
be benefited



Biote
chnology programme in health is
directed towards these objectives

Vaccine production

Market size is Rs. 5000
million



ENVIRONMENT



Industrial Effluent
Treatment



Under WTO every export
industry has to have
effluent treatment
plant.



Bioremediatio
n of Oil
Spills etc.

About Rs 10 million

Recently oil spill at Karachi has caused a
damage of worth Rs 600 million.

Revised PC
-
I NIBGE


17

Details of civil works equipments

and machinery and physical facilities

required for the project
:


Capital Cost:

(Million Rs)

L
ocal

F.E.C

Total

Remarks

Equipment and material

21.191

13
2
.
75
9

(135.55*)

153.95

(135.55*)

Annexure
-
III

Chemicals

5
.00

9
.00

14.00


Glassware and Plastic ware

-

7.173

7.173


Bachelors Hostel, Ladies Hostel,
Entrance, furnishing, air
-
conditioning and othe
r services

68.809


(26.597*)

-

68.809

(26.597*)

Annexure
-
IV & V

TOTAL

95.00

(26.597*)

1
48.932

(156.723*)

2
43.932

(183.32*)



7.

Capital Cost estimates (Rs.):

Total Capital Cost:


2
43.932

(183.32*)

million

Local Component:

95.00

(26.597*)

million

F.E. Component:

148.932

(156.723*)

million

(*) Approved Amount


Year
-
Wise/Component
-
Wise financial phasing: (Revised)

Sr. No.

YEAR

Local

F.E.C.

Total

1.

2005
-
06

15
.0
0

55
.00

70.00*

2.

2006
-
07

75
.00

75
.00

150.00

3.

2007
-
08

5.
00

18.9
32

23.932

Total

95.00

148.932

2
43.932



* Rs.66.00 million released as 1
st

installment (2005
-
06)


Year
-
Wise/Component
-
Wise financial phasing: (Approved)

Sr. No.

YEAR

Local

F.E.C.

Total

1.

2005
-
06

10.00

10.00

20.00

2.

2006
-
07

14.34

80.00

100.00

3.

200
7
-
08

2.257

66.723

63.32

Total

26.597

156.723

183.32


8.

Annual operating cost:



Total: 10.0 million

Repair & Maintenance: 4.0 million

Chemical: 5.0 million

Glassware: 1.0 million


Additiona
l Annual Recurring Expenditure at
the completion of the project will be borne by
PAEC.

9.

Demand Supply Analysis:

N/A

Revised PC
-
I NIBGE


18

10

Financial Plan:


Sources of financing

a)

Equity:

Indicate the amount of equity to be
financed from each source



Sponsors own resources



F
ederal government



Provincial government



DFI’s/banks



General public



Foreign equity (indicate partner
agency)



NGO’s/beneficiaries



Others


b)

Debt:

Indicate the local & foreign debt, interest
rate, grace period and repayment period
for each loan separately. The
loan
repayment schedule be also annexed.


c)

Grants:


d)

Weighted cost of capital:









Development Fund Government of Pakistan




11

a)

Project benefits and analysis


Financial:


Social:


Environmental:










b)

Project analysis



Quantifiable ou
tput of the
project



Unit cost analysis



Employment generation
(direct and indirect)





Impact of delays on project cost and
viability


Proposed programme of upgrading and
strengthening of R&D activities of NIBGE
will bring valuable benefits to the field of
a
griculture, health, industry and
environment. Increase in the per unit yield
of crops, production of industrial enzymes
and chemicals from indigenous raw
material and utilization of indigenous
resources like local for energy production
and improvement of h
ealth will directly
improve the socio
-
economic resources of
the country.





About five senior scientists will be
employed permanently whereas M.Phil,
Ph.D. and research scholars will be
engaged as contigent workers.

Revised PC
-
I NIBGE


19

12

Implementation of the project



Start
ing and Completion date
of the project



Item
-
wise/year
-
wise
implementation schedule in line
chart co
-
related with the
phasing of physical activities.



Starting Date: 01
-
07
-
2005


Completion Date: 30
-
06
-
2008


13

Management structure a
nd
manpower requirements



Administrative arrangements
for implementation of the
project.



Manpower requirements
during execution and
operation of the project be
provided by skills/profession.



Job description, qualification,
experience, age and salary of each

job be provided.


14

Additional projects/decisions
required



Indicate additional
projects/decisions required to
optimize the investment being
undertaken on the project.


Revised PC
-
I NIBGE


20

Certified that the project proposal has been prepared on the basis of
instructions
provided by the Planning Commission for the preparation of
PC
-
I for Social Sector projects.


Signature and Designation of the officers sponsoring the
Project





Prepared by:









Dr. Yusuf Zafar, TI

Director

National Institute for Biotechnology and

Gen
etic Engineering, Faisalabad.







Dr. Syed Javaid Khurshid

Director Project Coordination

(Biosciences Division)

Pakistan Atomic Energy Commission

Islamabad


Checked by:





Dr. Syed Javaid Khurshid

Director Project Coordination

(Biosciences Division)

Pakistan Atomic Energy Commission

Islamabad




Recommended by:





Dr. Kauser Abdulla Malik, HI, SI, TI

Member (Biosciences & Admin)

Pakistan Atomic Energy Commission

Islamabad




Approved by:









Mr. Anwar Ali, HI

Chairman

Pakistan Atomic Energy

Commission

Islamabad.


Revised PC
-
I NIBGE


21


Annexure
-
I


Details of Equipment Available



Sr. No

Name of the Equipment

1.





Incubators

2.





Autocalves

3.





Autoclaves 150L

4.





Fermentors

5.





Fermentors

6.





Fermentors (Braun)

7.





Fermentor (Ne
w Brunswick)

8.





Centrifuges (Beckman)

9.





Ultracentrifuges (Beckman)

10.




UltracentrifugesBeckman

11.




Ultracentrifuges (Hitachi)

12.




Microfuges

13.




Chromatographic Columns

14.




Cold Cabinets

15.




Freezers
-
20 C

16.




Freezers
-
70 C

17.




Water Baths
-

T
emp Controlled

18.




Laminar Flow Hoods

19.




Fume Hoods

20.




Shaking Incubators

21.



Shaking Incubators Temp Controlled

22.




Magnetic Stirrers

23.




Fraction Collectors

24.




Cell Sonicators

25.




Spectrophotometers

26.




Electrophoresis System Horizontal

27.




Electrophor
esis System Vertical

28.




Pulse Field Gel Electrophoresis

29.




Laser Scanning Densitometer

30.



UV/VIS Photo documentation System

31.




Probe Counter

32.




Liquid Scintillation System LKB

33.



Thermal Cycler Perkin Elemer

34.



CO2 Incubator

35.



Liquid Nitrogen Storag
e System (Taylor Wharton)

36.



DNA Sequencer ABI

37.



Biolistic Gun Biorad

38.



DNA Synthesiser Pharmacia

Revised PC
-
I NIBGE


22

39.



FPLC

40.



Microscopes

41.



Double Beam Spectrophotometer

42.

Atomic Absorption Spectrophotometer Varion

43.

pH meters

44.


Graphite Furnace for Atomic Abs
orption Spectrophotmeter

45.


Elementgal Analyzer

46.


FTIR








































































Revised PC
-
I NIBGE


23



Annexure
-
II


Trained Manpower Available at NIBGE



S.No.

Name



Academic Degree(s) with major subject



Director

Dr. Yus
uf Zafar, T.I.


Ph.D. (Plant Physiology)






Biofertilizers Division

1. Dr.Fauzia Yusuf Hafeez

Ph.D. (Microb.Physiology)

2.

Dr.Sohail Hameed


Ph.D. (Biological Sciences), M.S.(Biology
-

Canada)

3. Dr. M.Sajjad Mirza


Ph.D. (Molecul.
Microbial. Ecol.)

4. Dr.Ghulan Rasul


Ph.D. (Chemistry)

5. Fatiha Mubeen


M.Phil (Biochemistry), Ph.D. fellow UAF.

6. Sumaira Yasmeen


M.Sc. (Botany), Ph.D. fellow QAU (2003
-
7).

7. Arsalan H.Zaidi


M.Sc. (Biology), Ph.D. fellow Netherland.

8. Asma Aslam



M. Phi
l (Botany), Ph.D. fellow QAU. (2003
-
7)

9. Naima Aslam



M.Sc. (Botany), Ph.D. fellow QAU (2003
-
7)





Plant Biotechnology Division

1.

Dr. Aftab Bashir


Ph.D. (Agronomy, USA)

2. Dr.Shahid Mansood


Ph.D. (Plant Virology U.K)

3. Zahid Mukhtar



M.Sc.
(Botany) Ph.D. Fellow P.U, Lahore.

4. Dr. Shaheen Asad


Ph.D. (Biochemistry)

5. Dr. Naseer A.Saeed,


Ph.D. (Plant Molecular Biology/Plant Biotechnology)

6. Muhammad Saeed


M.Sc. (Plant Breeding & Genetics), (Ph.D. Fellow Australia 2002)

7. Dr. Muha
mmad Arif


Ph.D. (Molecular Marker/Genome Mapping)

8. Saif
-
ur
-
Rashid



M.Sc. (Plant Breeding & Genetics), Ph.D Fellow Australia 2002
-
6)

9. Anjuman Arif



M.Sc. (Biochemistry); Ph.D. Fellow P.U., Lahore.

10. Mazhar Hussain


M.Sc. (Plant Breeding & Genet
ics)

11. Sher Afzal Khan



M.Phil (Biotech)

12. Shazia Iram



M. Phil (Mol. Biology)

13.

Dr. Mehboob
-
ur
-
Rahman

Ph.D. (Plant Breeding & Genetics)

14. Imran Amin



M. Phil (Biochemistry)

15. Muhammad Asif



M. Sc. (Hon.)(Plant Breeding & Genetics), Ph.D. fe
llow B.Z. Univ.

16. Ch. M. Moddassar Ahmad

M. Phil (Microbiology)


Health Biotechnology Division

1.

Dr. Javed Anver Qureshi

Ph.D. (Biochemistry)

2.

Dr. Rubina Tabussum

Ph.D. (Chemistry)

3.

Dr.Shahid Mahmood Baig

Ph.D. (Molecular Biology)

4.

Dr.Abdul Haq


Ph.D. (Medical Microbiology)

5.

Zarqa Saif


M.Sc. (Zoology), Ph.D.fellow Univ.of Adeliode, Australia (2003
-
6)

6.

Dr. Nasir Ahmad


Ph.D. (Biochemistry)

7.

Dr. Fazl
-
e
-
Rabbi


Ph.D. (Biochemistry, U.K.)

8.

Dr. Abida Raza


Ph.D. (Biochemistry)

9.

Dr. Ayesha
Sharif


M.Phil (Biotechnology), M.B.B.S.

10.

Zafar Iqbal


M.Phil (Mol.Biology)

11.

Ayesha Azhar


M.Sc. (Hematology)



Revised PC
-
I NIBGE


24

Indusrial Biotechnology Division

1.

Dr.M.Ibrahim Rajoka

Ph.D. (Chemistry)

2.

Dr. Shoukat Parvez


Ph.D. (Biological Sciences) Czech Repu
blic

3.

Dr. Farooq latif


Ph.D. (Chemistry)

4.

Dr. Romana Tabussum

Ph.D. (Biological Sciences)

5.

Dr. Hamid Rashid


Ph.D. (Zoology)

6.

Raheela Parveen


B.Sc. (Chemical Engineering)

7.

Hassain Ali Amin


M.Sc. (Chemical Engineering)

8.

Muhammad Farhan


M.Phi
l (Molecular Biology)



Bioprocess Technology Division

1.

Dr M. Afzal Ghauri


Ph.D. (Biotechnology/Microbiology)

2.

Kalsoom Akhtar


M.Sc. (Chemistry), PhD Thesis submitted.

3.

Muneer A. Anwar


M.Sc. (Chemistry), Ph.D. fellow Netherland.

4.

Shazia Khaliq


M.Phil. (Biotechnology)



Environmental Biotechnology Division

1.

Dr.Zafar M.Khalid


Ph.D. (Biological Sciences)

2. Dr.Qaiser M.Khan


Ph.D. (Biotechnology, Molecular Biology)

3.

Dr. Samina Iqbal


Ph.D. (Biotechnology, U.K.)

4.

Mohammad Afzal


M.Phil
(Biotechnology)

5.

Anwar ul Haq



M.Sc. (Chemistry)

6.

Razia Tahseen



M.Phil (Biotechnology)

7.

Ameena Mobin


M.Sc. (Microbiology)

8.

Amer Jamal Hashmat


M.Sc. (Environmental Engineering)


Biotech Interdisciplinary Division

1.

Dr. M.Sarwar Khan


Ph.D. (Plant

Molecular Biology, UK)

2.

Dr. Irshad Hussain


Ph.D (Chemistry, UK)

3.

Dr. Mazhar Iqbal


PhD (Chemistry, UK)

4.

Moazur Rehman


M.Phil (Biotechnology), Ph.D. (Leeds
-
continued)


Statistical Cell

Jafar Hussain


M.Sc. (Statistics)


Advisor to Member Bioscienc
es:

Dr. Ahmad M. Khalid


Ph.D. (Biotechnology)






Revised PC
-
I NIBGE


25

Annexure
-
III


LIST OF EQUIPMENT REQUESTED



Sr.No

Equipment

Quantity
(No.)

Amount

Approved

(Rs. Million)

Amount
Revised
(Rs. Million)

1.

Series 20 0 autosampler for HPLC

1

0.60

0.60

2.

Fluorescent dete
ctor for HPLC

1

0.60

0.60

3.

Vacuum Gel Dryer System

1

0.20

0.20

4.

Pipetman Sets

(set of six)

6

0.40

0.40

5.

DNA Sequencer

2

13.00

13.00

6.

Real time PCR

2

7.00

7.00

7.

Biofocus 300 automated Capillary
electrophoresis unit

1

5.00

5.00

8.

GC
-
MS wit
h ECD/FID Detectors

1

7.00

7.00

9
.

2D

乍k

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獡浰汥爬⁖慲楡扬攠se浰牡瑵牥潤畬敳m
⡖(⤬⁰畬獥⁦楥汤⁧牡F楥湴
偆䜩潤畬攠
慮搠獯晴睡牥a

N

ㄸ⸰N

㌰⸰〪

㄰N

䅵瑯獡浰汥爠景爠噡物潮⁁瑯浩挠䅢獯牰瑩潮A
印散瑲潰桯瑯浥瑥爱〯㈰

1

1
.50

1.50

11.

Libraries for FTIR

4

1.00

1.00

12.

Atomic Force Microscope (AFM)

1

12.00

12.00

13.

Microarray System

1

28.00

28.00

14
.

Confocal laser scanning microscope

With cryofrequency unit, laser sources,
ceramic lenses, UPS and image software.

1

7.0
0

13.00*

15.

LC
-
MSMass spectrophotometer

1

10.00

10.00

16.

Temperature Controlled Cell disintegrator

1

0.50

0.50

17.

MALDI
-
TOF DE PRO

1

13.50

13.50

18.

PD
-
QUEST 2
-
D Analysis software

1

0.75

0.75

19.

GS
-
800 Calibrated Densitometer

1

1.00

1.00

20.

Protein IEF System

1

1.00

1.00

21.

Chiller
-

Industrial Scale

1

4.00

4.00

22.

Upright Freezer


o

2

2.00

2.00

23.

Ice Making Machines

2

0.40

0.40

24.

Cathode lamps for Fe, Zn, Pb, Cu, Ag, As,
Se and Cr.

8

0.32

0.32

25.

Microlab 300

1

0.40

0.40

26.

Refrigerated CO
2

Incubator

1

0.78

0.78



TOTAL



135.55

153.95

*

Cost of only two equipments (Sr. No. 9 & 14) is revised to include some
accessories which were missed in the original PC
-
I. These are essential for
research work on nanotechn
ology and localization studies.

Revised PC
-
I NIBGE


26


Annexure
-
IV


Detail of Building

Constructed At NIBGE

(Approved)


Sr. No

Name of Building

Covered
Area

(Sq Feet
)

Estimated Cost

Approved

@ Rs. 700/sft

(Million)

1.

Bachelor Hostel

10000

7.000

2.

Female Hostel

7545

5.282

3.

Male Hostel

10000

7.000

4.

Staff Hostel

5464

3.845

5.

Reception & entrance gate

2500

1.7500

6.

Approach Road

-

0.450
*


Total Cost:

25.327


Contingency @ 5%

1.266


TOTAL COST:

26.597





*
Amount @ Rs.375 Running sft

Revised PC
-
I NIBGE


27

Annexure
-
IV



B


Detail o
f Building Constructed At NIBGE (
Revised
)


Sr.No

Description

Covered Area

(sft)

Approved
PC
-
I cost

@ Rs.700
/sft (Million)

Revised PC
-
I cost

(Rs. Million)

Remarks


A. CIVIL Works





1.

Bachelor Hostel

10000

7.000

11.363

Estimated Cost based on
Pak PWD

2004 with 25%
up tender trend

2.

Female Hostel

7545

5.282

9.963

--
do
--

3.

Male Hostel

10000

7.000

13.456

--
do
--

4.

Staff Hostel

5464

3.845

8.204

--
do
--

5.

Reception & Entrance gate
NIBGE

2500

1.750

2.2300

As per actual tender cost

6.

Approach Road
to NIBGE

0.450

2.407

--
do
--


Total A


26.597

47.623



B. Services



External electrification of Sr. 1 to 4


2.200

Estimated Cost based on
Pak PWD 2004 with 25%
up tender trend




Ext. electrification of Sr. 5


0.684

As per actual t
ender cost




Sui Gas @5% of Sr. 1 to 5


2.261

-




Furnishing 220/sft Sr. 1 to 5


7.812

-




Air conditioning 26 no. 2tons reversible split
AC @ 40,000/unit


1.040


-





External Pavement and boundary wall of Sr.
No 1


1.041

Estimated Cost based on
Pak PWD 2
004 with 25%
up tender trend




External Pavement and boundary wall of Sr.
No 2


0.836

--
do
--




External Pavement and boundary wall of Sr.
No 3 & 4


2.035

--
do
--


Total B


17.909



C. Total A + B


65.532



D. Contingency @ 5% Of

C


3.277



Sub Total A+B+D


68.809



Equipment

135.5500

153.950



Chemicals, Glassware and Plastic ware

21.1730


21.1730



Cost of Approved PC
-
I

183.320




Cost of Revised PC
-
I


243.932



Allocation for Year 1 (2005
-
06)

Release 1
st

installment

70

66






Revised PC
-
I NIBGE


28

Annexure
-
V


PLANS OF BUILDINGS



1.

Bachelor’s Hostel

2.

Female Hostel

3.

Males Hostel

4.

Staff Hostel

5.

Entrance



Revised PC
-
I NIBGE


29

Annexure
-
Final


REVISION OF PC
-
I FOR UPGRADATION OF NIBGE


PC
-
I form is now prepared on the revised Proforma and the price of equipment as well as
construction cost is also calculated on prevailing rates and revised estimates are as under
(Annexure
-
I, Annexure
-
II)


Sr.No

Description

Covered Area

(sft)

Approved
PC
-
I cost

@
R
s.700

/sft

(Million)

Revised PC
-
I
cost

(Rs. Million)

Remarks


A. CIVIL Works





1.

Bachelor Hostel

10000

7.000

11.363

Estimated Cost based on
Pak PWD 2004 with 25%
up tender trend

2.

Female Hostel

7545

5.282

9.963

--
do
--

3.

Male Hostel

1
0000

7.000

13.456

--
do
--

4.

Staff Hostel

5464

3.845

8.204

--
do
--

5.

Reception & Entrance gate
NIBGE

2500

1.750

2.2300

As per actual tender cost

6.

Approach Road to NIBGE

0.450

2.407

--
do
--


Total A


26.597

47.623



B. Service
s



External electrification of Sr. 1 to 4


2.200

Estimated Cost based on
Pak PWD 2004 with 25%
up tender trend




Ext. electrification of Sr. 5


0.684

As per actual tender cost




Sui Gas @5% of Sr. 1 to 5


2.261

-




Furnishing 220/sft Sr. 1 to 5


7.812

-




A
ir condi
tioning 26 no. 2tons reversible
split
AC @ 40,000/unit


1.040


-





External Pavement and boundary wall of Sr.
No 1


1.041

Estimated Cost based on
Pak PWD 2004 with 25%
up tender trend




External Pavement and boundary wall of Sr.
No 2


0.836

--
do
--




External Pavement and boundary wall of Sr.
No 3 & 4


2.035

--
do
--


Total B


17.909



C. Total A + B


6
5.532



D.
Contingency @

5% Of C


3.277



Sub Total A+B+D


6
8.809



Equipment

135.5500

153.950



Chemicals, Glassware a
nd Plastic ware

21.1730


21.1730



Cost of Approved PC
-
I

183.320




Cost of Revised PC
-
I


2
4
3.932



Allocation for Year 1 (2005
-
06)

Release 1
st

installment

70

66