Genetic Engineering

boundarydapperBiotechnology

Dec 16, 2012 (4 years and 6 months ago)

241 views

Genetic Engineering

How do you cut down DNA into
managable

pieces?


Cut the DNA into
fragments at certain
points using restriction
enzymes


View segment using gel
electrophoresis


Transformation of bacteria


Take a desired gene and use
restriction enzymes to splice
out the gene.


Add that gene to a bacteria
plasmid


Now human gene mixed with
foreign DNA (recombinant
DNA)


DNA will bind together
regardless of species


Insert plasmid back into
bacteria


Insulin & Human Growth
Hormone production

Polymerase Chain Reaction (PCR)


Sometimes scientists want to isolate a gene
and make many copies of that gene.


Heat a piece of DNA to separate the strands


As DNA cools, new nucleotides are added


DNA polymerase enzyme used is from bacteria

Transgenic organisms



Organisms with foreign DNA


Plant uses


GMO


Toxins to kill insects


Resistance to herbicides


Resistance to viral infections


Grow faster

& larger


More vitamin A or beta
-
carotene


Animals


Cows produce more milk


Pigs produce more lean meat or omega
-
3 acids


Faster growing animals


Organ production

Gene Therapy


Take a gene of interest and add it to a vector
to be delivered into the human body


VIRUS IS THE VECTOR


Need to take a virus that is harmless and will not
reproduce


Very controversial and dangerous.