Slides - ICELW

blessinghomoeopathAI and Robotics

Nov 30, 2013 (3 years and 8 months ago)

84 views

Collaborative
E
-
L
earning
application for
electro
p
oration
-
based
biomedical applications


June 12th
-
14th, 2013


Selma
Corovic

Background and motivation

-

Electroporation of cells and tissues


human, animal or plant
cells for different biomedical
and

biotechnological applications




-

Electroporation based biomedical applications require
multidisciplinary expertise: oncologists, physicists, engineers…





Collaborative E
-
Learning application

clinical electrochemotherapy

-

cancer treatment

Oncology

Biomedical engineering

E
-
Learning enviroment

(EBTT)

E
-
learning application on

cells and tissues

Research

Clinical trials

Improving the content

based on results

Col
l
ecting

the knowledge

Designing

the content

E
-
Learning application

+

Evaluation

(pedagogical efficiency and usability)

E
-
Learning enviroment

Electroporation based technologies and treatments

International SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE

November 18
-
24, 2012
, Ljubljana, Slovenia

Lectures

Practical work

Lectures were recorded

molecules

cell membrane

Electroporation

Important parameter for effective electroporation


local electric field distribution E [V/cm]!

Cell membrane

Cell

Tissue

Electroporated

Local electric field distribution

E

>
Erev

(a)
The electroporation of cell membrane first occurs within
the cell area facing the electrodes

(b)
Different electroporation regimes:

reversible
Erev
<
E
<
Eirrev

and irreversible

E

>
Eirrev

UTI

= −1
.
5
r

E
cos (
φ
)


Electroporation is used in
:


-

clinical electrochemotherapy
,
non
-
viral gene
electrotransfer

for gene therapy
and DNA vaccination, transdermal drug delivery


-

liquid food sterilization
,
water treatment
,
electrofusion

of cells




Applications of Electroporation

biomedical

time

Electrodes

Electric pulse
generator

Tumour

Chemotherapeutic
surrounds the cells

Increased membrane
permeability allows
access to the cytosol

Pores reseal

Injecting chemotherapeutic

Electric pulse application

Electrochemotherapy =

= chemotherapy + electroporation


Local electric field distribution:

drug

injection

+

U/d

Result

Protocols used in clinical electrochemotherapy today include:

8 pulses of 100
µ
s duration, f=1 Hz or f=5 KHz,
U/d =1300 V/cm

Protocols and local electric field distribution in tumors

Importance of an adequate local electric field for

successful tissue electroporation (example:
un
successful tumor treatment
)

Clinical use of electrochemotherapy

R
eimbursed
in:

Germany
,
UK
,

Italy
,

Portugal
,

Denmark
,

Greece
,

Slovenia.


Polan
d



in progress

for
reimbursement. ECT approved by Minister of
Health.

Number of
patients

No. of clinical
centres

2006

130

SOP


2007

300

37

2008

600

53

2009

800

55

2010

1
000

83

2011

15
00

9
2

2012

3000

1
08

Clinical use of electrochemotherapy

E
-
Learning enviroment

(EBTT)

E
-
learning application on

cells and tissues

Research

Clinical trials

Improving the content

based on results

Col
l
ecting

the knowledge

Designing

the content

E
-
Learning application

+

Evaluation

(pedagogical efficiency and usability)

Laboratory of
bio

cybernetics

Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd
Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic,
Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek,
David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl,
Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic.

(Missing from the photo:
Karel Flisar,
Marija Marcan,
Andraz Polak
)

http://lbk.fe.uni
-
lj.si