Biochemistry 301/Spring 1998 - Vanderbilt School of Medicine

blaredsnottyAI and Robotics

Nov 15, 2013 (3 years and 10 months ago)

68 views

V
ERSION
11/15/13




Biochemistry 301 module: Molecular Structure and Function

Spring 2011: Tue & Thu / 1:00
-
2:00 P.M.

Room: 836 RRB

R. Armstrong, Organizer

(Ext. 3
-
2920, r.armstrong@vanderbilt.edu)





Text (
requ
ired
): Frey and Hegeman (2007)
Enzymatic Reaction Mechanisms
, Oxford University Press, New York.



Molecular Structure and Function. This course considers the use of biochemical methods to answer
important questions of function in systems involving two int
eracting species. Topical examples of
protein
-
protein, protein
-
ligand, protein
-
nucleic acid and nucleic acid
-
nucleic acid interactions are
considered. Each example illustrates the use of multiple complementary approaches, which may
include mutagenesis, kin
etic, chemical, spectroscopic, and in vitro selection methods. SPRING. (2)
Armstrong, Egli, Guengerich, Sanders, Schey.

DATE

TOPIC

INSTRUCTOR

January



Thu 13

Chemical reactivity of protei
ns

Guengerich

Tue 18

Chemical reactivity of proteins

Guengerich

Thu 20

Stereochemistry: Pyridine nucleotides, prochirality, chiral methyls

Guengerich

Tue 25

Catalytic Nucleic Acids

Egli

Thu 27

Catalytic Nucleic Acids/RNA Interference

Egli

February



Tue 01

Optical Spectroscopy

Armstrong

Thu 03

Introduction to catalysis, transition state theory

Armstrong

Tue 08

Steady
-
state kinetics

Armstrong

Thu 10

Steady
-
State kinetics

Armstrong

Tue 15

Pre
-
steady
-
state kinetics

Armstrong

Thu 17

Pre
-
steady
-
state

kinetics

Armstrong

Tue 22

Pre
-
steady
-
state kinetics

Armstrong

Thu 24

Lids, tunnels and reactive intermediates


Armstrong

March



Tue 01

Genomic Enzymology

Armstrong

Thu 0
3

EXAM 1 (1
-
3 PM)

Armstrong


Spring Break


Tue 15

Dynamic equilibrium and sim
ulation methods

Guengerich

Thu 17

Kinetic isotope effects

Guengerich

Tue 22

Mechanism
-
based inactivation

Guengerich

Thu 24

Mechanisms of prosthetic groups: pyridoxal, thiamine

Guengerich

Tue 29

Mechanisms of prosthetic groups: flavoproteins, electron t
ransfer

Guengerich

Thu 31

Oxygenases

Guengerich

April



Tue 05

Mass Spectrometry of Proteins

Schey

Thu 07

Mass spectrometry of proteins, proteomics


Schey

Tue 12

Mass spectrometry of proteins, proteomics

Schey

Thu 14

H/D exchange mass spectrometry

Armstrong

Tue 19

Random mutagenesis/molecular breeding

Guengerich

Thu 21

Model enzymes (biomimetics, catalytic antibodies) & unusual conditions (extremophiles)

Guengerich

Tue 26

Protein Stability

Sanders


Thu 28

EXAM 2 (1
-
3 PM)

Armstrong