The Complete Urinalysis with Automation, 2 Lab

basicgratisMechanics

Nov 5, 2013 (3 years and 5 months ago)

77 views

MLAB 1311 UA & Body Fluids Laboratory Exercise #
7

CRg

1

LABORATORY
7
:

The Complete
Urinalysis with Automation
, 2
nd

Lab


revised for
Fall
201
2


PreLab Preparation:


R
eview the first ‘Complete Urinalysis with Automation’ lab’s contents

including
Objectives
.


Points

Points are awarded for Admission Tickets, Skills, including general lab requirement
s
.
Most Lab Report
Forms are due at the conclusion of the lab period; check with the lab instructor for excep
tions.


Agenda

1

Review related textbook
readings

and techniques from previous UA lab exercises.

2.

Perform
routine
instrument
maintenance, quality control and operation procedures
required in
preparation of performing routine
urinalysis as
outlined in
the first automation lab
.

3.

Perform complete urina
lysis on five (5) specimens including color, transparency, UA chemical

analysis and microscopic exams.

4.

Clean work area, restock supplies if needed.


Equipment and Supplies

1.

Siemens Clinitek Status+
urine c
hemistry
a
nalyzer

2. Urine specimens (5 o
r more)
, appropriate urine controls with expected results sheet.

3.
Multistix 10 SG reagent strips

4. Centrifuge, TS Meter
,
Microscope
, c
entrifuge tubes & racks, Sharpie marker, Kim
-
wipes,

microscope slides and cover glasses.

5.
3% SSA

solution, Clinitest, Acetest, and Ictotest suppli
es, reagent tablets and product
inserts
.

6. Color reference pictures of urinary sediment
.


Quality Control

1
.

Review specific
manufacturer’s recommendations and QC information presented in first
automation
lab.

2.

Positive and Negative Controls will be analyzed during each laboratory

3.

Control results must be placed on the student report sheet. Any discrepancies must be brought to


the instructor’s attention.


References

Same as
the first ‘Comple
te Urinalysis with Automation’ lab.


Procedures and
Sample Analysis

1.

Obtain and organize supplies and specimens. All control and patient urine samples must be well

mixed and at room temperature. Lab coat and gloves are required for all course labs.

2.

Repeat procedures of
the first ‘Complete Urinalysis with Automation’ lab.



MLAB 1311 UA & Body Fluids Laboratory Exercise #
7

CRg

2

Name

_________________________ Date ______________________

QC LAB REPORT FORM


/ 10 pts


Control 1 Lot
#

____________

Control 1 Exp Date__________


Control 2 Lot
#

___________

Control 2 Exp Date_________

Control 1

Control 1

expected
results

Control 2

Control 2
expected
results

Within Range?

Yes or No

(If No, must bring to instructor’s
attention and add a comment
-

as to
course of action.)

Whether yes
or no, you must include
your initials!

Specific
Gravity
Refractometer

DI Water


__________






Automated

Dipstick




Glucose






Bilirubin







Ketones







Sp. Gravity







Blood







pH







Protein









Urobilinogen






Nitrite







Leukocyte

Esterase






Microscopic (only if indicated by manufacturer
and directed by instructor)





ADDITIONAL TESTING

Perform the following tests as directed by the instructor.


*Reminders: 1. In the ‘Comments’ box, you must state
‘Yes’ or ‘No’ whether or not the controls have given expected results and include your
initials. 2. ‘If at any time, a control sample does not give the expected result, you must note it under ‘Comments’ and bring

it to the
instructor’s attention.

Back
-
up

/
Confirmatory
Tests


Control 1

Control 1

Expected results

Control 2

Control 2

E
xpected results

*Comments:

Within Range?

Yes or No?

3% SSA (
for protein)






Acetest

(ketones)






Ictotest

(bilirubin)






Clinitest


(reducing substances)






MLAB 1311 UA & Body Fluids Laboratory Exercise #
7

CRg

3

Name

_________________________

Date ______________________


2
nd

AUTOMATED URINALYSIS REPORT SHEET


/ 2
5 points

Students have the option of using their own urine

specimen as their unknown # 5.



Specimen #


1

2

3

4

5


Patient
Name

Patient

ID

#

_________

_________

________
________

________
________

________
________

__________
__________

Physical
Properties:

Color






Transparency






Dipstick:


Glucose






Bilirubin






Ketones






Sp Gr






Blood






pH






Protein






Urobilinogen






Nitrite






Leuko
cyte

Ester
ase






Microscopic

Casts (LP)







Mucous (LP)






Squamous Epi (HP)






Other Epi (HP)







WBC (HP)






RBC (HP)






Crystals (HP)








Bacteria (HP)






Yeast (HP)






Trichomonas (HP)






Other