Laboratory of Microbial Ecology and Technology LabMET - Research topics and expertise

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Dec 6, 2012 (4 years and 11 months ago)

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Laboratory of Microbial Ecology and
Technology (LabMET)

Ghent University

Belgium


Nico Boon

Research topics and expertise




Ghent:

Population: 250,000

Established: 630 A.D.

In 1350: Largest city of W Europe after Paris

Historical and cultural richness

Where is Ghent situated?

Ghent

University


Founded in 1817 by William I, King of
the Netherlands


1990’s
-

today: uninterrupted growth


11 faculties, education and research in
all scientific
branches


32.000 students and 7.100 staff
members


Open
, committed and pluralistic
university


Broad international perspective


Located in the cultural and economical
heart of Europe

Faculty


CES


Department



Faculty of Bioscience Engineering


Now: 1600 Bioengineer students


BaMa structure


Bachelor (3 yr.) + Masters (2 yr.)



Centre
for

Environmental

Sanitation


International program for masters


Each year 10 scholarships (300 applicants)


See website:
www.cms.ugent.be



Department Biochemical and Microbial Technology


Lab. Industrial Microbiology and Biocatalysis


Lab. Microbial Ecology & Technology




Laboratory of Microbial Ecology and Technology


Head of department
:
Willy
Verstraete


Teaching staff:

N
. Boon,
T. Van de
Wiele
, K.
Rabaey

(09/11)


Post
-
doc
: 9


Scientific staff
:
25
PhD
-
collaborators


Technical staff
: 8 persons


Administrative staff
: 3 persons


Master students
:
20

LabMET mission statement

Microbes as multicellular organisms

« Study mixed
microbial communities &
interactions
to better understand ecological
processes

and use and/or
steer

the microbial
community in
biotechnological

applications

»


Main
research areas


Applied Microbial Ecology (community/stability)


New ecological theories


New tools and analysis


Microbial
Resource
Management


Environmental biotechnology (water & soil)


(Micro)
-
pollutant clean up


Methane production/removal


Microbial based materials


Bio Electrochemical Systems (BES)


Gastro
-
intestinal
microbiology


Food/Feed/Probiotics/Prebiotics


Bacteria
-
host interactions


Involvement in ULIXES

Two work packages


WP4 (13 PM)


T
ask
4.3. Collection 3.


Biopalladium

nanoparticles precipitating
prokaryotes




WP7 (26 WP)


WP leader


Task 7.3. Development of novel
biopalladium

nanoparticles dechlorination processes






8

Biological production of
nanoparticles


Metal salt



Reducing
agent


Capping
agent


M
+

M
+

M
+

M
+

M
+

M
+

M(
0)

F
d
h
H
F
d
h
H
F
d
h
H
F
d
h
H
C
O
H
O
H
C
O
H
O
H
C
O
H
O
H
C
O
H
O
H
C
O
H
O
H
C
O
H
O
H
C
O
H
O
H
C
O
H
O
H
2

H
+
2

e
-
Pd
II

2

H
+
2

e
-
2

H
+
2

e
-
2

H
+
2

e
-
Pd
II

Pd
0

P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
P
d
0
E
n
z
y
m
a
t
i
c
r
e
d
u
c
t
i
o
n
o
f

P
d
I
I
C
O
2
C
O
2
C
O
2
C
O
2
Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

Pd
0

1 µm

10 nm

Pd
II

Pd
II

Pd
II

Pd
0

NaCOOH

CO
2

+ Pd(II)

Pd(0)


Bacterial cells
couple the reduction of soluble Pd(II) to
the oxidation of an
electrondonor


Deposition
of this biogenic Pd as
nanoparticles on
the cell
wall and
periplasmatic

space

Production of Pd
nanoparticles

=
BioPAD

Shewanella

oneidensis

(De
Windt

et al., 2005;
Environ
Biotechnol
, 90:377
-
389;LabMET)



Loading the bio
-
Pd with H
2
/formate=> strong reductive
capacities






Applicable for chlorinated solvents, PCB’s,
dioxines
, pesticides (
lindane
), nitrate, perchlorate,
EE2,
diatrizoate

+ H
2

H

H

H

H

H

H

H

Application of
BioPAD

as catalyst


Cl

Cl

Cl

H

H

H

H

H

H

H

+ Cl
-

H

H

H

H

Cl

Cl

Cl

Cl

Cl

Cl

H

H

H

H

H

H

H

H

Bio
-
palladium
is catalytic: PCBs

H

+ H
+

+ Cl
-

+ H
+

+ Cl
-

+ H
+

+ Cl
-

H

PCB contaminated sediment

No BioPAD

50 mg/L

BioPAD

500 mg/L

BioPAD

De Wind et al 2005 Environmental Microbiology, 7, 314

325
-
LabMET

Tasks and challenges within ULIXES

Isolation of “collection 3” in WP4 (Task 4.3):


New metal precipitating strains from marine environments


Metal Precipitating Marine Bacteria: MP
-
MB


In situ or ex situ production of catalysts


New hydrogen forming
bacteria from marine
environments


Hydrogen Producing Marine
Bacteria:
HP
-
MB


In situ activation of the MP
-
MB


Experimental procedures


Screening in batch for
Pd

precipitating bacteria from partners


Characterisation (SEM, TEM, EDX, …)


Screening in batch for
hydrogen producing bacteria
from
partners


Focus on activity and substrate specificity






16

Tasks and challenges within ULIXES

Development of PCB dechlorination product in WP7
(Task 7.3):


Marine environmental applications never explored


Catalyst poisoning


Activation of
Pd

in the marine systems


Proof of principle using H
2

gas


Using in situ biogenic H
2
(backup: MFC based H
2
production)


Recovery and release of
Pd

in the environment


Background values in the sediments of
Pd
?


Immobilisation of
Pd

(polymers)


Recovery of
Pd






17

Recent research in relation to
Ulixes

Production and characterization of biological palladium


Chidambaram, D.,
Hennebel
, T.,
Taghavi
, S., Mast, J., Boon, N.,
Verstraete
, W., van der
Lelie
, D., &
Fitts
, J.P. (2010). Concomitant
microbial generation of palladium nanoparticles
and hydrogen to immobilize chromate. Environmental Science & Technology, 44,
7635
-
7640.


De
Windt
, W., Boon, N., Van den
Bulcke
, J.,
Rubberecht
, L.,
Prata
, F., Mast, J.,
Hennebel
, T., &
Verstraete
, W. (2006).
Biological
control of the size and reactivity
of catalytic
Pd
(0) produced by
Shewanella

oneidensis
.
Antonie

Van Leeuwenhoek International
Journal of General and Molecular Microbiology, 90, 377
-
389
.

Catalytic action of
biological
palladium in reactors


Forrez
, I.,
Carballa
, M., Fink, G., Wick, A.,
Hennebel
, T.,
Vanhaecke
, L.,
Ternes
, T., Boon, N., &
Verstraete
, W. (2011). Biogenic
metals for the oxidative and reductive removal of
pharmaceuticals, biocides and iodinated contrast media
in a polishing
membrane bioreactor. Water Research, 45, 1763
-
1773.


Hennebel
, T., Benner, J.,
Clauwaert
, P.,
Vanhaecke
, L.,
Aelterman
, P.,
Callebaut
, R., Boon, N., &
Verstraete
, W. (2011).
Dehalogenation of environmental pollutants in
microbial electrolysis cells with biogenic palladium
nanoparticles. Biotechnology
Letters, 33, 89
-
95.


Hennebel
, T., De Corte, S.,
Vanhaecke
, L.,
Vanherck
, K.,
Forrez
, I., De
Gusseme
, B.,
Verhagen
, P.,
Verbeken
, K., Van der
Bruggen
, B.,
Vankelecom
, I., Boon, N., &
Verstraete
, W. (2010). Removal of diatrizoate with
catalytically active membranes
incorporating
microbially

produced palladium nanoparticles. Water Research, 44, 1498
-
1506.


Hennebel
, T., De
Gusseme
, B., Boon, N., &
Verstraete
, W. (2009). Biogenic metals in advanced water treatment. Trends in
Biotechnology, 27, 90
-
98.


Hennebel
, T.,
Simoen
, H., De
Windt
, W.,
Verloo
, M., Boon, N., &
Verstraete
, W. (2009).
Biocatalytic

dechlorination of
trichloroethylene with bio
-
palladium in a
pilot
-
scale membrane reactor
. Biotechnology and Bioengineering, 102, 995
-
1002.


Hennebel
, T.,
Simoen
, H.,
Verhagen
, P., De
Windt
, W., Dick, J., Weise, C.,
Pietschner
, F., Boon , N., &
Verstraete
, W. (In press).
Biocatalytic

dechlorination of
hexachlorocyclohexane

by immobilized bio
-
Pd

in
a pilot scale dynamic bed reactor
. Environmental
Chemistry Letters.


Hennebel
, T.,
Verhagen
, P.,
Simoen
, H., De
Gusseme
, B.,
Vlaeminck
, S.E., Boon, N., &
Verstraete
, W. (2009). Remediation of
trichloroethylene by bio
-
precipitated and
encapsulated palladium nanoparticles
in a fixed bed reactor. Chemosphere, 76, 1221
-
1225




UGent Researchers on
Ulixes


Nico Boon


Group leader


One person to hire (2011
-
2014)


Post
-
doc on
Ulixes


Daily research and management


Tom
Hennebel


Post doc; synthesis and applications of Bio
-
Pad


Bart De
Gusseme

(2011)


Phd

student; reductive removal of
micropollutants


Simon De Corte (2011
-
2013)


Phd

student;
new types of biogenic
nanocatalysts

(bimetallic particles)


Synthia

Maes

and
Steffie

Pardaens

(2011
-
2012)


Master students


Extra possibility within ULIXES

Continuous High Pressure Reactor

Bioresource

Technology
,
2010,
101
, 3132
-
3138.

Ir. David van der Ha (PhD)

Ir. Bert
Bundervoet

(MSc)

Gwynet

Leyre

(MSc)

Prof. Dr.
ir.

Willy
Verstraete



More information:



Acknowledgements

www.LabMET.Ugent.be







Cees

Buisman
: WETSUS, Leeuwarden and
Wageningen

University, The Netherlands

Lars
Angenent
: Cornell University, USA

Ruth Ley: Cornell University, USA

Nathalie
Delzenne
:
Université

Catholique

de Louvain, Belgium

Jeroen

Raes
: Free University of Brussels, Belgium

Tom Curtis: Newcastle University, UK

Marc
Strous
: Max Planck Institute for Marine Microbiology, Germany

Mark Van
Loosdrecht
: Delft University of Technology, The Netherlands

Ken
Timmis
:
Braunschweig

University of Technology, Germany

Willy
Verstraete
: Ghent University, Belgium