rDNA ASSIGNMENT SHEET 2011-2012

alarmduckBiotechnology

Dec 12, 2012 (4 years and 4 months ago)

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BIOLOGY A
:
RECOMBINANT DNA TECH
NOLOGY


Assignment Sheet:
Mrs. Kreiselman

Last Update:
12.04.11

Thursday


THANKSGIVING



Friday




Monday

Nov.28

DNA Extraction

DNA Extraction LAB

* DNA Extraction questions
(per group)

*
Homework: Recombinant
DNA 1 (rDNA1):

Read pages
264
-
267

1. What are restriction enzymes?
Where do they naturally exist?

2. Figure page 265: Why did
enzymes 1 and 2 result in
different DNA lengths?

3.
How does Electrophoresis
separate DNA fragments from
each other?

4.

How can one tell if a
restriction enzyme really cut a
DNA molecule

as expected
?
(see figure 9.3).

5. What are

“Sticky ends”?


6. Go to p.277 Fig 9.11. Answer
“Apply”?



Due: Wednesday

Tuesday

29

Staff

Application:
Restriction,
Transformation

*
Paper Plasmid:
Marking


Wednesday

30

Application: Restriction,
Transformation

*
Paper Plasmid: Cutting
and pasting

*
Analysis of paper plasmid
activity (per group)



Thursday

Dec 1

Application:
Electrophoresis and DNA
fingerprinting

* Paper Plasmid


cont.

* Recombinant “Plasmid”

*Homework rDNA2:

DNA
Fingerprinting; pages 272
-
274

1. What are three uses for DNA
fingerprinting (p.274 bottom)?

2. How does DNA
fingerprinting work? (2 steps,
p.267, 272)

3. Why do they choose to cut in
‘non
-
coding’ regions of the
DNA (p.272)?

4. Why is it easier to prove
innocence rather than that guilt?
(p.274).

Due:
Monday

(postponed)

Friday

2

Application: DNA
Fingerprinting

*
Gel electrophoresis
demonstration

* DNA Fingerprinting:
reading from “DragonFly”
Book


Monday

5

Application: DNA
Fingerprinting


Application:
GMOs

* Video: “Crime Seen”

* Video:

Harvest of Fear


-

Bt Corn

-

Breeding vs. transgenics

-

Environmental
effects


* Discussion

* Videonotes:

Crime seen




*
Homework rDNA3:

Genetic
engineering p.275
-
279

1. What is “recombinant DNA”?

2. What is a transgenic
organism?

3. What are plasmids? How are
they useful for genetic
engineering?

4. How does genetic engine
ering
depend on a shared genetic code
(=p.277 “Summarize”)

5. What are at least three uses
for genetic engineering?


Due:
Wednesday


* Videonotes: Harvest of Fear

Tuesday

6

Staff
Development

Application: GMOs

* Video
s
:

Harvest of
Fear


-

Breeding vs.
Genetic
engineering.

-

Bt Corn



* VideoNotes (in notebook)


Wednesday

7

Application:
Bioinformatics

* Video:

-

“Genetic modification”

* Reading: Genomics.

Genomics, DNA
sequencing.

Homework:

FINAL Review Guide


Due: Final Exam

Thursday

8




Friday

9

Review: Vocabulary



Monday

12

Review: Biomolecules,
Cells, Molecular Biology



Tuesday
-
Thursday

13
-
15

FINAL EXAM: Periods 1,6



Friday

16






*
Homework rDNA4:


Biozine p.292
-
294

1. “A cure worse than the disease”: explain why.

2. “Customized drugs”:

According to what? Explain.

3. “Gene Therapy”: What are three potentials technologies?

4. What are three limits for use of pharmacogenomics?

EXTRA CREDIT:

Go to
http://www.biologybiozine.com

Select a biotechn
ology
-
related item (article, career, ethics..) and write a paragraph about what you
have learned.

Due: Thursday


* Video: “Finding cure is hard”

* Discussion: Gene Therapy