Lesson #2 Problem set Genetic Engineering Genetics Agro 315

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Dec 12, 2012 (4 years and 6 months ago)

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Lesson #13

Problem set Genetic Engineering Genetics Agro 315

Questions due
Mon
day,
April 16, 2012
,
11:59pm


1.

Below are steps performed to make
an

E. coli bacteria that can make
human insulin protein for the health care market.


a.

Use heat
shock or electroporation to get the recombinant human insulin
gene to go into a living E. coli cell.

b.

Grow the E. coli in fermentation culture, extract and sell Humulin.

c.

Modify

the human gene and give it a promoter that will be turned on in E.
coli cells.

d.

E
xtract the DNA from a human who does not have diabetes

e.

Isolate and clone (make copies of) the gene encoding the insulin protein


List these steps in their proper order:_____________________________


View the “
Who wants to be a genetic engineer
” and “
Gene Gun

animations before doing #2 and #3
.


2.

Below are steps used performed to ma
ke Corn that makes a protein toxic
to European Corn Borers.


a.

Culture Bt bacteria from the soil environment where they are found in
insect rich environments.

b.

Cross the transgenic corn plants with other corn plants to pass on the Bt
transgene.

c.

Coat copies
of the modified Bt gene onto gold particles.

d.

Grow corn plants from the “shot” corn cells

e.

Extract the DNA from cultured Bt bacteria

f.

Isolate and clone the insect toxin encoding gene from the Bt bacteria.


List these steps in their proper order:______________
_______________


_____3. Assume you are a genetic engineer and want to make a transgenic rice
plant to have
an

enzyme that will make a vitamin produced in the kernel. You will
use a gene that encodes a protein from a daffodil and you want the gene
expressed at a high level in the rice seed. Place the following steps in the
correct order to genetically engineer t
he sorghum.



1. Insert the gene into a single cell of a rice plant.


2. Extract DNA from a daffodil plant


3. Clone the gene for daffodil vitamin synthesis

4. Design the gene to express itself at a high level in the seed while the
rice grain is de
veloping

5. Breed a transgenic vitamin producing rice plant with a disease
-

resistant rice plant.

List these steps in their proper order:_____________________________


_____4. For genetic engineering in plants to be successful, where should
scienti
sts be focusing their transformation efforts?



a.) a single plant cell


b.) every cell of one seed


c.) every cell in one plant


d.) the leaves of the plant


View the “
Agrobacterium
” link and work through this animation.


5. List these steps in their proper order


____.
Put leaf disks from tomato in the dish with the agrobacterium


____.

Combine the Bt gene with the Ti plasmid (circle of DNA) vector


____. Transfer the tomato lea
f disks into a Petri dish with plant cloning media


____. Clone a transgenic tomato plant that has herbicide resistance as a
selectable trait plus insect resistance from the Bt gene.


____. Apply herbicide to the plant cells as they are growing in the
plant cloning
media


____. Introduce the Ti plasmid with the Bt gene into agrobacterium.


____ Place the agrobacterium into media plates where they can infect plant cells.



6. View the animation
Gene Coding
. Then your group should do the interactive
animation called “
Transgene Design
”.
Answer the following questions on the
transgen
e design animation.


a. transgene #1 Event 176 Promoter from gene______________________



Coding region from gene__________________


b. transgene #2 Mon810 Promoter from gene______________________



and Bt11


Coding region from gene__________________


c. transgene #3 Mon863 Promoter from gene______________________



Coding region from gene__________
________


d. transgene #1 Herculex Promoter from gene______________________



Coding region from gene__________________


7. . What is a selectable marker and how is this used in genetic engineering?





8.

Describe two ways that Animal Genetic engineering is different than Plant
Genetic engineering.





9.

Describe two steps that would be the same in Animal Genetic Engineering
and Plant Genetic Engineering.





10.

Rank these five animals from easiest to geneticall
y engineer (1) to most
difficult (4). Ties are not allowed.




___ elephant ___ salmon ___ mouse ___ cat ___ fruit fly



Describe your reasoning.





11.

What type of animal transformation is most likely to give chimeric animals
(ani
mals with some genetically engineered cells and some that are not)


___ pronuclear injection ___ blastocyst embryonic cell transfection




12.

What type of animal transformation does not allow the animal genetic
engineer to practice some sort of cell
level selection?


___ pronuclear injection ___ blastocyst embryonic cell transfection



13.

Discussion Board question:


a.
Animal or Microbe Genetic engineering project design.

Draw a diagram below
that outlines the five major steps in a genetic engineering project. Your project
should be on an animal or bacteria that you would like to genetically transform so
that it contains a transgene which improves a trait in the organism

or introduces a
new trait to the organism.



























b.
Share your genetic engineering plan with your group and then select one
project for your group. Improve and perfect the idea. Make a “poster” of your
group’s idea and we will display

this poster and use it in future problem sets.
Your poster should include
...


a.

The motive for doing this is explained

b.

All steps clearly shown including gene modification details and the
transformation method.

c.

A timeline for the project.







14
. Iron for
tified transgenic plants!!


Assume you are a genetic engineer and want to make a transgenic plant that will
have more iron (Fe) in the part of the plant that people eat.


a. Choose the plant that will be your Fe fortified food:


__ carrot

___ potato ___ wheat ___ soybean ___
cantaloupe



b. Assume that you have patent rights to the following genes. Chose the gene
that will be useful for your project


___ root symplast gene: turned on in root cells


___ turnip starch synthetase

gene: turned on in a turnip tap root


___ barley seed development gene: turned on in young seed cells of barley


___pumpkin fruit formation gene: turned on in a growing
pumpkin


___
watermelon

stem transporter: turned on in cells that ‘feed the phloem’.


___ pea cotyledon storage gene: turned on in pea cotyledons.


c. Explain why you chose the gene above.





d. Draw a diagram that describe your Fe Fortified Food GE project.
















15
.
Which picture is the best depiction of a chromosome in the
process of DNA
replication?





16
. Complete the drawing of DNA replication by labeling the following in addition
to the three enzyme listed below.


Replication fork, leading strand, lagging strand, DNA polymerase I, ligase