Project Lead The Way, Inc.
Genetic Engineering: Making E.Coli Glow Like Jellyfish
What are the ethical considerations involved in genetic engineering?
One question that is continually asked about the ethics of genetic engineering is
whether or not humans should be allowed to do this, and be able to change how life
essentially works and exists.
What are some beneficial applications of recombinant DNA technology?
Recombinant DNA allows us to combine desirable genes of one organism with another,
allowing us to create a hybrid with those genes, such as corn with more du
HB 101 a good candidate for a bioreactor?
This strain of E. Coli is easily readymade for recombination, and is easy to use when it
comes to cloning DNA in it. It is also sensitive to multiple antibiotics such as ampicillin
n seeing whether or not a recombination was successful.
What genes are found on the pGlo plasmid and what are there functions?
The Bla, araC, and GFP genes are found in this plasmid. Bla codes for resistance to
many antibiotics, including penicillin and
ampicillin. araC codes for the metablizing of
arabinose as a regulator of the GFP gene. GFP codes for the actual glowing of the
How does a genetic engineer distinguish bacteria containing the plasmid DNA
from those bacteria lacking the plasmid DN
First of all, the bacteria would be unable to grow in an environment containing the
ampicillin, and it wouldn’t glow under an ultraviolet light.
How does a genetic engineer determine if the bacterium has truly been
He is able to see the bac
teria grow in the ampicillin environment along with the GFP
gene allowing the bacteria to glow under an ultraviolet light after metabolizing
How does heat shock assist in gene insertion?
It doesn’t necessarily dissolve the cell membrane, but it
weakens it, or makes it more
porous allowing it to take up the plasmid allowing for the gene to be inserted into the
How does electroporation assist in gene insertion?
It uses an electric field to make the cell membrane and wall more porous to allow
easier insertion of a gene into the bacteria. This method is actually 10 times more
effective than chemical transformation.