What is Willy Wonka famous for?

whooshdisguisingΒιοτεχνολογία

14 Δεκ 2012 (πριν από 4 χρόνια και 8 μήνες)

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What is Willy Wonka famous
for?

Can you remember any of the
imaginative technologies Willy
Wonka created?

Who worked for him?


Oompa Loompas


They’re reaching
retirement age!


Willy Wonka is looking into
new technologies to produce
candy.


One technology
he’s working with
is genetic
engineering.


Goal: Learn how
genetic
engineering works
and apply the
technology to
other situations.

What is Genetic Engineering?


Process where
DNA

from one species is
transferred into DNA of another species.


The new strand (with DNA from both
species) is called
recombinant DNA
.



Bacteria

Willy Wonka’s Genetic
Engineering Experiment


He wanted to make large
quantities of Choco
-
M, a
protein

that is sweet and
chocolaty.


How did he do it?


In five steps!

I. Genetically Engineer a
Plasmid


5 Steps


Step 1: Remove DNA from the
Choco Plant cell

Remove DNA


Step 2: Remove plasmid DNA from
bacteria cell

Bacteria

Plasmid
DNA

Remove Plasmid DNA


Step 3: Cut both with a restriction
enzyme.

a.
Cut out Cocoa
-
M gene


b.
Cut plasmid with the same restriction
enzyme


c. These cuts make “
sticky ends
” (unparied
bases at the ends of the strands).


Step 4: “Glue” the sticky ends of the
gene to the sticky ends of the plasmid
with another enzyme called ligase.

Ligase


Step 5: Put recombinant plasmid back
into bacteria. Now this bacteria is
genetically engineered!

Bacteria

Production of Choco
-
M


Do people want to eat bacteria and
chocolate mixed together?


What does Willy Wonka want to do
now?



Make
a lot

of product


Have a
pure form

of chocolate

II. Amplify


Amplify the bacteria: this is making
clones
.


Bacteria is producing Choco
-
M protein
(and other bacterial proteins, too)

Clones

III. Isolate and Purify


Break open bacterial cells with
lysozyme
.


We only want Choco
-
M protein.


Right now it’s mixed with other bacterial
proteins.


Imagination


How could we quickly and efficiently
separate the 2 kinds of proteins?


Keep in mind

thousands of proteins
will need to be separated every hour!


Use tools from the big toolbox…or your
imagination!


How did you separate the two proteins?


What
property of the protein

did you
use?

Real
-
life Example: Insulin


Diabetics need insulin to help regulate
blood sugar.


Can you design a protocol for making
HUMAN INSULIN

in bacteria cells?