Genetic Engineering of Glucose/Xylose Co-fermenting - NREL

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11 Δεκ 2012 (πριν από 4 χρόνια και 4 μήνες)

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Poster Presentation 5
-
26


Genetic Engineering of Glucose/Xylose Co
-
fermenting
Saccharomyces
Yeast for Co
-
production of Ethanol and Various Industrial Enzymes


Miroslav Sedlak
1
, Zhengdao Chen
1
, Yanfang Pang
2
, Todd Applegate
2
,

Nancy Y.W. Ho
1*


1
Laboratory of

Renewable Resources Engineering (LORRE), Purdue University,

1295 Potter Center, West Lafayette, Indiana 47907


2

Department of Animal Sciences, Purdue University

Phone and Fax: 765
-
494
-
7046; E
-
mail:
nwyho@ecn.pu
rdue.edu



We should strive to make the cost for the production of cellulosic ethanol as low as
possible. One way to reduce the overall cost for the production of cellulosic ethanol is to
produce high valued co
-
products or by
-
products during the producti
on of ethanol. One
class of co
-
products could be various industrial enzymes that are high priced products.


One important industrial enzyme is phytase, which is used as a supplement in animal feed
to improve phosphorus nutrition and to reduce phosphorus p
ollution of animal excreta.


Saccharomyces

yeast has the GRAS status and has been used for the preparation of food
and drinks for human consumption for thousands of years. Thus, it can be used for the
production of any enzyme or special protein including
those for human and animal
consumption. In this presentation we focus on the expression and secretion of a bacterial
phytase in our glucose/xylose co
-
fermenting
Saccharomyces

yeast.