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Introduction to Cell Culture (CC)


BIOL454LP

Fall, 2010


LAB PRACTICAL GUIDE


Your lab practical exam score is based on effective use of sterile technique, coordination, timeliness,
and cleanliness in carrying out the basic techniques involved in cell cul
ture maintenance and passage.
To help us follow along as you perform the procedures below, we will ask you to tell us what you are
doing and why at each “step” in the process.



1.

Prepare the hood for use (excluding the UV sterilization step).


2.

Place any req
uired items into the hood for use.


3.

Determine the confluency of your plate of cells.


4.

Refeed your plate of cells and return the plate to the incubator.


5.

Split your cells to a new plate (details TBA).


6.

Put your new cell plate into the incubator.


7.

Clean up f
or the next person.


8.

Tell us at what confluency you expect your new plate of cells to be tomorrow. Explain
any assumptions involved in your estimation.



We expect all t
he procedures outlined above can be completed within
20 minutes
.


You will not be allow
ed to go over 25 minutes. Manage your time wisely.


LAB PRACTICAL SCHEDULE:

Start
Time
*

Student 1

Student 2

1:30

John S

Greg

1:55

Alex

Jose

2:20

Claire

Arash

2:45

Elizabeth

Jihyun

3:10

Daisy

Joana

3:35

Baron

Natalie

4:00

Abel

James


*Be prepared to

start early
. Arrive at the start time for the students who precede you. W
ait
outside the classroom until called in by the instructors. When you are finished with your
practical exam, you are free to go.