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Biosafety

Plan

Template


Introduction

Under the University of Saskatchewan
Biosafety Code of Practice
, appropriate safety measures
must be put into place by biosafety permit holders to
minimize

health and safety risks from the
use, storage, and disposal of biohazardous material.


This template is intended to assist biosafety permit applicants and permit holders develop a
biosafety plan that is in compliance with the
Occupational Health a
nd Safety Regulations
, the
Public Health Agency of Canada (PHAC)
Laboratory Biosafety Guidelines
, and the Canadian

Food Inspection Agency (CFIA) c
ontainment standards for f
acilities
.


A biosafety plan is required for all risk group (1, 2 and 3) organisms,
biological materials, or
biohazardous materials.


The biosafety plan must be available to all authorized workers and should form part of their
health and safety training under the biosafety permit.


The biosafety plan should be reviewed regularly, and upd
ated accordingly to reflect changes in
the work environment, new identified hazards, as well as changes to the biosafety permit.


General Instructions


Complete each section of the biosafety plan template.
The biosafety plan

should be
commensurate with risk group and containment level requirements for the organisms, biological
materials, or biohazardous materials to be used.

The biosafety plan should address all
organism(s), biological materials and biohazardous materials und
er the biosafety permit.
Do not
create a

separate biosafety plan for each type of permitted material.


Refer to the resources included in each section of the template for further information and to
assist in the development of the biosafety plan. All proce
dures, documents, templates, and
forms referenced in this procedure are in
italics
, and can be found on the Workplace Safety and
Environmental Protection (WSEP) website,
www.usask.ca/wsep
.


Upon completion of each
section of the biosafety plan, remove the instructions. Sample
biosafety plans are provided in Appendix A of this template. For assistance preparing a
biosafety plan, contact the Bio
safety Manager, WSEP at 966
-
8496 or the Biosafety Consultant
at 966
-
8483
.


R
emove instruc
tions upon completion of section.







Biosafety Plan


Year



College
/Unit
/Department



Biosafety Permit Number:

i


Contents


1

Biosafety Permit
Information

................................
................................
...............................

1

2

Nature of Research Work

................................
................................
................................
....

2

3

Risk Assessment

................................
................................
................................
................

3

4

Work Locations

................................
................................
................................
...................

6

5

Health and Safety Hazards

................................
................................
................................
.

8

6

Health and Safety Control Measures

................................
................................
..................
10

7

Biosecurity

................................
................................
................................
.........................
11

8

Hazardous Waste Disposal

................................
................................
................................
13

9

Emergency Response

................................
................................
................................
........
14

9.1

Medical Incidents

................................
................................
................................
........
14

9.1.1

Medical Incidents Involving Exposure to a Biohazardous Material

.......................
14

9.1.2

Other Medical Incidents

................................
................................
.......................
15

9.2

Spills of Hazardous Material

................................
................................
.......................
16

9.2.1

Chemical Spills

................................
................................
................................
....
16

9.2.2

Biological Spills

................................
................................
................................
....
17

9.3

Safety Equipment Failure

................................
................................
............................
18

9.4

Loss or Theft of Biohazardous Materials

................................
................................
.....
18

9.5

Power Outages

................................
................................
................................
...........
19

9.6

Fire

................................
................................
................................
.............................
20

10

Training

................................
................................
................................
..........................
21



ii


Revision History


Revisions to th
e biosafety plan ar
e documented in Table 1, Revision History.


Table 1: Revision History

Document
Section

Details of Amendments

Date

Author

(Initials)


























Biosafety Plan


Date

College/Unit/Department


Page
1

of
25


1

Biosafety Permit Information


Biosafety Permit Number:

Biosafety Permit Issue Date:


Permit Holder Name:

Permit Holder Telephone Number:

Email:


Laboratory Contact Name:

Laboratory Contact Telephone Number:

Email:





Biosafety Plan


Date

College/Unit/Department


Page
2

of
25


2

Nature of
Research
Work


Instructions:


Provide a description of the nature of the work that will be performed in the re
search protocol and under
your biosafety p
ermit. Reference any relevant documents.

Include relevant reference information in an
appendix of the biosafety plan.


R
emove instruc
tions upon completion of section.


ENTER TEXT HER
E….




Biosafety Plan


Date

College/Unit/Department


Page
3

of
25


3

Risk Assessment


Instructions
:


The

critical step in developing a biosafety plan is to determine the risk group and containment level for the
organism, biological material or biohazardous material that is to be used.

Listed below, are a number of
resources that provide risk group and contai
nment lev
el information for a wide range of

organisms and
biohazardous materials. These resources are assessable on the WSEP website,
www.usask.ca/wsep
.




Human Pathogens and Toxins Act,

(
http://www.phac
-
aspc.gc.ca/lab
-
bio/regul/index
-
eng.php
).



Pathogen Safety Data Sheets and Risk Assessment,
P
HAC
,

(
http://www.phac
-
aspc.gc.ca/lab
-
bio/res/psds
-
ftss/index
-
eng.php
).



Disease Agent Information,
C
FIA
,
(
http://www.inspection.gc.ca/english/sci/bio/anima/disemala/d
isemalae.shtml
)
.



Risk Group Classification for Infectious Agents,
America
n Biological Safety Association
,

(
http://www.absa.org/riskgroups/index.html
).



National Institutes of Health Guidelines for R
esearch Involving Recombinant DNA Molecules,
Nat
ional Institutes of Health

(NIH)
,

(
http://oba.od.nih.gov/oba/rac/Guidelines/APPENDIX_B.htm#_Toc7238342
).



Biosafety in M
icrobiological and Biomedical Laboratories
, Centers for Disease Control,
National Institutes of Health,

(
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
).


WSEP also
maintains a database of organisms, biological material
s

and biohazardous material
s

that are,
or have been used at the university together with their identif
i
ed risk group and containment level.


If the risk group and containment level of the o
rganism
,
biological mate
rial or biohazardous material has
been determined by
an
external source
(s)

(e.g. PHAC, CFIA
, NIH
), reference

the source and available
technical information.


If the organism, biological material or biohazardous material risk group

and contai
nment level

is not
already determined, a biological risk group and containment level assessment must be performed. The
Biological Risk Group and Containment Level Assessment

Template

is to be used to determine the
appropriate risk group and corresponding c
ontainment level of each organism, biological material or
biohazardous material.

Include a copy of each

biological risk group and containment level assessment

for
each permitted material

as an appendix
to the biosafety plan.


If the organism is a recombina
nt DNA (rDNA), a genetically modified microorganism(s), or a transgenic
organism(s), a biological risk group and cont
ai
nment level assessment must

be

conducted

even if the risk
group has already been determined

by an external source
.

Refer to t
he
Biologica
l Risk Group and
Containment Level Assessment

Template

for what sections to complete
.

Include a copy of each biological
risk group and containment level assessment for each permitted material as an appendix to the biosafety
plan.


Include or reference
available technical information including Material Safety Data Sheets (MSDS),
technical data sheets and/or related journal articles for each organism, biological material or
biohazardous material.



Biosafety Plan


Date

College/Unit/Department


Page
4

of
25


Prepare an inventory of the organisms, biological material
s and biohazardous materia
ls that will be used
under the biosafety p
ermit (
see
Table 1).


R
emove instruc
tions upon completion of section.


Biosafety Plan



Date

College/Unit/Department



Page
5

of
25


Table 1:
Inventory of organisms, biological materials and biohazardous materials.

Name

of Biological Material

Purpose or Use

Quantity/Concentration

Risk
Group*

(1, 2, 3)

Containment
Level*

(1, 2, 3)

References
**























































*The identified risk group

and containment level

as

specified by external sources,

or as determined from a biological risk and containment level assessment.

**Indicate the reference for the categorization of the risk group and containment level (e.g.
r
isk assessment performed by researcher, PHAC, CFIA).



Biosafety Plan


Date

College/Unit/Department


Page
6

of
25

4

Work Locations


Instructions:


Identify the
locations where the organism(s), biological material
s

and biohazardous material
s

will be used
and stored. Include a description of the facility and safety equipment (e.g. biosafety cabinets, autoclaves)
that wil
l be employed.

Complete Table 2.


Work and storage locations must meet containment level requirements as determined from the risk
assessment information for each organism, biological material or biohazardous material. T
he following
resources provide
details of facility design and operational requirements based on the identified
containment level.




Laboratory Biosafety Guidelines
, PHAC,

(
http://www.phac
-
aspc.gc.ca/lab
-
bio/
res/blk
-
acb/lbg
-
ldmbl
-
eng.php
).

Containment Standards for Veterinary Facilities
, CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/convet/convete.pdf
).



Containment
Standards for Facilities Handling Plan Pests
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/plaveg/placone.pdf
).



Containment Standards for Facilities Handling Aquatic Anima
l Pathogens
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/aqu/csfncie.pdf
).



Laboratory Biosafety Manual
, W
orld Health Organization
,

(
http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/
).



Biosafety in Microbiological and Biomedical Laboratories
, Centers for Disease Control, National
Institutes of Health,

(
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
).


The Biosafety Manager is available to assist with ensuring facilities meet containment level standar
ds.


R
emove instruc
tions upon completion of section.



ENTER TEXT HERE….



Biosafety Plan


Date

College/Unit/
Department


Page
7

of
25

Table 2: Biosafety p
ermit w
ork and storage locations.

Location

Purpose
*

Nature of
Work

Safety Equipment*
*

Containment
Level**
*

Type and Model

Serial Number





































*Provide the purpose or use of the location which could include, work, diagnostic, decontamination, storage, etc..

*
*
Safety equipment
include
s

but is not limited to, biosafety cabinets, fume hoods, hand washing sinks, emergency eyewash

and shower equipment
.

**
*
Depending on the required containment level, there will be specific facility location, access, design and construction, air h
andl
ing, containment perimeter,
laboratory services, and safety equipment. Refer to the Public Health Agency of Canada (PHAC
) Laboratory Biosafety Guidelines

and

Canadian Food Inspection
Agency (CFIA)
Containment Standards
.




Biosafety Plan


Date

College/Unit/Department


Page
8

of
2
2


5

Health and Safety Hazards


Instructions:


To dete
rmine
health and safety risks
, including risks of exposure to pathogens and toxins
, one must
consider not only the types of biohazardous
materials being used but also the

proposed

manipulation
s

and handling of these materials

under the research protocol or activity
.


For each organism, biological material or biohazardous material, or class of materials

(with similar risk
characteristics)
, c
omplete the following sections under
Health and Safety Hazards
.


1.

Name of biological material
, its risk group and required containment level.


2.

Identify each procedure/technique that will be carried out

under the permit.

Include equipment
and tools that will be used.


3.

I
dentify the exposure hazards (
risk groups 2 and 3 only
) and any other health and safety
hazards

(e.g. chemical, radiological, physical) that exist

for each procedure/technique
.


Consider the key steps in
each procedure/technique when identifying health and safety hazards.


The following
procedures
commonly
used in the laboratory may create a risk of exposure to

organisms or

biohazardous material.




Phlebotomy
techniques on

humans or animals
;



Injections into

humans or animals using animal or human specimens
;



Other use of needles with human or animals
;



Handling human or animal tissue, including preparation, dissection, cutting
;



Pipetting, mixing, or vortexing human or animal blood, fluid, or tissue
;



Pipetting,

mixing
, or vortexing biohazardous material;



Centrifuging human
or animal blood, fluid, tissue,

organisms or biohazardous material;



Handling tubes or other container or human or animal blood, fluid, or tissue

or
biohazardous material;



Preparing or handling

h
uman, animal or biohazardous material

cell cultures
;



Handling contaminated sharps or other contaminated waste
; and



Cleaning spills of human or
animal

blood or other body fluids

or biological hazardous
material.


4.

Identify the possible diseases o
r adverse
health effects

associated with exposure to the or
ganism
or biohazardous material

(
risk group
s
2 and 3 only
)
. Include the
signs and
symptoms of known
diseases.


5.

Identify the e
xposure routes for the organism or biohazardous material (inhalation, ingestion,
skin
contact, injection)
.


6.

Provide a description of known allergies that could develop from working with

the

organism
(
s
)

(
risk groups 1, 2 and 3
)
. Include symptoms of the allergies.






Biosafety Plan


Date

College/Unit/Department


Page
9

of
2
2


Resou
r
ces


Following are resources that provide
information on biosafety
.




Laboratory Biosafety Guidelines
, PHAC,

(
http://www.phac
-
aspc.gc.ca/lab
-
bio/res/blk
-
acb/lbg
-
ldmbl
-
eng.php
).

Containment Standards for Veterinary Facilities
, CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/convet/convete.pdf
).



Containment Standards for Facilities Handling Plan Pests
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/plaveg/placone.pdf
).



Containment Standards for Facilities Handling Aquatic Animal Pathogens
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/aqu/csfncie.pdf
).



Laboratory Biosafety Manual
, W
orld Health Organization,

(
http://www.who.int/
csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/
).



Biosafety in Microbiological and Biomedical Laboratories
, Centers for Disease Control, National
Institutes of Health,

(
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
).


R
emove instruc
tions upon completion of section.


ENTER TEXT HERE….




Biosafety Plan


Date

College/Unit/Department


Page
10

of
2
2


6

Health and
Safety

Control

Measures


Instructions:



Describe the safety measures that are in place
to protect workers from
exposure

to biohazards,

or other
identified hazards
. Safety measures
should be commensurate with the identified hazards and
may
include:




Engineering controls

(i.e. facility design, biosafety cabinets
, fume hoods, chemical storage
)
;



Work p
rocedures and
rules;



Pers
onal protective equipment (PPE);



Medical surveillance (medical examination, v
accinations if available and recommended
, serum
screening)
;


In this section,
also
o
utline the metho
ds and procedures to be used
for
the
d
isinfection and
decontamination of work areas, equipment and materials.


Resou
r
ces


Following are resources that provide information on biosafety.




Laboratory Biosafety Guidelines
, PHAC,

(
htt
p://www.phac
-
aspc.gc.ca/lab
-
bio/res/blk
-
acb/lbg
-
ldmbl
-
eng.php
).

Containment Standards for Veterinary Facilities
, CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/convet/convete.pdf
).



Containment Standards for Facilities Handling Plan Pests
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/b
io/plaveg/placone.pdf
).



Containment Standards for Facilities Handling Aquatic Animal Pathogens
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/aqu/csfncie.pdf
).



Laboratory Biosafety Manual
, W
orld Health Organization,

(
http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/
).



Biosafety in
Microbiological and Biomedical Laboratories
, Centers for Disease Control, National
Institutes of Health,

(
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
).


R
emove instruc
tions upon completion of section.


ENTER TEXT HERE….






Biosafety Plan


Date

College/Unit/Department


Page
11

of
2
2


7

Biosecurity


Instructions:



The permit holder shall implement and maintain biosecurity measures co
mmensurate with the identified
risk group and containment l
evel of the biohazardous materials under
the biosafety permit and legislative
requirements. This includes, but is not limited to:




Physical protection of the permitted facility to minimize unauthorized access to facilities,
laboratories and storage areas;



Personnel authorization and clearance of
authorized workers to work in the facility;

and



Inventory management of organisms, biological materials, and biohazardous materials used and
stored

under the biosafety permit.


Incident reporting, response, and investigation into suspected criminal activit
y including the loss or
suspected theft of organisms, biological materials, or biohazardous materials
is
addressed

under Section
10
,

Emergency Response
.


In the section, provide a description of
the biosecurity measures for the facility being permitted. Th
e
minimum requirements for biosecurity for risk group 1 and 2 are:




Controlled access to the facility.
Doors to facilities should be kept closed at all times. Access
doors to the facility should be lockable.



Access to the facility should be limited to
auth
orized workers
. Visitors must be accompanied by
an authorized worker.



An up
-
to
-
date i
nventory of organisms, biological materials, and biohazardous materials used and
stored

under the biosafety permit.


For the use of risk group 3 biohazardous materials, a
biosecurity risk assessment is to be performed
. The
assessment should include an examination of

possible threats and vulnerabilities, and the
countermeasures or mitigation strategies specific
to the

facility. Contact t
he Biosafety Manager at 966
-
8496 or th
e Biosafety Consultant at 966
-
8483

for
more information and assistance.


Resou
r
ces


Following are resources that
provide information on biosecurity
.




Laboratory Biosafety Guidelines
, PHAC,

(
http://www.phac
-
aspc.gc.ca/lab
-
bio/res/blk
-
acb/lbg
-
ldmbl
-
eng.php
).

Containment Standards for Veterinary Facilities
, CFIA,

(
http://www.inspection.gc.ca/engli
sh/sci/bio/anima/convet/convete.pdf
).



Containment Standards for Facilities Handling Plan Pests
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/plaveg/placone.pdf
).



Containment Standards for Facilities Handling Aquatic Animal Pathogens
,
CFIA,

(
http://www.inspection.gc.ca/english/sci/bio/anima/aqu/csfncie.pdf
).



Laboratory Biosafety Manual
, W
orld Health Organization,

(
http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_CSR_LYO_2004_11/en/
).



Biosafety in Microbiological and Biomedi
cal Laboratories
, Centers for Disease Control, National
Institutes of Health,




Biosafety Plan


Date

College/Unit/Department


Page
12

of
2
2


(
http://www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf
).


R
emove instruc
tions upon completion of
section.


ENTER TEXT HERE….






Biosafety Plan


Date

College/Unit/Department


Page
13

of
2
2


8

H
azardous Waste Disposal


H
azardous

waste (biohazardous or other types)

shall

be collected and packaged in accordance
with the WSEP
Hazardous Waste Disposal Manual

and applicable legislation.


Instructions:


Provide a
description of the disposal of hazardous and non
-
hazardous biological waste and other

hazardous

waste (
e.g. chemical, radiological) that

may be g
enerated during work activities under the
biosafety permit.

The method of disposal for each type of hazardous w
aste (e.g. chemical disinfection,
autoclave, service provider) should be included in the description.


Biohazardous waste is to be collected and packaged in accordance with the WSEP
Hazardous Waste
Disposal Manual
.


R
emove instruc
tions upon completion of
section.


ENTER TEXT HERE….






Biosafety Plan


Date

College/Unit/Department


Page
14

of
2
2


9

Emergency Response


Instructions:


Provide a description of th
e measures to be taken in the event of an emergency. Procedures should be
developed to respond to the following types of incidents:




Medical emergencies which
include injuries and confirmed or suspected illness from exposure to
hazardous materials;



Spills of hazardous materials;



Containment equipment failures;



Loss or theft of hazardous materials;



Power outages;



Fire; or



Other types of incidents related to the w
ork activities

(e.g. an uncontrolled release to the
environment)
.


The followin
g

sections may be used as is
or adjusted to suit

specific requirements.


R
emove instruc
tions upon completion of section.


9.1

Medical Incidents


9.1.1

Medical Incidents

Involving Exposure to a Biohazardous Material


When an authorized worker has been or
is
believed
to have been exposed to an organism or
biohazardous material,
the following steps are to be followed

by the individual exposed
.


1.

Remove any contaminated cloth
ing

and
personal
protective equipment.


2.

Flush contaminated

areas with

soap and

warm

water.

If eyes are exposed, flush eyes for
15 minutes using an emergency eyewash station.



If there is an open wound to the

skin (cuts, punctures),

encourage

bleeding
,

flu
sh
area
with water, apply an antiseptic, and cover with gauze.


3.

N
otify the

supervisor and the permit holder (if different

from the supervisor
).


4.

If prescribed, s
eek professional medical attention immediately.

Ensure to take any
applicable documents (e
.g. M
aterial Safety Data Sheets
, technical documents) related to
the organism or biohazardous material exposed to.


5.

C
omplete an incident report using the WSEP online incident reporting system
(
www.usask.ca/wsep
).







Biosafety Plan


Date

College/Unit/Department


Page
15

of
2
2


Following are the supervisor’s responsibilities when an incident occurs.


1.

Implement immediate corrective actions as required in response to the incident.


2.

If the individual involved in the incident had to seek professional medical attention,
contact Health

and Wellness to complete appropriate Saskatchewan Workers’
Compensation Board (WCB) forms.


3.

The incident is to be reviewed by the supervisor and WSEP. Appropriate
preventative
measures are to be developed, implemented,
and communicated

to staff to minimiz
e the
recurrence

a similar incident.


9.1.2

Other Medical Incidents


In the event of a medical incident, the following steps shall be followed.


1.

Seek medi
cal attention as required or deemed necessary.


2.

Notify the supervisor and the permit holder (if different
from the supervisor).


3.

Complete an incident report using the WSEP online incident reporting system
(
www.usask.ca/wsep
).


In the event of a medical emergency,


1.

Call for help (Campus Safety 5555 or
9
-
911).


2.

Provide
life saving first aid, if safe to do so.


3.

Do not transport the victim.


Following are the supervisor’s responsibilities when an incident occurs.


1.

Implement immediate corrective actions as required in response to the incident.


2.

If the individual involved in

the incident had to seek professional medical attention,
contact Health and Wellness to complete appropriate Saskatchewan Workers’
Compensation Board (WCB) forms.


3.

The incident is to be reviewed by the supervisor and WSEP. Appropriate preventative
measure
s are to be developed, implemented, and communicated to staff to minimize the
recurrence a similar incident.







Biosafety Plan


Date

College/Unit/Department


Page
16

of
2
2


9.2

Spills of Hazardous Material


9.2.1

Chemical Spills


A spill kit is located
specify location(s) within your facility
.


Emergency contact information i
s posted
specify location(s) within your facility
.


Only individuals trained in spill response shall respond to a spill.


In the event of a large spill
,

or

if the spill jeopardizes the health and safety of any person,
cordon
off the spill area, alert everyone in the area, and
contact WSEP at 966
-
4700

(after hours 5555)
.


If
you have

been injured as a result of a spill,
s
eek medical attention first

before addressing the
spill
.



Following are the general steps to be foll
owed in response to a chemical spill
:


1.

Assess the spill.

If you feel you
are
unable to deal with the spill yourself, seek help.


2.

Cordon off the area around the spill. Alert everyone

in the area that
a spill

has occurred
.



3.

Gather
the
spill kit. As
necessary,

refer to MSDS for the chemical(s)

spilled
.


4.

Do
n appropriate personal protective equipment

(
specify required personal protective
equipment
)
.


5.

Apply
an
appropriate
sorbent (absorbent/
adsorbent
)

to

the spill.
It is important that the
sorbent match
the chemical hazard and capture hazardous vapours if produced.
Give
the sorbent sufficient time to work.


6.

Using appropriate tools, g
a
ther the spilled material and
sorbent
,

and place it in an
appropriate
waste
container.

Always work from the outside of the

spill area towards the
centre of the spill. Repeat steps 4 and 5 as necessary.


7.

Label the
waste
container with its contents.



8.

Decontaminate the spill area
, equipment and tools used in the spill
. Generally, the area
may be cleaned with soap and water.


9.

Inform personnel in the area that the spill has been cleaned up.



10.

Report the spill to your supervisor and complete

an
Incident

Report on the WSEP
website (
www.usask.ca/wsep
)
.


11.

As necessary, restock the spill kit.




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2



9.2.2

Biological Spill
s


A spill kit is located
specify location(s) within your facility
.


Emergency contact information is posted
specify location(s) within your facility
.


Only individuals trained in spill response shall respond to a spill.


In the event of a
large spill, or if the spill jeopardizes the health and safety of any person, cordon
off the spill area, alert everyone in the area, and contact WSEP at 966
-
4700

(after hours 5555)
.


If you have been injured as a result of a spill, seek medical attention f
irst before addressing the
s
pill.


Following are the general steps to be followed in response to a spill

involving organism,
biological materials or biohazardous materials
:


1.

Assess the spill. If you feel you are unable to deal with the spill yourself, seek

help.


2.

Cordon off the area around the spill. Alert everyone in the area that a spill has occurred.


3.

Gather
the
spill kit. As necessary, refer to MSDS for the organism, biological materials or
biohazardous materials spilled.


4.

Do
n appropriate personal pro
tective equipment

(
specify required personal protective
equipment
)
.

If the biological material may be aerosolized, appropriate respiratory
protection may be required.


5.

Gently cover the spill with paper towels.


6.

Apply an appropriate
disinfectant
to
the pape
r towel (
list the disinfectant(s)

and
concentrations

that may be used
). Apply the disinfectant from the edges to the centre of
the spill
. G
ive the
disinfectant

sufficient time to work

(
specify the required contact time
prior to cleaning the spill area)
.


7.

Using appropriate tools, gather the spilled material, and place it in an appropriate

biohazard

waste container

or autoclave bag
. Always work from the outside of the spill
area towards the centre of the spill.

Use forceps, tongs, or a broom to remove broken

glass and other items.

Sharps should be place in an appropriate container.
Repeat steps
6

and
7

as necessary.


8.

Label the waste container with its contents.



9.

Decontaminate the spill area, equipment and tools used in the spill. Generally, the area
may be c
leaned with soap and water.




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10.

Decontaminate (autoclave/chemical treatment) reusable clean
-
up items and other
reusable equipment.


11.

Inform personnel in the area that the spill has been cleaned up.


12.

Report the spill to your supervisor and complete

an
Incident

Report on the WSEP
website (
www.usask.ca/wsep
).


13.

As necessary, restock the spill kit.


If a spill occurs in a biosafety cabinet, ensure
to

keep the biosafety cabinet running during the
spill cleanup. Ensure to wipe down all affected areas within the cabinet including the back and
side walls. Allow the biosafety cabinet to run for 10 minutes after the clean
-
up is complete
before resuming work

in the cabinet.


If a spill occurs involving a centrifuge, turn off the centrifuge and do not open the lid for 20
minutes to allow aerosols to settle.

Apply disinfectant to all contaminated surfaces. Remove
buckets and rotors to nearest biosafety cabinet
for disinfection.


9.3

Safety
Equipment Failure


The primary engineered safety

equipment utilized
when
handling and manipulating
biohazardous materials is the biosafety cabinet. In the event that a biosafety cabinet is

s
uspected of not working properly
, is a
la
rming
, or p
ast certification dat
e, work in the cabinet
should stop immediately.


Contact WSEP at 966
-
4700 for certification testing of all biosafety cabinets. Contact the
Facilities Management Division Work Control to schedule repairs or maintenance for th
e
biosafety cabinet

(Phone: 966
-
4496, Facsimile: 966
-
8854,
workcontrol.centre@usask.ca
).


9.4

Loss or Theft of Biohazardous Materials


In the event that biohazardous materials under a biosafety permit are susp
ected of being lost or
stolen, the following steps shall be followed.


1.

Verify the missing biohazardous materials against the inventory.


2.

Attempt to locate the missing biohazardous materials.


3.

If the materials cannot be located, immediately notify your
supervisor and the permit
holder (if different).


4.

Contact W
SEP (966
-
4700). WSEP will determine

if

other agencies need to be notified
.


5.

Complete

an
Incident

Report on the WSEP website (
www.usask.ca/wsep
)
.




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An investi
gation shall be conducted for all biohazardous materials suspected of being lost or
stolen. The investigation shall include a review of all biosecurity measures in place under the
biosafety permit.


9.5

Power Outages


Instructions:


Procedures specific to the
facility and the nature of the work being performed to safely address power
outages should be included in this section. Following is a general procedure for power outages.



If experimental animals are in use, special precautions may need to be taken to secure those areas such
as emergency power, alternate ventilation, etc..


Identify essential equipment in the facility/laboratory which requires emergency power. Contact the
F
acilities Management Division Work Control to provide emergency power to equipment (Phone: 966
-
4496, Facsimile: 966
-
8854,
workcontrol.centre@usask.ca
).


Although refrigerators and freezers will maintain th
eir temperature for several hours if not opened, identify
an emergency source of dry ice if there are items that must be kept cold during extended power outages.
Dry ice should not be used in walk
-
in refrigerators or other confined areas because hazardous
concentrations of carbon dioxide will accumulate.


Ensure that no flammable chemicals are stored in domestic refrigerators and freezers. This may cause a
dangerous condition due to the generation of explosive or flammable vapours.


R
emove instruc
tions upon

completion of section.


All personnel should receive training on the steps to be followed in the event of a power outage.


Emergency contact information is posted
specify location(s) within your facility
.


All facilities
are

eq
uipped with emergency
lighting.



A

log

shall be maintained

of equipment

in the facility

that must be reset, restarted or
which
require special attention.

Equipment that operates unattended should be programmed to shut
down safely and not restart when power returns.

It is good
practice to turn off all non
-
essential
equipment when not in use.



In the event of a power outage, the following steps shall be followed.


1.

Immediately discontinue all work in fume hoo
ds or biosafety cabinets.


2.

Cap all open containers of volatile solution
s
, biological materials, and biohazardous
materials
.





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2


3.

Secure current experiment work according to specified procedures (
specific procedures
to be followed relating to an experiment, if there is a power outage
). Shut down
experiments that involve hazardous
materials.


4.

Turn off
all non
-
essential
equipment
(e.g.
centrifuges, stirrers, hotplates
) to prevent
tripping breakers, blowing fuses, or damaging equipment when power is returned.


5.

Turn off all
gas cylinders at the tank valves.


6.

Close all laboratory refrigerators and freezers and avoid opening them. Do not unplug
them.


7.

If an emergency exists or if

directed, evacuate the facility.


When the power returns to the facility, the following steps shall be followed.


1.

Check for any stran
ge odours or spills. Contact WSEP (966
-
4700).


2.

Reset or restart equipment as necessary.


3.

Check airflow in biosafety cabinets and fume hoods.

Allow biosafety cabinets and fume
hoods to operate for at least 10 minutes before resuming work.

If they appear not

to be
functioning, contact Facilities Management Work Control (Phone: 966
-
4496, Facsimile:
966
-
8854,
workcontrol.centre@usask.ca
)
, or WSEP (966
-
4700)
.


4.

Che
ck refrigerators and freezers to ensure they are
operating.


9.6

Fire


In the event of a fire, the following steps shall be followed.


1.

Assess the severity of the fire. If you can do so safely, extinguish the fire using an
appropriate fire extinguisher.


2.

Notify individuals in the area that a fire occurred. As necessary, evacuate the area.


3.

Immediately report the fire to your supervisor, the permit holder (if different), and to
WSEP (966
-
4700).


4.

Complete

an
Incident

Report on the WSEP website (
www.usask.ca/wsep
).


If you cannot safely extinguish the fire, pull the nearest fire alarm and evacuate the building in
accordance with building’s evacuation plan. Call 5555 for emergency assistance.







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10

Training


Instructions
:


The following section may be used as provided. Include any additional training that may be required to
perform work activities.


Resources


WSEP courses may be registered for directly through its website,
www.usask.ca/wsep
. Additional training
may also be required depending on the nature of the work being performed. Contact WSEP for further
information on training requirements and available courses.


Remove instructions upon completion of section.



Author
ized w
orkers

listed under the biosafety permit

must complete the following training
offered by WSEP:




Employee Safety Orientation;



Biosafety Course;



List other training that

is required (e.g. Labor
atory Safety, Workplace Hazardous
Materials Information Sy
stem (WHMIS)
);



……


Authorized w
orkers
shall receive site
-
specific training from

the permit holder, or designate.
Site
-
specific training shall

include information on identified

hazards in the work environment and
what safety measures are to be followed to
protect workers. Instruction should also include the
procedures to be followed in the event of an incident or an emergency.


All authorized w
orkers must read and understand the
biosafety plan

prepared under the
biosafety p
ermit.


An
Authorized Worker
Biosafety Training Record

form shall be completed for each authorized
worker.


R
ecords of all training are maintained by the permit h
older.