Bioinformatics Program – Bruce Luxon, Ph - UTMB Research ...


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Core Program

(Link to Brief Description)

Web Page Link

Director or Manager


Bioinformatics Program

Bruce Luxon, PhD

Dean of

Biomedical Imaging Network

Massoud Motamedi

Microbiology &

Optical Microscopy Core


Leoncio Vergara,
Adrianna Paulucii

Biomolecular Resource


Alex Kurosky, PhD

Dean of

Protein Chemistry Section

Alex Kurosky, PhD

Biosynthesis & Biomarker

Bo Xu, PhD


Peptide Synthesis Core

Stefan Serabyn


Protein Chemis
try Core

J. Stephen Smith


Proteomics Section

John Wiktorowicz, PhD




John Wiktorowicz, PhD


Proteomics Bioinformatics

Kizhake Soman
, PhD


Mass Spectrometry Core

Tony Haag, PhD



Dan Freeman,

Medicine &
Health (PMCH)

Environmental Exposure

Bill Ameredes, PhD

Sealy Center for
Health &

Flow Cytometry and Cell

Jiaren Sun
, PhD

Microbiology &

Institute for Translational
Research (ITS)

Allan Brasier

ITS Clinical Research Center
and Imaging Laboratory

Melinda Sheffield
Moore, PhD

Thomas Wood, PhD

Research Histopathology

Judy Aronson, MD


Research Technology


Jamie Svrcek


Sealy Center for Cancer Cell
Biology (SCCCB)

Robert Ullrich, PhD


Transgenic Mouse Facility

Maki Wakamiya,



Nude Mouse Laboratory

Mark Hellmich,


Real Time PCR Core


Lisa Elferin
k, PhD

Nonggao He, PhD


UTMB Tumor Bank


Nonggao He, PhD


Biostatistics Shared Service


Cancer Center

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Core Program

(Link to Brief Description)

Web Page Link

Director or Manager


The Oncology Clinical Trials
Office (OCTO)

vi Markowitz
, MD


Cheryl Proctor (Mgr)

Cancer Center


in vivo

Core (RIVA)


Kelly Dineley
, PhD


Sealy Center for Molecular
Medicine Cores

Allan Brasier, MD


Molecular Genomics

Thomas Wood, PhD

Sealy Center
for Molecular

Recombinant DNA Lab

Thomas Wood, PhD

Sealy Center
for Molecular

Sealy Center for Structural
Biology (SCSB) Cores


Wayne Bolen,
Ph.D., Director

Dean of

Computational Biology

Wayne Bolen, PhD

David Power, Manager


Cryo Electron Microscopy

Michael Sherman, PhD


Solution Biophysics


Luis Holthauzen, PhD


NMR Spectroscopy

Tianzhi Wang, PhD


ray Crystallography

Mark White, PhD


Synthetic Organic Chemistry

Richard Hodge, PhD

aly Center for
Health and

Tissue Culture Core Facility

Rolf Konig, PhD



Galveston National

James W. LeDuc,





ohnny Peterson,


Assay Development


Nigel Bourne, PhD


Experimental Pathology


David Walker,




Massoud Motamedi,
PhD (acting





Insectary Services


Stephen Higgs, PhD


Preclinical Studies


Slobodan Paessler,


Regulatory Services


David Bea
sley, PhD


Shope BSL 4 Core Lab

Alexander Freiberg,


Sealy Center for
Environmental Health &

Istvan Boldogh,
&B, PhD

NIEHS Center

Pepper Center Cores
Elena Volpi, MD,
Sealy Center on

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PhD; James S.
Goodwin, MD


Tracking and

Jean Freeman, PhD

Pepper Center

OAIC Administrator

Tony DiNuzzo, PhD

Pepper Center

Clinical Research
Resource Core

Glenn Ostir, PhD

Leader: Elizabeth
Protas, PhD, PT

Pepper Center

Muscle Biology Resource

Elena Volpi, MD,
PhD; Co
Leader John

er Center

Pilot/Exploratory Studies

Blake Rasmussen
PhD; Co
Melinda Sheffield
Moore, PhD and
James Goodwin, MD

Pepper Center

Research and Career
Development Core

Ken Ottenbacher,
hD; Co
Kyriakos Markides,
PhD and James
Goodwin, MD

Pepper Center

Resources: Core Facilities

The Biomedical Informatics Program

has labs and offices in the Dockside Building, the Basic Science Building,
Children’s Hospital, John Sealy Hospital, and the Medical Research Building. Staff includes the Program Directo
r (Prof.
Luxon), an administrative assistant, 7 faculty members (as well as their staff), 7 senior staff, and 5 regular and junior sta
We also have several casual BioIT Team members drawn from the institutional IT force on an
ad hoc

basis when special
skills are required that are not otherwise available within the Team.

Our mission is to provide expertise, training, access to high
end hardware and software, and analytical support in BMI to
researchers. This includes high
level multivariate analysis, d
ata mining, developing analytical software and web
relational databases, and data management. Hardware, software, and web and data management support services are
provided through our BioIT Team. We collaborate with a wide range of scientific and cl
inical projects which use
genomics, all forms of proteomics, metabolomics, kinomics, and systems biology in their research. Our efforts are
strongly focused on systems biology and signaling pathways with a special emphasis on the incorporation of clinical

into these analyses for greater translational impact. The BMI Bio
IT Team is responsible for EMR data extractions and
the i2b2 operation at UTMB. We have also added a substantial research effort in network visualization for analysis of
biomedical d
ata, with translation of the results to the design of decision
support systems and improving the computer/user
interface. We are finished the development of our Next Generation Sequencing (NGS) bioinformatics support core which
will go into full service l
ate spring 2011.

We are exceptionally well
equipped for computationally demanding projects. We recently added a dedicated Dell Linux
cluster with 17 state
art Intel Xeon 5650 nodes giving a total of 192 processors with 408 GB RAM running the
at Linux OS. This substantially improves our computational support for NGS bioinformatics as well as all other
computationally intensive efforts. We also have a Sun Microsystems SunFire 4800 server with 12 UltraSparc III 1050MHz
CPU and 24GB RAM as our d
ata mining server, as well as a number of unix and PC workstations for data analysis. Most
BMI faculty and staff also have a high
end Dell laptop for their personal use. We have nearly 50TB of disk space
contained within a highly
scaleable storage area n
etwork system (SAN). The SAN is a level 5 RAID hot
swappable disk
system with a robotic tape backup. The SAN, the Oracle RDBMS, and web services are controlled by a SunFire V880
server with 4x1050MHz CPU and 8GB of RAM. We have virtually every kind of p
eripheral equipment necessary for state
of the art BMI operations. We use Splus as our primary statistics engine, MatLab as our modeling engine, and Oracle 10g
as our primary RDBMS. MS SQLserver, MySQL, PHP, .NET, and SAS and R are also available and wid
ely used. Web
content is served via Apache and Tomcat, as is our locally
written GUI to the RDBMS. We are fully integrated over a 1
gigabit Ethernet LAN with full WiFi capability. For projects with computational demands beyond our capabilities we have
ll access to the Texas Advanced Computing Center (TACC) at UT Austin. TACC is one of the largest and most
advanced HPC centers in the world.

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We routinely perform graphical 2 and 3D visualization of genomics and proteomics data, a wide range of univariate
multivariate statistical analyses, and various cluster and data reduction analyses. We have a number of site licenses for
important software suites such as MatLab (including the Bioinformatics Toolbox, SimuLink, and SimBiology), the
Affymetrix Microar
ray Suite 5.0/GCOS, and a number of concurrent licenses for the Ingenuity Pathways Analysis suite
with full access to their knowledge bases. We have licenses for several major proteomics packages including Nonlinear’s
Progenesis and Progenera, Genologic’s

Proteomics and Genomics LIMS, Ciphergen’s ProteinChip 3.2 with Biomarker
Wizard, Ciphergen Express 3.0 (CE) and Biomarker Patterns 4.0 for SELDI MS proteomics and biomarker discovery. We
have phylogeny programs including PAUP and BEAST. For classificati
on and biomarker discovery projects we have the
CART (Classification and Regression Trees), RandomForests and MARS (Multivariate Adaptive Regression Splines)
packages from Salford Systems as well as Support Vector Machines and other machine learning softwa
re. For NGS
bioinformatics analysis and data mining we have an increasingly large base of installed software on our Dell Linux cluster
including the state of the art CLC Genomics Server, CLC Genomics Workbench, and the CLC Bioinformatics database
from CLCb

Biomedical Imaging Network

is a single institutionally
based cost recovery center that will provide the expertise and
resources needed to effectively integrate and utilize imaging sciences and technology with basic and tran
research within UTMB and Texas.

Currently, the Biomedical Imaging Network will provide resources and services for two major areas: 1) optical microscopy
and cellular imaging and 2) small animal imaging. The optical Microscopy core is equipped w
ith a wide range of
instruments that can be used for advanced cellular imaging; live cell imaging and intravital optical microscopy. The
Animal Imaging core is equipped with Inveon Micro
SPECT imaging system; Visualsonics whole
body high

ultrasound imaging for small animals; high resolution optical endoscopy using in vivo fluorescence confocal
imaging and optical coherence tomography for small and large animal studies; and IVIS 200 bioluminescent and
fluorescence whole
body imaging workst
ation. The Biomedical Imaging Network is directed by Dr. Massoud Motamedi.
Dr. Gracie Vargas and Dr. Rinat Esenaliev are available to provide technical expertise for advanced optical microcopy and
ultrasound imaging, respectively.

The Optical Microscop
y and Cellular Imaging Core

assists with high
resolution imaging and analysis of specimens,
including those exposed to infectious agents and/or treated with toxic substances. Agents with infectivity can be accepted
up to biosafety level 2, and fixed specim
ens lacking infectivity will be accepted for any infectious agent authorized on the
UTMB campus. The OIC is equipped with a Zeiss LSM 510 UV META laser scanning confocal microscope, a large format
fluorescence microscope (Zeiss Axiophot 2) with attached hi
resolution digital camera and a SLM 4800S life
spectrofluorometer. The confocal microscope includes: an Axiovert 200M microscope; dry, water
, and oil
immersion high
resolution objectives for fluorescence and Normarski differential interference con
trast observation; Ar, dual HeNe, and UV
lasers; fluorescence filter sets for DAPI, FITC, TRITC, INDO
1, and SNARF; a scanning module with visible and UV
acousto optical tunable filters, two independent fluorescence channels (2 PMTs), a 32
PMT array; and a

stage incubator.
This instrument is equipped for high
resolution detection of cellular and infectious agent
specific proteins and in particular
for monitoring changes in their volume distribution relative to changes in physiologic parameters. A high

(Dell 530 work station with dual 1.8 GHz xeon processors) work station with two monitors and software for physiology,
scan and 3
D imaging supports the image analysis activities within the OIC.

Optical Imaging Laboratory


igital imaging sys
tems that include confocal and wide
field optical microscopes:
Zeiss LSM 510 confocal microscope and spectral imager,
capable of multicolor three dimensional fluorescence
imaging for colocalization; fluorescence resonance energy transfer (F
RET), and multispectral analysis

of single cells or
thick specimens.
An advanced semiautomatic wide field imaging system is
capable of quantitative time lapse studies
combining multiple microscopy modes simultaneously. The u
pright wide field imaging system

is equipped with color and
monochrome digital CCD cameras, ideal for studies of fixed samples using color bright field, phase contrast, DIC and
multicolor fluorescence.
Image processing and analysis:
The facility has 4 PC stations equipped with Metamorph

Image J imaging, and other graphical software.
Fully assisted or independent operation:
depending on user needs, we
provide complete support for microscopy sessions as well as image processing and analysis; however we also allow more
experienced users

to perform their own experiments

and observations. The lab is a
vailable 24 hours a day, 7 days a
week. Online services are available via
our website under “
support and customization.” Training, supervision and
We offer training on microscopy a
nd digital imaging.
Other equipment and services are available u
pon request.
For example, we can install perfusion systems, temperature recording and control, micromanipulators and patch clamp
recording setups.

The Biomolecular Resource Facility (BRF)
A. Kurosky, Director, is a UTMB
designated core facility providing research
support targeted to the analysis of biomolecules, especially proteins and peptides. Originated in 1975, the facility has
grown consid
erably to meet the analytical needs of UTMB investigators. The BRF is composed of 7 core laboratories
whose services are briefly outlined below. A more complete description of BRF services can be found at

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The BRF overarching philosophy is to provide, at reasonable cost, biomedical researchers with a
relevant menu of analytical services that will enhance their funded research projects and the quality of their publications.
Importantly, the BRF puts con
siderable effort into obtaining major state
art instrumentation too expensive for
individual investigators. Researchers are encouraged to interact closely with BRF personnel to obtain maximum benefit of
services. User fees are minimal, compared with

other institutions, largely due to support from several UTMB centers, e.g.
NHLBI Proteomics Center, NIAID Clinical Proteomics Center, NIEHS Center, and the Sealy Center for Environmental
Health and Medicine. The BRF occupies 6,461 square feet, distributed

among 12 laboratories and 7 offices in the Basic
Science Building along with 588 square feet of three office suites on the 1


The current scientific staff includes 18
individuals (1 M.D./Ph.D., 8 PhD, 3 MS, 6 BS).

Biosynthesis & Biomarker Core

Services include

a) biosynthesis of recombinant proteins in bacteria or yeast
cultures; b) protein purification and characterization; and c) polyclonal antibody production.

Mass Spectrometry Core


Services are a) MALDI

TOF/TOF MS/MS analysis; b) ele
ctrospray ionization MS/MS
analysis, including liquid chromatography (LC/MS/MS); c) protein ID analysis; d) post
translational modification
identifications, and e) stable isotope methods (e.g. iTRAQ and


Peptide Synthesis Core


Offers a) FMOC auto
mated solid phase synthesis; b) peptide purification, and c) peptide
mass analysis.


Protein Chemistry Core


Provides a) DNA sequencing; b) protein sequencing; c) amino acid analysis, and d)

carbohydrate analysis.


Proteomics Bioinformatics Core

ides a) nonlinear 2D gel analysis; b) Genologics LIMS for data/sample
management and access, and c) database searching.

Separations Technology Core

Provides a) HPLC analysis; b) 1D and 2D gel electrophoreses; c) fluorescence gel
staining and imaging, a
nd d) robotic gel spot picking and processing for MS analysis.

The Office of Biostatistics (OBIOS)

provides statistical support services to all faculty at UTMB, including designing
studies that minimize the effects of measurement and experimental error, identifying appropriate methods for data
analysis based on st
atistically sound designs, producing power calculations, and determining sample. OBIOS uses the
computer packages such as NCSS

and SAS, statistical applications with extensive data management capabilities.

Adaptation and development in
clude quanti
ve research

that will produce im
proved methods and procedures to analyze
and interpret biomed
cal data.

This usually occurs during a long
term collabo
ration between a UTMB research
er and a
member of the Office of Biostatistics.

Special services are
available to assist with the conceptualization, formulation, design, and use of data gathering tools.

The Environmental Exposure Facility

provides state
art capabilities for conducting exposures of experimental

in vitro

models to gas phase environmental toxicants.

A service core of the UTMB Sealy Center for
Environmental Health and Medicine, it supports all UTMB investigators and students on a fee
service basis.

addition, the facility provides a fou
ndation for inter
institution collaborative investigations.

The facility is housed in an 800
square foot, three
room suite. In addition to a room for exposure chambers, an animal housing room and a room for
conducting in
vitro exposure of cells in culture

make up the facility. Four 0.8 M

stainless steel Hinnars
type exposure
chambers are available for exposure of small laboratory animals to low concentrations of gas phase chemicals. The
facility is equipped for studies using organic chemical vapors, ozone

or nitrogen dioxide. The chambers receive filtered,
conditioned, building air at a rate of 30 chamber changes per hour. Regulation of gas concentration is accomplished
using mass flow controllers. Organic vapor concentrations are monitored by gas chro
matography. Concentrations of
ozone or nitrogen dioxide are determined by dedicated gas monitors. The suite is maintained under negative pressure
relative to the surrounding building spaces to insure that any exchange of air is from the building into the f
acility, which
also has safety interlocks that shut down gas and air flow in the event of a power interruption or if a smoke detector is
triggered. The in vitro exposure facility uses similar equipment to deliver gases to small glass chambers placed on til
tables in cell culture incubators.

The Flow Cytometry Core Facility

in the Department of Microbiology and Immunology
is located in the Medical
Research Building room 3.159. It is directed by Dr. Jiaren Sun, Associate Professo
r in the Department. Its daily operation
is managed by Mark Griffin and assisted by Dr. Lifei Hou. Mark is an expert in flow cytometry and cell sorting with many
years of experience. Lifei is a talented immunologist, highly competent in experimental design
, instrumentation, data
analysis, and assay development.

The facility is equipped with one high
speed cell sorter as well as three analyzers suitable for various tasks and levels of
user friendliness. The BD


are advanced analyzers

that can detect 18 and 6 colors,
respectively. The

offers automatic sample loading with a 96 well plate reader that allows for walk
away use. The

has a carousel loader with similar convenience features. The third analyzer,
Accuri C6
, is cap
able of detecting 4

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colors, and is particularly user friendly. All analyzers are available to independent users with 24/7 access. Users can
acquire necessary hands
on skills either through formal or informal training or attending one of our training sessio
ns (to be
announced later). The BD

is available to users as an operator
assisted sorter, which can separate cells into
tubes, plates, or slides at high speed. Direct cloning of sorted cells into tissue culture plates is also possible under asep

conditions. In addition to its cell sorting functionality, it is capable of 9
color analysis in its present configuration.

The flow lab can also provide assistance with protocol development and implementation, as well as data analysis and
presentation. Th
e data from the
Aria, Fortessa


are available campus
wide over the university's intranet for 1
month, such that investigators may analyze and store data in their own labs. For scheduling work, users may email

or call 409
2011. The operator’s assistance is available 9
5 daily. A schedule with
instrument availability is kept on

r user planning and service reservation. The
Accuri C6

is for users requiring no assistance available on a first
come first
served basis.

The Institute for Translational Sciences (ITS) and the Clinical and Translational Scienc
e Award (CTSA)

The ITS and its collaborators work to enhance the scope of translational research at UTMB by creating an environment for
rapid translation of basic research into diagnosis, treatment and prevention of disease and to facilitate the full spect
rum of
translational research, from T1 (bench to bedside) through T4 (community research, outcomes, adoption of best

The ITS is the academic home of UTMB’s CTSA, designed to facilitate translational research as a rigorous discipline,
translational research training programs, conduct early phase translational research and extend translation to
later phases, and interface with a national consortium of CTSA institutions. We have organized our CTSA as 12 “Key
Resources”, aggregations of e
stablished university core laboratories and existing and new intellectual resources.

Because of their importance to translational research, several of UTMB’s core laboratories and centers of excellence are
administered in partnership with the ITS and CTSA.

These include the: Sealy Center for Molecular Medicine, General
Clinical Research Center, Bioinformatics Program, Recombinant DNA Laboratory, and Biomolecular Resource Facility.

Coordinating Core:

Assists in addressing logistical, administrative and comm
unications barriers to conducting
multidisciplinary, team
based research.

Serves as a single point of contact for investigators and trainees interested in translational research

Facilitates communication among multidisciplinary translational teams (MTTs)
and CTSA Key Resources

Maintains a pool of study coordinators

Communicates the availability, significance and results of trials

Novel Methodologies:

Helps inv
estigators develop novel research approaches and enables use of tools and technologies
for solvi
ng human health problems.

Facilitates integration of advanced imaging techniques for diagnosing disease and for monitoring the safety and
efficacy of new therapeutic interventions

Builds and deploys infrastructure for image acquisition, image analysis, vis
ualization & data management
software tools

Leads the “Catalysts for Innovation Leadership Council”.

Awards pilot funding for new methodologies that accelerate translational research and product development

Pilot and Collaborative Studies:

Supports pilot
projects, projects involving novel methodologies and collaborative studies
that will have a significant impact on clinical and translational research.

Provides financial support for multidisciplinary teams, individual investigators and trainees engaged in

translational research

Supports the creation of new research teams by funding projects to collect preliminary data leading to
independent funding at the equivalent of an R01 level or higher

Translational Technologies and Resources:

Facilitates acquisit
ion, understanding and the use of technologies that enable
effective translational research.

Provides expertise in sample collection, handling and processing to ensure high quality clinical data outcomes

Ensures that data is accurately managed and transla

Facilitates use of standard protocols

Provides access to biorepository

Provides access to the Biomolecular Resource Facility

Assists with technology acquisition, development, data analysis, and quality assurance

Biomedical Informatics:

Provides a mul
investigator, multi
institutional environment for sharing biomedical data and
establishing a deep data
mining collaborative infrastructure.

Provides tools and personnel to significantly improve your ability to capture and analyze data efficiently,
ctively and securely

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Ensures innovation in data collection and analysis

Provides expertise in deep data mining which enables improved IP discovery

Provides clinical informatics expertise to facilitate significant new innovations

Biostatistics, Epidemiolo
gy and Research Design:
Works with investigators to enhance the efficiency and quality of
research by providing integrated statistical support. By proactively engaging in the research development process, we
help clinical and translational investigators in

research design and analysis.

Provides expertise in biostatistics, epidemiology and research design

Facilitates web
based collection of data to capture costs and outcomes to assure estimation of efficiency (T3)

Provides a link to the Medical Bioinformatic
s system and EMR

Improves the design and implementation of evaluation

Provides integrated study statistical analysis and sample size planning

Ensures variance minimization

Increases understanding of the impact of common cause variation.

Regulatory Knowledg
e and Support.
Expedites implementation of your research by helping you navigate the complex
regulatory environment. Our services and tools are comprehensive and researcher
focused and are designed to remove
institutional impediments to research.

ies and disseminates best practices and system improvements for regulatory management and compliance

Assures compliance, so that data can be used for publications, FDA applications, etc…

Protects the integrity of your research

Develops study
specific SOPs

Completes and manages IND applications

Facilitates translation of informed consent documents into Spanish

Registers studies at:

Complete IRB documents

Assures adherence to Data Safety Monitoring Plans / Boards

Clinical Research Ce

Provides modern equipment and experienced personnel to support your inpatient and
outpatient studies.

Offers training in clinical research

Assists with subject recruitment

Provides access to multiple clinical research sites

Hosts outpatient and in
patient stays

Schedules patients

Provides nursing and dietary support

Collects blood, saliva and tissue samples

Provides access to a metabolic kitchen and metabolic cart

Provides access to underwater weighing, exercise equipment, and DEXA imaging

ity Engagement and Research:

dvances population
based translation of science by establishing and
maintaining infrastructure to represent community interests among prospective researcher partners. We can also support
your linkages to community partners, a
nd coordinate use of relevant community assets.

Provides education in the principles of Community
Based Participatory Research (CBPR)

Maintains and coordinates a multidisciplinary Practice
Based Research Network

Facilitates relationships with community par
tners interested in participating in research opportunities

Familiarizes research teams with the work of other researchers or clinicians working with community groups

Identifies opportunities for collaboration

Disseminates the results of research to public

health and policy
making entities as well as community groups

Assists with preparation of NIH proposals that require community engagement components

Research Education, Training and Career Development:
Enhances trainee quality and productivity by develop
ing and
integrating activities and programs at many levels. Our goal is to support biomedical scientists with the skills to become
productive members of MTTs.

Identifies and recruits qualified students and trainees at the doctoral and postdoctoral level (
MD and PhD) and
provides them with research experiences and curricula for developing skills in translational and clinical research

Implements the ITS Translational Scholars program, to help faculty establish careers in interdisciplinary
translational and c
linical research

Ethics and Support:

Provides expertise and education in research ethics and research integrity and assists in developing
ethically sound protocols and practices that enhance subject safety and rights.

Integrates knowledge of best practic
es and engage teams in discussion of ethical issues

Increases understanding of emerging issues (e.g., incidental findings, specimen storage, re
identification of data,

Ensures that your research respects the autonomy of study participants

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Assesses t
he risks and benefits of your research

Helps researchers explore the social value of their research

Helps researchers avoid the therapeutic misconception in the informed consent process

Enhances privacy and confidentiality in data registries

Assists with

protocol design, consent, and recruitment

Facilitates IRB approval process

Assists with problem
solving and conflict

Deepens partnerships between ethics and science

Maintains focus on beneficial health outcomes

Provides advice on research integ

Tracking and Evaluation:

Assists researchers by providing training in program evaluation and team building

Develops evaluation processes

Enhances the tracking of team progress

Clarifies challenges and growth opportunities related to team development

The Research Histopathology Core (RHC)

occupies 2800 square feet of laboratory space in the Basic Science Building,
and provides analytical morphology related services to the research teams and educational programs at UTMB. Th
facility is equipped with the latest available instrumentation and operated by highly skilled personnel, who can assist with
tissue preparation, Immunohistochemistry including assay optimization, image analysis, digital photomicrography,
anatomical consu
ltation, and

Hybridization. RHC provides an expanded service including coordinated
histopathology support services or develop special protocols to fit your research needs for investigators engaged in
research in emerging diseases. We look forward to

providing these services to fit your research needs and yield high
quality results.

Research Technology Support (RTS)
epairs and maintains laboratory equipment,
such as centrifuges, incubators,
physiological monitors, scales
, microscopes, etc. RTS also provides preventive maintenance and electrical safety
inspections. Through the c
omplete machine shop, with CNC capabilities, RTS also works with investigators to
structures in Lexan, Plexiglas, standard and exotic met
als for specialized research needs.

The Rodent
In Vivo

Assessment (RIVA) Core

was established by the Center for Addiction Research to facilitate the
advancement of interdisciplinary and translation research on and off

We provide rodent models for analyses of
new transgenic mice

and rats, as well as a wide variety of

research tools ideal for drug discovery and evaluation of
chemical entities for therapeutic potential.

Our facilities include 12 behavioral procedure rooms equipped with state
art computer driven data collect
apparati, three animal holding facilities and a surgical suite. Multiple assays are available, and can be adapted to many
questions. Services range from consultation and training to study design, execution, data analysis and interpretation. For
hers interested in a more hands
on approach, we will provide appropriate orientation and training, leaving the data
collection to individual lab personnel with minimal supervision. For those preferring a less
involved approach, studies may
be arranged to b
e performed by RIVA personnel. We are a fee
service facility and will gladly provide a quote for any
level of service.

Sealy Center for Cancer Cell Biology


The Transgenic Mouse Core Facility (TMCF)
was established as a university core in 1996. It is the sole core facility
that specializes in the generation, maintenance and preservati
on of genetically engineered mice in UTMB. To date, the
TMCF generated over 80 strains of genetically engineered mice. The TMCF is supported by user cost
sharing, grants
funding individual investigators, and several departments including the Comprehensiv
e Cancer Center, Department of
Neurology, Department of Biochemistry and Molecular Biology, Department of Neuroscience and Cell Biology, the Mitchell
Center for Alzheimer’s disease, and Research Services.

The mission of the TMCF is to provide comprehensi
ve and flexible support to investigators who engage in genetically
engineered mouse model research, and to facilitate the growth of the mouse research and translational research in the

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The TMCF is available for use by all UTMB scientists. We can pr
ovide guidance and technical support at all stages of the
development of genetically engineered mouse models. We can serve as a mentor of your student, your collaborator, or
your hand.

Mouse Laboratory

was established in 1983 to serve UTMB investigators interested in establishing human cancer
xenograft lines and studying the effects of various agents on tumor growth in an
in vivo


Because tumors generally
maintain their h
istology and human karyotype and because human drug sensitivity or resistance are stable when tumors
are transplanted to the nude mouse, the laboratory both enhances and promotes research related to human cancerous

In addition, it enables investig
ators to apply for grants and contracts related to human cancer research, many of
which specifically require experimentation on nude mice.

The establishment of uncommon cancers can be facilitated by
transplantation into the nude mouse.

Finally, various a
gents and drug delivery systems may be tested using this
in vivo


Specific objectives are to: assess specific therapies and drug delivery systems on the growth of different human cancers
in nude mice; provide source materials for human tumors; use
the T
deficient nude mouse for immunological studies;
provide an immunocompromised
in vivo

model; assess developmental growth and gene expression of normal tissues
from other species; and facilitate the establishment of uncommon tumors resected from p

Many colorectal, gastric and pancreatic cancer xenograft lines have been established and are available for analysis and

In addition, novel human endocrine cancers have been established that represent, in some instances,
kind tumor models.

The majority of tumors are transplanted to the subcutaneous tissue of the back or flank.

This allows for ease of analysis and measurement.

Further, tumor models are available that involve implantation into the
abdomen and mimic human
metastatic cancers with metastasis to the liver or other organs.

Injection of test agents can
be achieved by the oral, subcutaneous, intraperitoneal or intravenous routes.

Novel methods of drug delivery may be
relatively easily assessed using nude mice.

Time PCR Core Facility

offers a full complement of real
time PCR (polymerase chain reaction) services including
primer and probe design and order, preparation of real
time PCR reactions, and data analyses for gene expression (DNA
and RNA samples)
, as well as allelic discrimination. The PCR core facility has the following major equipment available:
Applied Biosystems (ABI) PRISM 7000 sequence detector with a Dell Pentium III laptop computer; a Dell Pentium IV
desktop computer installed with the ABI

PRISM 7000 SDS (sequence detection system), PrimerExpress and file builder

The UTMB Tumor Bank

is housed in the Sealy Center for Cancer Cell Biology as part of our efforts to facilitate cancer
research at UTMB. Collection of tumor tissues is a collaboration of the SCCB and the Division of Surgical Pathology. This
bank contai
ns >2,000 different specimens of various tumor
types, The Human Tumor Bank Core provides tissues for
related research approved by the Institutional Review Board (IRB) and the Biological Safety Committee for using
biohazard materials. In addition, th
is service promotes material
sharing and provides assistance on cancer tissue

The Biostatistics Shared Resource (BSR) Facility

operates under the auspices of the UTMB Cancer Center. The BSR
serves as a centralized resource for comprehensive and high
quality statistical support to all aspects of cancer research,
including basic
science, translational research and clinical studies.

The Cancer Center’s BSR Facility provides the following services to cancer researchers:

Study design and determination of sample size to optimize resource utilization

Planning of
in vitro
in vivo

ies of animal cancer models and translational studies

Design and oversight of clinical trials

Monitoring of safety/toxicity and efficacy parameters in clinical trials

Development of database and information management systems

Generation of interim stat
istical reports

Application of innovative statistical methodology in cancer research

Analysis and interpretation of final statistical data

Training and teaching through cancer specific programs at UTMB

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The Oncology Clinica
l Trials Office (OCTO)

serves as a full service, multidisciplinary resource for University
investigators and clinical study sponsors who are committed to performing the highest quality clinical studies. OCTO is a
resource that provides the necessary tools
and expertise for conducting innovative and safe clinical trial research in
compliance with required federal guidelines. The OCTO mission is to:

Facilitate patient access to oncology clinical trials by providing up to date information about ongoing clinic
research at UTMB

Support investigators in every aspect of oncology clinical trials research, including protocol development and
review, contract execution, financial accounting, budget negotiations, compliance reporting and trial

Serve as li
aison between PI’s and clinical sponsors

Sealy Center for Molecular Medicine Cores


Molecular Genomics (MG) Core Laboratory

provides services, technical support and advice to UTMB
investigators in the area of genomic analysis. The MG Core provides gene expression analysis through the use

Affymetrix gene chips and real time arrays, SNP analysis (both association
based studies and discrimination
assays), real time quantitative RT
PCR, miRNA analysis, DNA sequence analysis and high resolution melt


Recombinant DNA Laborat

provides UTMB investigators with a variety of essential services and
technical support in the area of recombinant DNA techniques.

Services include the production of plasmid DNA,
transformation and screening of recombinant plasmids, bacterial expressio
n, site directed mutagenesis,
construction of transgenic and null gene plasmids and various PCR based technologies.

The Sealy Center for Structural Biology & Molecular Biophysics (SCSB) Research Laboratories


h Laboratories

aid scientists interested in elucidating the basic relationships between
macromolecular sequence, structure and function, with a goal of leveraging this information into the understanding and
treatment of disease. The basic research within S
CSB combines thermodynamic, kinetic and structural data about
biomolecules and their complexes to understand important biomedical processes. The structural and dynamical basis for
molecular recognition, signal transduction, protein folding and allosteric r
egulation is studied by X
ray crystallographic
> , nuclear magnetic resonance, <

electron microscopic <

techniques, advanced computational tools,

and solution thermodynamics <
> .

The SCSB Research Laboratories are Research Cores requiring serious time investment by resear
chers. Accordingly,
each Laboratory offers training in use of instrumentation, software and
design, along with workshops on
specific topics.

Graduate students and post docs may take the instruction provided by
in conjunction with
c courses in NMR
, X
ray Crystallography, CryoEM, or Molecular Biophysics

by SCSB faculty
through the
GSBS. Unless researchers have the appropriate expertise in their own laboratories most projects will require the expertise
provided by collaboratin
g with the Center, either
aculty member or one of the


initiate a research project in the Center please contact
a SCSB faculty member
, the
specific Laboratory

or the
Center Director, Wayne Bolen.

The Computational Biology Research Laboratory
in SCSB provides modern computational resources to researchers
and to students who are in need of large
scale computing power. The power house of the

is a 30 processor
Linux cluster, which provides parallel
processing capabilities with a total of 84 billions floating point operations per second,
42 GB of memory and 1.6 TB of storage space. The cluster also provides centralized file
server capabilities as well as a
tape l
ibrary with 400 GB of storage space to archive and secure data. Peripheral devices include a scanner, B&W and
color printers, plus tape drives. All computing resources are connected to the UTMB network, and thus other workstations
within the campus can eas
ily access the resources of the
. An account request form is available online

(Manager, David P

The Cryo Electron Microscopy Research Laboratory
, on the first floor of the Medical Research Building, has laboratory

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space featuring a BSL
3 room for viral and pathogen work. The Laboratory has three JEOL cryo
electron microscopes,
used for imaging

of macromolecular complexes, cell organelles and other biological systems. The high
JEM2200FS is located in the BSL
3 facility and permits structural imaging of pathogens. Unique to the core is the W. M.
Keck Center for virus imaging with BSL

containment, the only cryo
EM facility in the U.S. designed for high
structural studies of wild type infectious agents. The JEM2100 is available for imaging of non
pathogenic targets. A lower
resolution JEM1400 microscope is available for preli
minary screening.

Image data are stored in a local database that is
archived remotely. Microscope scheduling, for qualified users, is available online.

The NMR Spectroscopy Research Laboratory.
ocated in its own two
story bu
ilding (Bldg. 73) at the Universit
y of
Texas Medical Branch the SCSB NMR Research Laboratory
art high field Varian VNMR system
800MHz (with a HCN Cold Probe),
VNMR system 750MHz



Inova 600 MHz NMR spectrometer
with 31P NMR
capability. Both

the 800 and 750 MHz instruments are capable of HCN triple
resonance experiments with 2H decoupling.
Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool in studies involving structural biology, proteomics,
metabolomics, metabon
omics, nutrition, toxicology, analysis of biological fluids, functional genomics, structure/activity
relationship, drug discovery and development, macromolecule structure/function analysis and small molecule structural

The NMR techniques used i
n such studies include: relaxation measurements, chemical shift mapping,
hydrogen exchange, conformational exchange, transfer NOE, Saturation Transfer Difference(STD), dynamics and 3D
solution structure determination. Spectrometer scheduling, for quali

users,is available online.

The Solutions Biophysics Research Laboratory
, located on the fifth floor of MRB,

is equipped with a Beckman
Analytical Ultracentrifuge XL
A to monitor the hydrodynamic properties of biomacromolecule
s and protein
interactions, a GE Health Sciences' Biacore T100 SPR high
throughput system for measuring the kinetics of protein
protein and protein
ligand interactions, a Johnson and Johnson ThermoFluor, high
throughput ligand screening system for
the discovery of protein
ligand interactions, a MALDI Mass Spectrometer for use in monitoring the quality of
biomacromolecules samples, a MicroCal Isothermal Titration Microcalorimeter, and various spectroscopic instruments.

High throughput analyses are f
acilitated by an epMotion 5075 Solution Robot for sample preparation. Solution biophysics
and thermodynamics techniques are used both as primary research tools and to guide structural studies.

ray Crystallography Laboratory

in the SCSB provides complete facilities for crystallization studies of macromolecules.


houses a Rigaku Crystalmation® robotics system, consisting of an Alchemist
II for preparation of crystal
screens, a PHOENIX, for fast reliable and effic
ient screening of crystallization conditions, and a DT Minstrel/Gallery 160
for automated imaging of crystallization trays. Two x
ray area detector systems are available for the collection of X
diffraction data, each with an ultra
focus high
iancy x
ray generator and focusing multilayer optics. These are
suitable for protein crystals from 50 microns in size with cell dimensions up to 400 Å and are equipped cryogenic sample
cooling systems.

The Crystallographic Computation Lab provides high
rformance Linux stereographics workstations for
crystallographic structure solution, refinement, and analysis or CryoEM reconstructions.

Diffractometer and robotics
scheduling, for qualified users, is available online.

Sealy Center for Environmental Health and Medicine (SCEHM)

The Synthetic Organic Chemistry Core

provides an on
site, cost
efficient, full
service, chemical synthesis facility to
synthesize defined DNA lesions, bioconjugates an
d small organic molecules that are unavailable or commercially

The SOCC is part of the SCEHM and the NIEHS CET at UTMB and has been in operation since March 1998.

Since then it has synthesized more than 800 different organic synthesis products

for a number of researchers and
research cores on and off campus.

SOCC synthesis projects include modified nucleosides and oligonucleotides, metal
chelators, steroid analogs, modified peptides, enzyme substrates and inhibitors, bioconjugates, as well as
hard to find
organic analytical standards and reagents. The lab is a shared chemical synthesis facility also open to researchers who
need assistance with chemical syntheses, wish to use the lab’s resources, or need technical supervision and/or training of
technicians on research synthesis projects.

The 1200 sq ft lab, located in room 2.130 of Ewing Hall, houses equipment
for multi
step organic chemical syntheses from mg to 100g scales, modified DNA syntheses, HPLC analyses and
purification, photochemical r
eactions, flash chromatography, rotary evaporation, distillations, sublimations, titrations,
temperature controlled and high pressure reactions.

The Tissue Culture Core Facility
is a division of the Department of Microbiology
and Immunology. The core supports
UTMB investigators in a time

and cost
efficient manner. The facility provides products for tissue culture, molecular
biology, and immunology research from multiple vendors. Other services include heat in
activation of ser
um, mycoplasma
detection and eradication, tissue culturing, and cryogenic preservation and repository of cells.

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The Galveston National Laboratory (GNL)

The Galveston National Laboratory (GNL) is an academic research center at the University of Texas Medical Branch in
Galveston, Texas.

Utilizing the unique resources of its BSL2, 3 and 4 labora
tories, the GNL’s renowned scientists work
collaboratively, both locally and internationally, tackling some of the world’s most pressing health concerns.

strengths in aerobiology, assay development, experimental pathology, imaging, immunology, insect
ary services and
preclinical studies, the GNL advances local discoveries on the diagnostic tests, treatments and vaccines for the infectious
diseases impacting global health like tuberculosis, SARS virus, West Nile virus, Ebola, Marburg, Plague, encephalit
influenza and a host of others. The GNL was funded in part by the National Institutes of Health (NIH)/National Institute of

Allergy and Infectious Disease (NIAID) and serves as a national resource in support of NIAID’s biodefense and emerging
ous diseases research agenda standing ready to assist local, state and national public health efforts in the event of
a public health emergency.

The GNL and adjacent facilities offer more than 14,000 square feet of BSL4 space, over 29,000 square feet of BSL3
facilities and nearly 52,000 square feet of BSL2 facilities.

These facilities include typical lab spaces as well as animal
biosafety lab
space (ABSL), insectaries and aerobiology facilities.

UTMB is a registered select agent facility for CDC,
USDA and overlap agents.

The GNL’s mission is fulfilled through a system of specialized services available to researchers in biodefense and
g infectious diseases via the following Service Divisions:


The Aerobiology Services Division offers aerosolization capabilities to researchers examining BSL3
and BSL4 level bacteria and viruses via inhalational exposure. This division uses sp
ecialized equipment to
expose models to various pathogens including select agents such as anthrax, plague and Venezuelan equine
encephalitis, to allow examination of disease mechanisms and to evaluate the efficacy of potential

Assay De

The Assay Development Services Division provides assay development and drug
screening for identification of new therapeutics for researchers who study select or emerging disease agents.
Division personnel generate assays that are standardized,
reproducible and useful in medium

to high

screening. While the emphasis is on developing low
containment BSL2 assays, the division also designs and
tests assays suitable for BSL3 and BSL4 containment.

Experimental Pathology:

The Experimenta
l Pathology (EP) Services Division features support for tissue
studies and tissue culture needs of researchers.

EP offers routine histopathological services (tissue processing,
microtomy, cryotomy, cytospin and smear preparation, routine and special

histochemical staining),
immunohistochemistry, immunofluorescence, and
in situ
hybridization analyses.

EP Division staff provide expert
assistance in staining and tissue preparation for advanced microscopy studies (confocal or two photon
microscopy) in c
onjunction with the Imaging Services Division.

Facilities for light microscopy, digital
photomicrography, image analysis, fluorescence microscopy, and laser capture microdissection are available.

The unit’s Tissue Culture Core facility also exists as a s
ource of scientific supplies, such as media, sera and
enzymes, and serves as a safety
compliant shipping and receiving unit. In addition, tissue
based diagnostic and
etiological assessments from EP experts are available to public health entities in the eve
nt of disease outbreaks.


The state
art Imaging Services Division provides advanced optical analysis for
in vivo
animal studies and tissue specimens at BSL2,
3 and
4 biocontainment levels.

The unit offers combined
confocal and m
ultiphoton microscopy imaging systems at the BSL2 and BSL3 levels for molecular imaging and
microscopy, an IVIS 200 system for
in vivo
small animal molecular optical imaging, a micro PET/CT whole
imaging system, digital X
ray, and ultrasound equipme
nt. Also available are a full complement of gel
capabilities, as well as traditional X
ray services.


Immunology Support Services Division capabilities include the generation and maintenance of
knockout and transgenic mouse models fo
r immunological examinations of select and emerging disease agents.
In addition, the Division develops, from these models, primary cell lines for use in
in vitro
experiments and
provides experimental surgery services and production of human monoclonal anti

Insectaries Services:

The Insectaries Services Division (IS) provides expert consultation and state
equipment for research into arthropod
borne biothreat agents and emerging infectious diseases. IS facilitates
research by biodefense

investigators who require high and maximum containment for studies with arthropod
vectors by either providing expertise and/or training for the researchers’ personnel. In addition to mosquito and
tick rearing in Arthropod Containment Level two (ACL2) faci
lities, specialized resources and equipment located
within ACL3 facilities include, membrane feeding systems, intrathoracic inoculation apparatus, chill tables,
environmental chambers, trituration apparatus, containment glove
boxes, microscopy and imaging

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Preclinical Studies:

The Preclinical Studies Services Division supports testing of vaccines, diagnostics and
therapeutics against challenges from biodefense and/or emerging disease agents. Division expertise includes
surgical implantation

of telemetric devices, as well as conducting laparoscopic, endoscopic and ultrasound
tissue biopsies. The division’s aim is to provide real
time data with minimally invasive techniques. The division
accesses, as appropriate, the GNL’s other service
s, such as the animal support unit, which has facilities for both
small animals and non
human primates, and the services of the Aerobiology Services Division.

The services and specialized equipment these Divisions provide, amon
g others, include:

aerosolization studies of BSL
3 and
4 level pathogens in small animals and non
human primates;

animal model development;

pathogen studies;

vaccine, diagnostics and therapeutics testing;

screening methodology development through

in vitro


technical assistance in histological and tissue processing methods;

diagnostic expertise in the event of disease outbreaks or bioterrorism,

protocol development against arthropod vectors;

imaging expertise in the analysis of human an
d animal tissues and
in vivo

animal imaging specialized
imaging equipment, including combined confocal and multiphoton microscopy imaging systems at the BSL
2 and
3 levels;

IVIS 200 system for
in vivo

small animal molecular optical imaging;

roPET/CT whole animal imaging system;

digital X
ray and ultrasound equipment;

a full complement of gel imaging capabilities as well as a traditional X
ray film processing

a full
service immunology core capable of developing knockout and transgenic mouse
models for immunological

GLP study oversight

service animal care procedural and maintenance facilities

a Tecan robotic liquid handling system and other robotics instruments for assay development

a full complement of thermal cyclers, includ
ing RT
PCR machines, a BSL4 level flow cytometer and a BSL3 level
fluorescence activated cell sorter.

There is also a fully equipped histopathology suite, ultra
, super
, and low
speed centrifuges; and glassware washing and sterilization equipment and aer
osol challenge facilities allowing
the safe study of inhaled viral and bacterial pathogens via Madison chamber units and equipment capable of
facilitating nose
only exposure.

The University has an existing safety program that is in accordance with appropr
iate federal, state, and local regulations
for research performed safely under BSL2, BSL3 and BSL4 laboratory conditions.
UTMB is also home to the
National Biocontainment Training Center

which prepares infectious disease scientists to work safely in hi
gh and
containment environments.

Through extensive one
one, customized didactic training, practical
demonstration and mentored experience in the lab environment, the NBTC’s Laboratory Biosafety Training Program
provides laboratory and support s
taff training in BSL2 to 4 laboratory biosafety.

The NBTC also offers parallel fellowship
tracks for scientists and biocontainment engineers, affording individuals the unique opportunity to gain valuable
knowledge and experience working in BSL3 and BSL4 l
aboratory environments.

The NIEHS Center Cell Biology Core

(CBC), an established National Institute for Environmental Health Sciences
(NIEHS) Center Service Core at UTMB, developed an infrastructure of core services for member
s of the NIEHS Center
Research Cores. This core is dedicated to the development and delivery of state
art methods and technologies in
cell biology to assist Center investigators in their mission. The wide range of diseases studied includes cancer, d
abuse, behavioral disorders, chronic inflammatory lung diseases, aging and age
associated diseases. Related research
ranges from studies of molecules (damaged DNA, DNA repair proteins, transcription factors, drug metabolizing enzymes)
and cells to thos
e of whole animals and human populations. The core supports investigators from the Departments of
Biochemistry and Molecular Biology, Pharmacology and Toxicology, Microbiology and Immunology, and the Sealy Center
for Molecular Sciences. In addition to stat
art equipment and methods, the key personnel of this core provide
scientific and technical expertise that greatly benefits investigators in terms of experimental design, execution and
interpretation. A particular strength of this core is to serve
as an integrating umbrella to foster collaborative arrangements
that cross departmental boundaries, and to facilitate productive interactions between basic and applied environmental

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Pepper Center Cores

Claude D. Pep
per Older Americans Independence Center


The focus of the UTMB Pepper OAIC through its first two funding cycles (2000
10) has been on age
related sarcopenia
and its contribution to loss of independence in older persons. The theme for the

cycle (2010
15) is ‘Translating
Biological Mechanisms of Muscle Growth and Loss to Improve Function and Recovery in Older Adults.’

The aims of the Pepper OAIC are to:


Provide core support to funded translational research by UTMB investigators on muscle f
unction and functional
recovery from i
llness in older adults


Stimulate the growth of additional interdisciplinary translational research projects to improve muscle function and
functional recovery from illness in older adults by funding pilot project resea
rch to generate preliminary data in
promising new areas of investigation and funding developmental projects to develop innovative technologies;


Train future leaders in research in the mechanisms, prevention and treatment of muscle dysfunction and disabili
in older adults;


4) Recruit established investigators with expertise relevant to muscle function and functional recovery in older
adults into interdisciplinary translational rese
arch related to the OAIC focus.


5) Foster collaborations between UTMB inves
tigators and investigators at other OAICs and other institutions on
studies of muscle function and functional recovery in older adults.

he Center focuses on translating basic discoveries on the mechanisms of muscle loss and re
growth with aging to
cal practice to promote functional recovery and prevent disability in older adults. Observational studies will provide
crucial information for selection of appropriate in
hospital, post
discharge, and long
term outcomes for inclusion in clinical
trials of
geriatric patient populations.

goal is to

identify predictors of physical function and recovery from illness in hospitalized geriatric
patients, identify potential treatments to improve function and accelerate recovery, determine the effica
cy of the identified
treatments in clinical trials in geriatric patients, and Increase the number of junior and senior investigators involved in
geriatric research.

directed by

Elena Volpi, MD, PhD

Dr. James Goodwi
n is the co
. F
or more information on the
current UTMB OAIC, please visit the Pepper website at:

Pepper Center Cores

Research Career and Development Core (RCDC)

The RCDC promotes the development of the next generation of geriatric/gerontologic research leaders through direct
financial support for salary and travel, structured mentoring, partici
pation in interdisciplinary conferences, other didactic
training, networking activities, and access to infrastructure support from the UTMB OAIC, and other OAICs as appropriate.
The RCDC also
provides career development training, supports pilot research p
rojects, and coordinates use of the
research cores by other investigators who are not receiving direct support from the Pepper Older Americans
Independence Center (OAIC).

The Core has two potential targets:

unior faculty and trainees in clinical and ba
sic science disciplines relevant to aging

stablished investigators with expertise highly relevant to aging research but not currently studying aging.

The RCDC takes different approaches to these two groups. For junior faculty, the RDC coordinates struct
ured didactic
training with the provision of appropriate mentoring. Junior faculty are assigned both research mentors and career
mentors, the one to focus on the specific goals of the research project and the other to assist with defining overall career
als. Mentors are selected from among senior faculty in aging research. A major theme of the training program is linked
to mechanistically driven research to evaluation of clinical outcomes. RCDC activities involving more established faculty
concentrate on
providing opportunities and support for interdisciplinary collaboration, drawing investigators from other
disciplines into ongoing studies of the OAIC.

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The RCDC supports investigators
interested in developing a research portfolio in aging from
in a wide
spectrum of clinical
and basic science disciplines

Clinical Research Resource Core (CR

The Clinical Research Core (CR


the primary OAIC
source for
subject recruitment, tracking and
retention activities.

It supports studies related to 1) biological mechanisms underlying muscle loss and re
growth; and 2)
physical function and disability in both healthy community
dwelling older adults and those admitted to our Acu
te Care for
Elders (ACE) unit.
The Specific

Aims and functions of the CR

RC1 are to:


Recruit, track and retain older adults for external projects, developmental studies and pilot studies. Older adults
included in Core activities range from healthy older adults living in the community to those hosp
italized with an
acute illness.


Provide uniform and standardized health screenings, physical exams and functional status and disability
assessments for investigators of external projects, developmental studies and pilot studies. As examples, trained

from our Core will perform phlebotomies, DEXA scans, muscle biopsies, and subjective and objective
assessments of physical function and disability status.


Maintain a health outcomes database on older patients admitted to our ACE unit. Recruitment coordina
tors from our
Core will maintain a password protected web
based database on older patients admitted to our ACE unit, and made
available to OAIC investigators.


Coordinate training in recruitment and retention, and functional assessments, and ensure complian
ce regarding the
regulations governing clinical investigations involving human subjects.

Muscle Biology Resource Core (MB

The Muscle Biology Resource Core (MB
RC2)supports and promotes integrative and translational research on the
biological mechanisms underlying muscle loss and functional recovery in older adults. The specific aim
s are:


Provide analytical support for funded translational research in muscle aging requiring









uscle m


racer methodologies

to measure metabolic processes in vivo


Develop new translational methods to study th
e mechanisms of sarcopenia and muscle functional recovery in older


Simplify access of OAIC investigators to other institutional analytical core resources


Train young investigators on the analytical and methodological aspects of translational muscle

research in older

Pilot/Exploratory Studies Core (PESC)

The goal of the Pilot/Exploratory Studies Core (PESC) is to foster the development of innovative research designed to
foster translation of basic discoveries on t
he mechanisms of muscle loss and re
growth with aging to clinical practice for
the promotion of functional recovery in older adults. Specific aims



Solicit and select the most meritorious research proposals for PESC funding


Provide PESC investigators w
ith access to resources from other OAIC cores and institutional research


Identify potential opportunities for co
sponsorship of PESC studies


Monitor the progress of PESC studies


Ensure regulatory compliance, safety and protection of huma
n subjects enrolled in PESC studies


Provide assistance and mentorship to develop PESC studies into independently funded grant applications