Biotechnology Unit Study Guide
Chapter 13, lab and internet
Biotechnology is a field of biology that uses the latest technologies for manipulating and
investigating living organ
isms. Some of the techniques that fall under biotechnology include gene
therapy, DNA fingerprinting, and genetically modifying organisms (i.e., for agriculture). Our
biotechnology unit also afforded us the opportunity to examine some of the ethical consi
using such technology.
After completing this unit, students should be able to:
Identify some of
the ethical implications of genetic
and explain why it is a useful
Name some of the di
sorders for which gene
therapy is currently being investigated as a
Identify advantages of gene therapy
, in general, the process of using a virus as
for gene therapy.
Define the term “
” or “
Describe some of the
uses of genetically modified/transgenic organisms
steps for creation of a
List the steps of
and know the
barriers that must be broken through in the
nderstand how various
chemicals are involved in this procedure
some common ways that DNA is
obtained from people.
Identify various uses of
Describe the process and steps of DNA fingerprinting (i.e.,
extraction, making an
, loading DNA, gel
, staining or
Identify the charge on DNA and
describe what happens to
DNA fragments during electrophoresis.
Compare DNA fingerprints and
make conclusions based on the
and how they are used in DNA fingerprinting
Relate DNA restriction fragment size to the position of DNA “bands” on a DNA
which fragment in the picture above is largest? smallest?
a restriction enzyme will cut when given the restriction
recognized and one or more DNA sequences. Know the #s and sizes of the fragments.
Predict what a gel will look like, based on DNA sequences and restriction
sites/fragments, and probe seque