GENETIC DIVERSITY ANALYSIS OF SEVEN STRAINS NILE TILAPIA

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23 Οκτ 2013 (πριν από 3 χρόνια και 7 μήνες)

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ABSTRACT



GENETIC DIVERSITY ANALYSIS OF SEVEN STRAINS NILE TILAPIA
(
Oreochromis niloticus
) BY USING MICROSATELLITE DNA
MARKER

By : Wahyu Endra Kusuma
1
; Sukoso
1
, Ratu Siti Aliah
2
.

1

Master Program Marine and Fisheries Biotechnology, Fa
culty Of Marine And Fisheries Science
Brawijaya University, Malang, Malang.
2

Researcher in The Agency of Assessment and Application of
Technology (BPPT)

Decreasing of genetic variability in aquaculture cannot be avoided. In tilapia aquaculture,
decreasing

degree of genetic variability faster than other aquaculture species because of its
overbreed characteristic. Therefore the proper breeding program to improve tilapia genetic
should be carried out. Genetic characterization is very important to be done to s
upport those
programs. Genetic characterization has been conducted through measuring genetic
variability and heterozigosity. Seven strains of tilapia; (NIRWANA (nila strain Wanayasa),
JICA, nila Sukabumi F3 from selected family (SELFAM), BEST, Putih Umbula
n, NIFI and
also JATIMBULAN (Jawa Timur Umbulan) has been analyzed with four loci microsatellite
DNA which is
UNH147*, UNH172*, UNH216*,
and
UNH222*.
Moreover, the GGPS, GPS
and PS of NIRWANA strains also have been monitored and characterize using those
mi
crosatellite loci. The average number of allele and heterozigosity detected from 7 strains
of tilapia were 3,000
-
7,750 and 0,290
-
0,766 respectively. Hardy
-
Weinberg Equilibrium test
shows significant of homozigosity excess. Based on UPGMA dendogram, 7 strai
ns of tilapia
were divided by 4 clusters with the lowest and highest value 0,1467 (SELFAM
-
Nila Putih
Umbulan) and 0,6018 (NIRWANA
-
JATIMBULAN) respectively. From NIRWANA strain
(GGPS, GPS and PS population) the average number of allele and heterozigosity de
tected
were 3,000
-
4,750 and 0,438
-
0,563 respectively. SELFAM strain can be considered as
alternative candidate of genetic variation source because SELFAM has highest
heterozigosity than the other strains. Hybrid offspring with superior characteristic could

be
produced from crossbreeding between JICA, NIRWANA and JATIMBULAN strain. While
decreasing of genetic variability in NIRWANA strain GGPS, GPS and PS population was
might because of lacking number of samples that used in this observation.

Keywords; tila
pia, microsatellite DNA marker and genetic variability