Course No - TERI University

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Course No.:





BBT 151

Course title:





Environmental Biotechnology

Number of credits:




3 (2.25
-
0
-
0.75)


No. of lectures
-
tutorial
-
practical:


32
-
0
-
20

Course coordinator:




Dr Anandita Singh




Course outline

The present course seeks to bring about a

conceptual understanding on key issues related to
Environmental Biotechnology. The major focus will be on the impact of microbial and
agriculture biotechnology on environment. The course has been structured as two distinct
modules. The first module aims t
o create awareness about the role that microbial
biotechnology promises to play in relation to bioremediation. The relevant topics include
introduction to basic microbiology, principles of microbial genetics and molecular biology.
The course will provide a
n in
-
depth knowledge on aspects of biotransformation and bio
-
degradation of specific pollutants, removal of pollutants from the environments and
bioremediation of polluted lands or water bodies. The second module is intended to provide
an exposure to diver
se biotechnological practices for sustainable agriculture. An introduction
to plant genetic transformation will be provided to highlight both the positive and negative
impact of transgenic technology on environment. The students will be sensitized to the
e
nvironmental biosafety issues related to GMOs, the regulatory issues including the
regulatory framework of India. Socio
-
economic and ethical aspects of releasing GMOs in the
market will also be covered. The course will also sensitize the students to how bi
otechnology
and various molecular biology techniques address the issues pertaining to management of
germplasm resources for conservation. The aim of the course will be both to understand the
basic concepts in environmental biotechnology as well as applicat
ion of biotechnology for
addressing environmental problems.


Evaluation procedure



Class discussion and Practicals

: 15 %



Tutorials/assignment


: 15 %



2 minor tests



: 30% (15% each)



1 major test (end semester)

: 40%


Details of course content & allotted t
ime


S.No

Topic

Allotted time (hours)

Lecture

Tutorials

Practicals

Module 1


Microbiology, Biotransformation
and Biodegradation





Introduction to microbiology and its
applications, Prokaryotic cell structure
and function, Microbial genetics:
Recombi
nomics, genes and their
organization, genetic code, gene
expression and regulation.

4



S.No

Topic

Allotted time (hours)

Lecture

Tutorials

Practicals


Techniques of isolation, enrichment,
purification and characterization of
microbes, Methods for identification
of microbes biochemical test, FAME
analysis, 16s rDNA s
equence,
pyrolysis mass spectroscopy
techniques of preservation of
microbes.

3





Current issues in environmental
pollution and environmental
protection: Bioremediation of
industrial solid and liquid wastes and
its mitigation, Biodegradation
properties,
Detoxification of metals by
microbes, Biodegradation of oil sludge

3




Methods in determining
biodegradability, Use of microbes in
biodegradation and biotransformation
of pollutants

2




Monitoring of bioremediation,
Bioindicators and biosensors for
env
ironmental monitoring, Recovery
of natural resources (oil recovery,
biobleaching)

2




Practical:





Culture methods, selective and
differential media, media preparation.
Pure culture, culture handling and
preservation.



7


Isolation, purification
and
characterization of microbes,
microscopy and staining



7

Module 2


Biotechnology for sustainable
agriculture and conservation of
genetic resources





Transgenic plants for sustainable
agriculture: Imparting tolerance to
abiotic and biotic stresses,

Herbicide
tolerant transgenics, Phytoremediation
and Nutritional enhancement

2




Principles of plant genetic
transformation (Tools, techniques and
methodologies)

1




Impact of modern biotechnology on
biodiversity and environment: Lateral
gene flow, Re
levance of Biosafety and
Biotech Regulations, Regulatory
Framework of India

4



S.No

Topic

Allotted time (hours)

Lecture

Tutorials

Practicals


Socio
-
economic and ethical issues
concerning GMOs in agriculture and
industry: IPRs and best business
practices in Agbiotech research, Case
studies

3




Biotechnology for ge
rmplasm
conservation and management of
PGRs: PGRs as a source of novel
genetic variation, introduction to
molecular markers, DNA
fingerprinting for assessment of
genetic diversity and germplasm
management

4




Integrated Plant Nutrient Management
(Soil Mi
crobiology: Bio

fertilizers and
for sustainable soil health and crop
productivity)

inorganic, organic
fertilizers)

2




Integrated Pest Management:
Concepts, Definitions, Approaches
and Relevance

2




Practical:





Introduction to basic plant molecula
r
biology techniques: PCR technique for
GM detection, ISSR fingerprints,
Agarose gel electrophoresis and
visualization on ethidium bromide
staining



6


Field trip to NBPGR: Principles of
in
-
situ

and
ex
-
situ

conservation and
management of plant genetic
re
sources



0


Total

32


20


Basic textbooks


1.

Manual of Environmental Microbiology (2nd edition) Editors: Hurst CJ, Crawford
RL, Knudsen GR, MchInerney MJ, Stetzenbach LD (2002) ASM Press

2.

Bioremediation of contaminated soil. Editors: Wise DW, Trantolo DJ
, Cichon EJ,
Inyang HI, Stottmeister U (2000) Press

3.

Manual of Industrial Microbiology and Biotechnology (2nd Edition) Editors: Demain
AL and Davies JE, Atlas RM, Cohen G, Hershberger CL, Wei
-
Shou Hu,. Sherman
DH, Willson RC and David Wu JH (Year) Press

4.

Pri
nciples of Biochemistry (3rd edition) Authors: Nelson and Cox (2003) Lehninger,
Macmillan press

5.

Applied plant biotechnology. Authors: Chopra VL, Malik VS and Bhat SR (1999)
Oxford University Press

6.

Environmental pollution and control (4th edition) Authors
: Peirce, Weiner and
Vesilind (1998) Butterworth
-
Heinemann

7.

Biotechnology and safety Assessment (2nd edition) Authors: Thomas (1999) Replika
Press Pvt. Ltd.

8.

Environmental microbiology. Authors: Varnam and Evans (2000) ASM press

9.

Practical Applications of Pla
nt Molecular Biology. Authors: Henry RJ (1997)
Chapman & Hall

10.

DNA fingerprinting in Plants and Fungi. Authors: Weising K, Nybom H, Wolff K and
Meyer W (1995) CRC Press, Boca Raton

11.

Core Collections of Plant Genetic Resources. Authors: Hodgkin T, Brown AHD,
Hintum Th. J.L. van and Morales EAV (1995) John Wiley & Sons

12.

Principles of Genetic Manipulation (6
th

Edition) Authors: Primrose SB, Twyman RM
and Old RW (2001) Blackwell Science Ltd, MA 02148
-
5020, USA

13.

Plant, Genes and Crop Biotechnology (2nd Edition) Auth
ors: Chrispeels MJ, Sadava
DE (2003) Jones and Bartlett Publishers, Inc. London, UK

14.

The biology of microorganisms (8
th

edition) Authors: Brock TD, Madigan MT,
Martinko JM and Parker J (1994)
909 Prentice Hall


Other suggested readings


1.

Soil Microbiology,
DJ Bhagyaraj

2.

Soil Microbiolgy, RR Misra

3.

Advances in Agriculture Microbiology, NSS Rao

4.

Soil

reclamation

processes:

Microbiological

analyses

and

applications, RL Tade and
AK Donald

5.

Microbiology by L M Prescott, J H Harley and D A Klein

6.

An In
troduction to Microbiology by Barry

7.

Biochemistry and molecular biology of plants. Authors: Buchanan BB, Gruissem W
and Jones RL (2000), American Society of Plant Physiologists

8.

Introductory Plant Biology. Stern KR (1997) WM Brown publishers

9.

Useful Web site
s/notes/handouts (to be provided by instructors)