Microbio10new.pptx

triteritzyΒιοτεχνολογία

14 Δεκ 2012 (πριν από 5 χρόνια και 23 μέρες)

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Biopolyester Particles

The principal idea is:

Novel plastic material


The Idea is:


Exposure over 45 days:

Inclusions in MOs


Inorganic Inclusions:


Magnetosomes



Organic Inclusions:


Biopolyester granules

(PHA)

Production of Inclusions


The key enzymes are the polyester synthases



PHA synthases:


These enzymes catalyse the enantioselective
conversion of (R)
-
3
-

hydroxyacylCoA substrates to PHAs


In this process CoA is released again


88 synthases are cloned and characterised



PHA producing bacteria

Production of Inclusions


PHAs are produced:


When Carbon sources are available in surplus


When other nutrients are limiting


PHAs are stored in the cells as water insoluble
particles inside the cell.


Who is producing PHAs:


Eubacteria


Archea

Why do these bacteria produce PHAs?


PHAs are produced as intracellular storage


Upon carbon source starvation the polymers
are being mobilised again


Enzymes used for this:


PHA Depolymerases


Location of the enzymes:


On the surfaces of the granules

PHA metabolism and its macro
regulation

Important carbon sources for growth
and PHA production

Biopolyester

PHAs potential applications


Size, core composition and surface
functionality can be taylored


Functionalised nanoparticles


Biocompatible, biodegradable


Drug delivery


Protein immobilisation


diagnostics

PHAs applications


Thermoplastic properties


Packaging industry


Medicine


Pharmacy


Agriculture


Food industry


Raw material for enantiomerically pure
chemicals


Structure and Properties of 2 Major
PHAs

The 4 Main groups of PHA synthase

Genetics of PHA synthases

Polyester synthese

Metabolic routes for Polyester
biosynthesis

Polyester inclusion self assembly and
structure

Taylor made Biopolyester particles

PHA production

Production of fusion proteins

PHB chips for immunoassays


µContact Printing

Micro patterning

Long term stability of printed fusion
protein

Confocal image of Fusion protein µCP
onto PHA surfaces

PHA coated SPR chips

41

© 2005 Nokia YN
10.10.2006

Trends for Plastic Materials


Raw Material shortage


Polymers from renewable raw materials will become important


Current examples like


PHA (
polyhydroxyalkanoate
) grown in genetically modified corn plant leaves


PLA (
polylactide
) produced by the fermentation of sugars extracted from
plants


PHB (
polyhydroxybutyrate
) produced by bacteria.


New synthesis methods of old polymers like PA11 will be established :
example PA11 derived from castor plant

based renewable resources


Protein polymers


Extreme mechanical properties


Protein polymers are synthetic proteins created "from scratch" through
chemical DNA (gene) synthesis, and produced in quantity by traditional
large
-
scale microbial fermentation methods


Through genetic engineering, it will be possible to tailor the physical
structure and biological characteristics of protein polymers to achieve
required properties


Due to their synthetic design, protein polymers are capable of
combining the biological functionality of natural proteins with the
chemical functionality and exceptional physical properties of synthetic
polymers

Materials technology in a key role


Materials technology as a potential
enabler for:


Enhanced user experience


New functionality


New form factors


Improvements in production efficiency


New solutions for energy management,
data storage


Need multi
-
disciplinary research


materials, mechanics, memory,
electronics, energy


Considerations for environmental
sustainability,

volume production


43

© 2005 Nokia YN
10.10.2006

Trends for Plastic Materials


Tailoring of properties is made through additive technologies


Old property fine tuning with additives like internal lubrication,
thermal conductivity, and static dissipation


smart plastics with additives


Tunable electrical properties


Polymer magnets


Shape memory plastics


Tunable friction properties


Nano

Technologies





Biodegration


Controlled biodegradation will be used in many new applications


Food preservation


Explosives


Security