Biotechnology and Genetic
Engineering
15.3
A.K.A.
Modifying
Organisms to
Fit
Human Needs
Cloning A Gene
Make an exact copy of a gene
Why is Bacteria widely used to clone genes?
What do scientists want to clone genes?
•
http://
www.youtube.com/watch?v=GNMJBMt
KKWU&feature=related&safety_mode=true&p
ersist_safety_mode=1
(plasmid)
•
http
://
www.youtube.com/watch?v=TpmNfv1j
KuA&NR=1&feature=fvwp&safety_mode=true
&persist_safety_mode=1
(RDNA)
Creating Genetically
Modified/Engineered Organisms
How do you Genetically Modify an
Organism?
Step 1: Combine Genes from different organisms
*Key Term:
Recombinant DNA*
How do you genetically modify an
organism?
Step 2: Transfer Recombinant DNA into host Cell
*Key Term:
Transformation
*
The Green Gene Lab
•
Objective: To see if
pGLO
gene is successfully
transferred
The Green Gene Lab
•
Organism to be modified:
E.coli
•
Transforming the
E.coli
with
pGLO
Remember….Transformation is never 100%!!!
Overview of the Procedure
Easy way to see if the Bacteria got
Transformed with
pGreen
!!
NO PLASMID ADDED
pGLO
PLASMID ADDED
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