E.coli - MsVentrella

Biotechnology and Genetic
Engineering

15.3

A.K.A.



Modifying

Organisms to
Fit
Human Needs

Cloning A Gene

Make an exact copy of a gene

Why is Bacteria widely used to clone genes?


What do scientists want to clone genes?

•
http://
www.youtube.com/watch?v=GNMJBMt
KKWU&feature=related&safety_mode=true&p
ersist_safety_mode=1

(plasmid)


•
http
://
www.youtube.com/watch?v=TpmNfv1j
KuA&NR=1&feature=fvwp&safety_mode=true
&persist_safety_mode=1

(RDNA)


Creating Genetically
Modified/Engineered Organisms

How do you Genetically Modify an
Organism?

Step 1: Combine Genes from different organisms

*Key Term:
Recombinant DNA*

How do you genetically modify an
organism?

Step 2: Transfer Recombinant DNA into host Cell


*Key Term:
Transformation
*

The Green Gene Lab

•
Objective: To see if
pGLO

gene is successfully
transferred

The Green Gene Lab

•
Organism to be modified:

E.coli

•
Transforming the

E.coli

with
pGLO

Remember….Transformation is never 100%!!!

Overview of the Procedure

Easy way to see if the Bacteria got
Transformed with
pGreen
!!

NO PLASMID ADDED

pGLO

PLASMID ADDED