Exercise 10

tomatoedgeΒιοτεχνολογία

20 Φεβ 2013 (πριν από 4 χρόνια και 5 μήνες)

113 εμφανίσεις

10…

MICROB
I
AL

GENET
I
CS

AND

BIOTECHNOLOGY


The goals f
o
r this exe
r
cise ar
e
:



to provide
p
r
a
c
tice in

sel
e
c
t
ing, perf
o
rmi
n
g, and inter
p
reting bi
o
c
h
e
mical tes
t
s



to provide
p
r
a
c
tice in
u
s
i
n
g the refere
n
c
e

materia
l
s fo
u
nd in

Virtua
l
Unk
n
own™

M
icrobiology



to

con
n
ect t
h
e disc
u
ss
i
on

of molecu
l
ar

manipu
l
atio
n
s

and b
i
otec
h
nology in l
e
cture with the
o
rgan
i
s
m
s in

VirtualU
n
k
n
own™
M
icrobiology
,

their habitats, and

their traits



to provide
p
r
a
c
tice in
u
s
i
n
g test r
e
s
u
lts f
o
r the purp
o
se

of identifying bacter
i
a


Instructions
:


1.

Enter the Virtual Lab
a
nd select
Bi
o
te
ch
nolo
g
y

from the list of pre
d
efined u
n
k
no
w
n
s
.

For this exerc
i
se ch
e
ck
the box to enable aut
o
-
in
o
c
ulati
o
n to s
p
eed up the

work
o
n

this e
x
erc
i
se.

Use
t
his predefi
ne
d unkn
o
wn f
o
r
your w
o
rk on

the
followi
n
g prob
l
em:

You have b
e
en hir
e
d by a

biotech firm

to find new i
n
s
e
ctic
i
d
e
s that are
m
ore e
n
v
i
ron
m
ental
l
y
-
friend
l
y.
You beli
e
ve there
a
re

safe

and effective

ways to ge
n
e
t
ical
l
y
-
e
ngi
n
eer nat
u
ral fl
o
ra of p
e
st insects to
pro
d
uce a r
e
cently d
i
scovered
insec
ti
c
id
a
l protein. Always lo
o
k
ing f
o
r potential b
a
cterial
h
os
t
s for the
toxic
g
ene, you h
a
ve call
e
d yo
u
r old c
la
ss
m
at
e
s
.

One, an env
i
ro
nm
ental
m
icrobiolog
i
st w
o
rking for
NASA, sent this as a cand
i
date. Use t
h
e
m
edia and tests at your disposal in
VirtualUn
k
now
n

M
icrobiology
to identify this u
n
k
n
own org
a
nis
m
.
What is its identit
y
?









2.

Would

y
ou
s
ugge
s
t th
a
t this microbe has ec
o
nomic po
t
e
n
tial for gen
e
tic
e
ngineering
to control p
e
st
insects
?
Explain
.




















3.

If

y
ou found the or
g
ani
s
m

to ha
v
e p
r
omise as a
v
ector

to int
r
oduce a tox
i
n gene into target in
s
ects,
w
ha
t

w
o
uld be its potential stren
g
ths

and its pot
e
ntial

w
e
a
k
n
e
sse
s
?
























45

B
as
ic Tr
a
ining

M
a
nu
a
l

for
VUMicro™

3.11

Name:
_



Co
p
y
right

©

20
0
3
-
2005,

In
t
uitive

S
y
stems,

Inc.

Section:
_





4.

Whet
h
er
o
r
n
ot this org
a
n
i
sm shows
pr
omise, you

know introd
u
c
t
ion of the toxin gene into i
t
s
ge
n
ome is possib
l
e.
Pro
v
ide an experimental p
l
an to intr
o
d
u
ce the

toxin

gene, n
o
w

f
o
und in a Gr
a
m

negati
v
e bacillus, ex
p
ressed
b
y

the bacterium just ident
i
fie
d
.


GOAL OF
TH
E PROJ
E
CT:











METHODS AND SEQUENCE TO BE USED:
































HOW GENE

EXPRESSION W
O
ULD
B
E

MONITORED:




















HOW EFFECTIVENESS

WOULD BE DETERM
I
N
E
D: