www.phy.syr.edu/courses/PHY106/PHY106.01Spring/Sli...

thelemicbathΒιοτεχνολογία

20 Φεβ 2013 (πριν από 4 χρόνια και 6 μήνες)

136 εμφανίσεις

Molecular Biology &

Genetic Engineering

Polymerase Chain Reaction


Method for amplifying DNA segments


thermal cycling (with
Taq

polymerase)



denaturation & renaturation


Amplification: 1, 2, 4, 8, 16, …


Dr. Kary Mullis, 1983 (then at Cetus)


Nobel Prize 1993


PCR animation

(Shockwave)


Cycle sequencing animation
(Shockwave)



DNA Fingerprinting animation

DNA Fingerprinting with VNTRs*

*VNTR = Variable Number of Tandem Repeats

Hormone

Stem cells


embryonic
vs.
adult stem cells


tissue
engineering


cell therapies


transplants


somatic
-
cell
nuclear transfer



DNA Chips (DNA microarrays)


Visualize gene expression
from a given cell/tissue type


Hybridize fluorescence
-
labeled cDNA with probe
DNA on the chip and analyze
array patterns


Chips are made using
“photolithography” as in
manufacture of integrated
circuits for microelectronics.


DNA
chips
used to
identify

cancer
types

Additional Topics & Buzzwords


functional genomics


sequences, functions, regulation/control, …


proteomics


analyze proteins instead of genes (genomics)


bioinformatics


information technology & molecular biology


systems biology


structures, behaviors, control, design, …




Biotechnology:
Present and
Future