Services – Microbiology & molecular biology - Degain Group

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20 Φεβ 2013 (πριν από 4 χρόνια και 5 μήνες)

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About Us


Company Profile

Skanda Life sciences Pvt ltd is carrying out research and development in the areas of
Biotechnology, drug discovery, natural products, chemistry & herbal formulations. The
organization aims to facilitate, encourage, aid, promote,

and engage in innovative scientific
research in Life sciences and related fields. The objective of this organization envisioned a
corporation that would collaborate and work in close partnership with various research
institutes, government and private ind
ustries.

Our research services provide the following areas.



Chemistry/ Phytochemistry



Biology (Drug Discovery)



Microbiology & Molecular biology



Preclinical Studies



Herbal Formulations & Development



Marketing of phytochemicals



HR Placement Services

Vision



Will be provided later


Mission

Will be provided later


Services in brief



We undertake Student Projects / Dissertation for BE (Biotech), M.Sc., M.pharm, MBBS, MD
and Ph.D. using well validated methods /protocols/SOPS.



Short term courses in
cell Biology/Chemistry/Animal Studies



ADME studies/PK studies



Collaborative Project proposals, Experiment planning, Data interpretation/Project report
submission



Consultancy: Placement in Life Sciences and clinical Trials



Herbal formulation and marketing
.



Services


Chemistry & Phytochemistry


Chemistry & Phytochemistry

Chemistry


The chemistry division has experienced chemist capable of delivering custom synthesized NCEs,
tailor made compounds, derivatives, analogues, standards & APIs


Skanda Life S
ciences provide the following services:



Hit & lead development in various therapeutic areas such as Antifungal, Oncology,
Metabolic disorders, AD in drug discovery programs



Chemistry services integrated with in
-
vitro and in
-
vivo in biological profiling



Structure based designing of compound libraries



Route identification, process optimization, custom synthesis & formulation studies on lab
scale



Process analytical development & Product development



Sourcing & marketing chemical entities


Phytochemistry



This sector is focused on development of plant
-
based drugs through investigation of leads.
Plants provide an extensive reservoir of natural products, demonstrating significant structural
diversity and offer a wide range of
new molecules. We manufacturer and supply of herbal
extracts, phytochemicals, veterinary healthcare products and Cultivation of medicinal plants

Skanda Life Sciences is focused on novel product development, herbal extraction, isolation,
identification and
purification of bio
-
active molecules. We offer the following services:



Isolation of plant compounds based on bioactivity guided fractionation



Process development for extraction procedures (refluxation, soxhlation) for medicinal plants



Development of phyt
ocompounds using different techniques by chromatography methods




Screening of standardized extracts, natural products & plant molecules in
-
vitro & in
-
vivo
biopharmacological assays for Antioxidants, Antidiabetic, Anticancer, Antidepressant, Anti
-
inflammato
ry, AD & Osteoporosis



Stability studies for the finished, formulated health herbal products



Organization cultivating Coleus forskohlii in 25 acres in Mysore region (180km from
Bangalore)

Services
-
In Vitro Biology


In vitro Biology

The
In Vitro

Biology group performs a variety of biochemical and cell based assays against target
enzymes, GPCR’s, and
transporters. We

developed methodologies, which is acceptable as per USFDA
on target validation and mechanism studies




Biochemical & cell based assay

development & validation



Statistical support



Algorithms for data processing and quality evaluation



Assay Development & Validation (In
-
vitro)



Assay Formats/ Technology/Platform



Cell based ELISA



FRET, TRF,
HTRF



Filter Binding, Kinetic Assays,



SPA assays



GPCR assays Ca2+ and cAMP
based assays



hERG Assays



Whole Blood Assays



Cell Viability (LDH, MTT, Alamar
Blue)



Flow Cytometry Assays*



Western Blotting and In Cell ELISA


Validated assays
;



Screening assays for detection and measurement of drug induced apoptosis & TUNEL
assay, Cytotoxicity evaluation of the test compounds by MTT &

Alamar blue reduction,
Cell cycle analysis, drug induced Apoptosis by FACS based assays.



COX I & II, Hyaluronidase & Protease assay, Mast cell stabilizing assays



Alpha Amylase & Disaccharidase (Glucosidase) inhibition assay, Aldose reductase
assay, DPP

IV inhibition assay, Aldolase assay, PTP 1B inhibition assay, Insulinogenic
activity of test compounds using RIN m5F cells , GPCR targets based cell line
development.



Acetyl & butyryl cholinesterase assay (AD), Nitric oxide scavenging assay, DPPH,
Sup
eroxide dismutase, hydroxyl radicals assays (antioxidant)



Assay Technologies to be outsourced




Radiometric
-

SPA, Filter binding, Flash plate



Fluorescent
-

FP, FRET, HTRF, TRF, DELFIA, Lance



Calcium based assays



Luminescent based assays


Services


Microbiology & molecular biology


Microbiology & Molecular Biology

Microbiology


Application of molecular biology tools in microbiology has made rapid advances in methods for
studying microorganisms. We in conjunction with conventional
microbiological techniques
have adapted molecular tools for rapid identification & characterization of microorganisms. We
offer following services



Characterization of microorganism by molecular approaches (16SrRNA, 18S rRNA, ITR gene
analysis, RAPD, RFLP,

gene specific PCR.



Microbial metabolites testing (Endotoxin, aflatoxins)



Pathogens detection as per WHO & FIP [E.coli, Salmonella, Pseudomonas, Klebsiella
pneumonia, Staphylococcus aureus, streptococcus sp., Mycoplasma sp.,



AMES Mutagenicity test



Disinfectant efficacy testing, MIC determination, Residual antimicrobial substances testing.




Products development for antibacterial & antifungal therapy


Molecular Biology

Molecular biological tools have given great insights in to biology of the underlying diseases
helping scientist in identifying new therapeutic targets. At Skanda Lifesciences scientists strive
to develop stable recombinant proteins expressing cell lines fo
r application in drug discovery
research.


Services



Gene cloning and gene expression studies



Recombinant proteins expressing stable cell line development.









Services
-

Drug Metabolism and Pharmacokinetics (DMPK)


Drug Metabolism and
Pharmacokinetics (DMPK)



Skanda groups developed a validated permeability studies Caco2 & MDCK cells

with
basic facility &
expert

scientific team.




DMPK team supports both
integrated preclinical

discovery programs and fee
-
for
-
service projects and
delivers quality and reproducible results with a quick turnaround time. DMPK basically comprises in vitro,
in vivo, met
-
ID and pre
-
formulation groups managed by experienced and scientific people.
This di
vision
guides
the discovery projects by trouble shooting the scientific issues and offer changes on scaffolds to
drive the discovery projects in a right direction
. Our cost is competitive & good quality results to be
expected.


ADME
in brief

Physicochemical properties



Solubility (Thermodynamic and Kinetic
method)




Log D



Chemical Stability



Blood Partitioning


Permeability
studies



PAMPA



Caco
-
2 & MDCK


Animal Pharmacokinetics


Pharmacokinetics by various routes
viz
., Oral,
I
ntravenous,
I
ntraperitonial, subcutaneous in mice
(Swiss Albino, C57, Balb/C), rats (Wistar, Sprague
Dawley), rabbits (New Zealand white),




Tissue Distribution



Brain to plasma ratio



Mass Balance


Metabolism
studies



Cytochrome P450 inhibition



Cytochrome P450 time dependent
inhibition


Metabolic Stability




Liver Microsomes, S
-
9 fraction,
Hepatocytes Identification in vitro
(microsomes, S9 and hepatocytes) & in
vivo samples




Plasma stability



GSH trapping


Protein Binding



Plasma protein binding (ultra filtration and
equilibrium dialysis)



Microsomal protein binding




Services


Custom research & Placements


Custom Research

Biotechnology



Biofuel Research/
Downstream processing



Monoclonal antibody /Biosimilars



Research oriented methodologies to ensure accuracy and timely release of reports.



Chemical analysis of test material (Raw material), food material, water, syrups, tablets or
any pharmaceutical samples

Metal detection, Total organic carbon, Sulfate, Nitrogen
estimation, Total residual chlorine, Calcium, Magnesium, total solids etc Effluent sewage
treatment, BOD, COD, Dissolved oxygen tests


Environment Projects:



Contracting environmental studies with v
arious industries and Pollution control board like
sewage management & treatment, Air monitoring, chemical analysis, pollutants analysis,
pollution controlling in air, water & soil.

















Products


Herbal Formulations & Marketing


Herbal
Formulations & Marketing


Formulation and development is key for maintaining quality of active ingredients during
product life cycle of herbal preparation. Our scientific team has expertise in development of
appropriate dosage forms to maximize product qua
lity throughout the product lifecycle.

Services:



Veterinary & Human Herbal formulations



Development of stable dosage forms



Pilot scale to commercial Scale up trials



Marketing of herbal products / Phytochemicals



Phytochemicals reference standards.



















News & Events

Will be provided later

























Contact Us

Office:


#251, 3rd main,

Jagajyothi layout,

Nagadevenahalli,

Bangalore
,

Karnataka, India

PIN
-

560056



R&D centre:


#767,

Near Infosys, Industrial area,

Hootagalli,

Mysore

Karnataka, India

PIN
-

571186




Email:

info@skandalifesciences.com

bd@skandalifesciences.com



Telephone:


+91 9738375537

+919632280882