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thelemicbathΒιοτεχνολογία

20 Φεβ 2013 (πριν από 4 χρόνια και 8 μήνες)

127 εμφανίσεις

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Adaptation of nitrifying microbial biomass to nickel in batch incubations

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Supplementary Materials

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Chok
-
Hang Yeung
a
, Christopher A. Francis
b
,
Craig S. Criddle
a

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a

Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305,
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USA.

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b
Department of Environmental Earth System Science, Stanford University, Stanford, CA 94305,
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USA.

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*

Corresponding author: Chok
-
Hang Yeung

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Mailing

Address:

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Department of Civil and Environmental Engineering

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Stanford University

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The Jerry Yang and Akiko Yamazaki Environment and Energy Building

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473 Via Ortega

22

Stanford, CA 94305
-
4020

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USA

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E
-
mail: chyeung@stanfordalumni.org

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Running title:
Adaptation of

nitrifying biomass

to nickel

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Submission as a full research paper to Applied Microbiology and Biotechnology

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SUPPLEMENTARY TABLES

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Table S
1
. Multiple pairwise comparisons of AOB
amoA

T
-
RFLP
profiles
for nickel level
2

with P values
shown. (
P
<0.05 were indicated in bold).

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Nickel Level

Control

1 mg/L

5 mg/L

10 mg/L

50 mg/L

Control






1 mg/L

0.6922





5 mg/L

0.0982

0.1184




10 mg/L

0.0640

0.0570

0.8180



50 mg/L

0.0016

0.0014

0.0854

0.1078


150 mg/L

0.0004

0.0002

0.0006

0.0006

0.0004


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Table S
2
. Multiple pairwise comparisons of AOB
amoA

T
-
RFLP
profiles
for exposure time
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with P values shown. (P<0.05 are indicated in bold)

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Time

0

2 weeks

4 weeks

6 weeks

0





2 weeks

0.5986




4 weeks

0.1136

0.3760



6 weeks

0.0002

0.0002

0.0002


8 weeks

0.0002

0.0002

0.0002

0.0380


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10

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SUPPLEMENTARY FIGURE
S

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Fig
.

S1

P
articulate organic nitrogen (PON)
concentrations in (
a
) control, and

batch cultures exposed to (
b
)
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10 mg/L and (
c
) 150 mg/L nickel.

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5

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0
20
40
60
80
100
120
140
160
180
200
0
1
3
7
10
14
28
42
56
Particulate Organic Nitrogen
Concentrations (mg N/L)

Time (days)

0
20
40
60
80
100
120
140
160
180
200
0
1
3
7
10
14
28
42
56
Particulate Organic Nitrogen
Concentrations (mg N/L)

Time (days)

0
50
100
150
200
250
0
1
3
7
10
14
28
42
56
Particulate Organic Nitrogen
Concentrations (mg N/L)

Time (days)

(a)

(
b
)

(
c
)

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Fig. S
2
Aqueous nickel concent
rations for batches with added nickel concentrations of (a) 0 mg/L
1

(Control), 1 mg/L, 5 mg/L and 10 mg/L; and (b) 50 mg/L and 150 mg/L. Average values of each
2

duplicate cultures are shown.

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0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
0
10
20
30
40
50
60
Aqueous Nickel Concentrations (mg/L)

Time (days)

Control
1 mg/L
5 mg/L
10 mg/L
0.0
20.0
40.0
60.0
80.0
100.0
120.0
140.0
0
10
20
30
40
50
60
Aqueous Nickel Concentrations (mg/L)

Time (days)

50 mg/L
150 mg/L
(a)

(
b
)