报告题目:Challenges and Opportunities of Genetic Engineering ...

taxmanstrongΒιοτεχνολογία

11 Δεκ 2012 (πριν από 4 χρόνια και 8 μήνες)

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Dr. Duan has been working in the field of plant
biotechnology for many years.
Currently
Dr. Duan
is
the
molecular team 2 leader in J. R. Simplot company's Plant
Sciences Business, working on research and development
of a number of potato agronomic and con
sumer traits using
intragenic and cisgenic technology.
Dr. Duan obtained his
PhD degree from the
University of Connecticut
, where he
w
as a key developer of

the
Gene
-
Deletor technology in Dr.
Yi Li's lab
.

Then he did his post
doctoral

research with
Dr.
Mary

Schuler

at
the
University of Illinois
,
as a key player
in

an
NSF 2010 project, functional genomics of plant P450
g
enes
.
Dr. Duan h
as more than 25 publications appearing in
journals such as Plant Physiology, Arch. in Biochemistry
and Biophysics, Plant Biot
echnology Journal, PROTEINS,
RNA, Photochemistry Reviews and Transgenic Research
.

In this talk he will present his recent work and his point of
view on genetic
engineering
.


Publications


Li, Y and
Duan, H

(2011) Molecular approaches for transgene contain
ment and their potential applications
in horticultural crops. In: Transgenic horticultural crops: challenges and opportunities. Mou. B
(ed.) CRC Press (in press)

Chen, Y., Lu, L., Yang, X.,

Duan,

H
., Deng, W., McAvoy, R., Zhao, D., Smith, W., Thammina, C.
, von
Bodman, S. and Li, Y.
(2008) Progress report of production of sterile cultivars of euonymus.

Acta
Horticulturae. 769: 232
-
239.

Rupasinghe, S.,
Duan, H.,

Frericks
-
Schmidt, HL., Berthold, DA., Rienstra, CM and Schuler, MA (2007)
High
-
yield expression
and purification of isotopically labeled cytochrome P450 monooxygenases
for solid
-
state NMR spectroscopy.
Biochimica et Biophysica Acta
1768: 3061
-
3070

Luo, K.,
Duan, H.,
Zhao, D., Zheng, X., Deng, W., Chen, Y., McAvoy, R., Jiang, X., Wu, Y., Pei, Y and
Li
, Y (2007) A highly efficient system to delete all functional transgenes from pollen of tobacco
plants.
Acta Horticulturae.

764: 21
-
31.

Rupasinghe, S.,
Duan, H
and Schuler, MA (2007) Molecular definitions of fatty acid hydroxylase in
Arabidopsis thaliana.
PROTEINS: Structure, Function, and Bioinformatics

68: 279
-
293

Zheng, X., Deng, W., Luo, K.,
Duan, H.
, Chen, Y., McAvoy, R., Song, S., Pei, Y and Li, Y (2007) The
cauliflower mosaic virus (CaMV) 35S promoter sequence alters the level and patterns of activi
ty of
adjacent tissue
-

and organ
-
specific gene promoters.
Plant Cell Reports.
26: 1195
-
1203

Luo, KM.,
Duan, H.,
Zhao, DG.,
Zheng X, Deng W, Yongqin Chen, Y., Stewart, CN., McAvoy, R., Jiang,
X., Wu, Y., He, A., Pei, Y and Li, Y
(2007) ‘
GM
-
gene
-
deletor’: fu
sed loxP
-
FRT recognition
sequences dramatically improve efficiency of FLP or Cre recombinase on transgene excision from
pollen and seed of tobacco plants.
Plant Biotech. J.

5: 263
-
274

Duan, H
and Schuler, MA (2006)
Heterologous expression and strategies f
or encapsulation of membrane
-
localized plant P450s.
Phytochem. Reviews

5: 507
-
523

Schuler, MA.,
Duan, H
., Metin, B and Ali, S (2006)
Arabidopsis

P450s through the looking glass: a
window on plant biochemistry.
Phytochem. Reviews

5: 205
-
237

Chen, Y.
,
Lu, L.
,
Deng, W.
,
Yang, X.
,
McAvoy, R.
,
Zhao, D.
,
Pei, Y.
,
Luo, K.
,
Duan, H
.
, Y. Li (2006)
In
vitro regeneration and Agrobacterium
-
mediated genetic transformation of
Euonymus alatus
.
Plant
Cell Reports
. 25: 1043
-
1051

Duan, H
., Pei, Y., McAvoy, RJ., Zhao, D., Che
n, Y., Luo, K., Smith RW and Li, Y(2006): Auxin,
cytokinin and abscisic acid: biosynthetic and catabolic genes and their potential applications in
horticultural crops.
J. of Crop Improvement
. 18: 347
-
364

Li, Y., Cheng, Z., Smith, RW., Ellis, D., Chen, Y.,
Zheng, X., Pei, Y., Luo K., McAvoy, R.,
Duan, H
.,
Thammina, C and Zhao, D. (2006) Gene transfer approaches to neutralize invasiveness of exotic
ornamental plants.
J. of Crop Improvement
. 17: 279
-
301

Xiong, A., Yao, QH., Peng, RH.,
Duan, H
., Li, X., Fan, HQ
., Cheng, ZM and Li, Y (2006) PCR
-
based
accurate synthesis of long DNA sequences.
Nature Protocols

1
:
791
-
797

Duan, H
.,

Huang, MY., Palacio, K and Schuler, MA (2005) Variations in CYP74B2

(hydroperoxide
lyase) gene expression differentially affect hexenal
signaling in the Columbia and
Landsberg ecotypes of
Arabidopsis

Plant Physiol.

139: 1529
-
44

Duan, H
., and Schuler, MA.
(2005) Differential expression and evolution of
Arabidopsis
CYP86A subfamily.
Plant Physiol.

137: 1067
-
81.