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22 Φεβ 2013 (πριν από 4 χρόνια και 5 μήνες)

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Zabolotny

Institute of
Microbiology and Virology of
the National Academy of
Sciences of Ukraine

ELENA N.
GROMOZOVA


VOYCHUK

S.I.,
ZELENA

L.B.,
GRETCKEY

I.A.



is

the

biggest

scientific

research

center

in

the

area

of

systematic,

physiology,

biochemistry,

ecology

and

genetics

of

microorganisms,

biotechnology

and

general

virology

in

Ukraine

and

one

of

the

biggest

research

centers

in

the

former

USSR
.

Organized

on

the

31
st

of

May

1928

by

the

Dmitriy

K
.

Zabolotny
.


Directions of work

Study of fundamentals of
biologic activity of
microorganisms and viruses
aimed at its regulation

Study in ecology and systematic
of microorganisms and viruses,
strain selection and
development for the
biotechnological needs



the big collection of
nonpathogeneous

strains of microorganisms a lot of which
are used in molecular
-
biological investigations and biotechnology

Chromosomal aberrations
in cells of onion (
Allium
-
test)

Saccharomyces

cerevisiae

Schizosaccharomyces

pombe

Candida spp.

-0,053
-0,083
-0,571
0,722
-1,369
-3,30
3
p=,05
Effect Estimate (Absolute Value)
Temperature
Moisture
Flares C
Ap index
Flares M
Solar wind
speed
Specif ic growth rate
Solar wind speed
20
40
60
80
100
120
140
350
400
450
500
550
600
650
700
750
800
Solar wind speed
20
40
60
80
100
120
140
Growth Rate
R
2
=0.312
R=-0.559
p=0.000
Antibiotics

А
Р
-
index
,
nT

Wolf’s
number
(
R
)

Solar wind
speed
,
km
/
sec

Solar flux

(10,4
cm
)

Klotrimazole

0,09

-
0,51

0,61

-
0,53

Nystatin

0,24

-
0,45

0,35

-
0,37

Fluconazole

0,07

-
0,36

0,43

-
0,33

Metachromazy

reaction

29
33
37
41
45
49
53
57
61
65
69
73
77
52
60
68
0
10
20
30
40
50
60
70
80
90
100
Red
Blue
Type 3
90-100
80-90
70-80
60-70
50-60
40-50
30-40
20-30
10-20
0-10
19
23
27
31
35
39
43
47
51
55
59
63
67
44
53
62
0
200
400
600
800
1000
1200
Red
Blue
Type 1
1000-1200
800-1000
600-800
400-600
200-400
0-200
(PO
4
)
n

Volutin
grains
morphology

Volutin grains

“Dancing bodies”

Variations of Solar activity (in days,
by
Vladimirskiy

1994)

Periodicity
of metachromasy

Cosinor analysis

Fourier analysis

40
.
68
MHz
,

15W,
30
W,

EF = 90
-
92
V
/
m
,

MF = 1
.
25
-
2
.
2 А/
m

Used in medicine
for UHF
-
therapy

Used in medicine
for EHF
-
therapy

30
-
40

GHz
,

25mkT

Emit complex radiation of
varied frequencies

Used in
global

system

for

mobile

communications


DCS
-
1800MHz

10
-
1000
mkW
/cm
2

Yeast Population, Cell and
Subcellular

effects of EMFs

VHF

UHF

EHF

VDT

Growth rate

change

change

n/d

unchanged

Cell

cycle propagation

changed

n/d

n/d

n/d

Apoptosis

increase

n/d

n/d

decrease

Stress resistance

increase

unchanged

n/d

change

Enzyme

activity

change

change

n/d

unchanged

Adhesive properties

change

change

change

n/d

Membrane permeability

decrease

decrease

n/d

unchanged

Surface

potential

unchanged

unchanged

unchanged

unchanged

Treatment

Live cells, %

Untreated

VHF EMF

Optimal
conditions

92.5
±
1
.
7

93.1
±
1
.
4

Acidity
(
pH

2,7)

52.5
±
10
.
6

85.
5
±
6
.
5

Freezing
-
thawing

4
0
.
0
±
14
.
1

92.
5
±
3
.
5

Yeast Population

Effects


Cell surface effects

Control

Irradiated

Nano
-
hardness

E
nergy

dispersive

analysis

Yeast

Membrane permeability,
%

Untreated

VHF EMF

S.

cerevisiae

100

84

C.

utilis


100

76

S.

pombe


100

45

Membrane effects

Not changed:


Fatty acids composition

Dzetta
-
potential

Hydrophobicity

Genome stability effects of UHF
EMF

2 days exposition

Y
-
517

CRY

CNX

Y
-
517

CRY

CNX

Y
-
517

CRY

CNX

Y
-
517

CRY

CNX

50
mkW

m
2

200

mkW

m
2

1000
mkW

m
2

500
bp

1000
bp

100
bp

Power of exposition

Strain

6 weeks exposition

500
bp

1000
bp

100
bp

Control

Control

Irradiated 2 days

Irradiated 6 weeks

Fibroblasts (F)

F+Yeast

F+Irradiated

Yeast

Cell
-
to
-
cell interaction effects


Parameter

Yeast

Rats

Cytomorphological

changes

Yes

Yes

Metabolic activity changes

Yes

Yes

Physiological changes

Yes

Yes

Behavioral

changes

n/d

Yes

Genome changes

Yes

Yes

Effects
revertion

Not complete


n/d

Yeast
vsRats

comparative study


EMF

yeast cells

Signal amplification
with bio
-
stimulators

EHF
-
dielectrometry

Bio
-
stimulators are the
chemicals with known
mechanism of bio action

Main elements of the Biosensor based on
the EHF
-
dielectrometry

and Yeast cells

50
70
90
110
130
150
5
15
30
Bioluminescent index BI, %

time, min

1 µW
10 µW
100 µW
Biolunimescence

level of
Photobacterium

phosphoreum

under
EHF EMF

to be continued …

Our proposals for collaboration and future work:


1.
Comparative study of biological action of non
-
ionizing
and ionizing radiation in low doses;

2.
Development of biosensors to non
-
ionizing EMFs;

3.
Creation and evaluation of protection items against
negative action of non
-
ionizing EMFs;

4.
Researches on dosimetry, norms and standards of
biological action of non
-
ionizing EMFs.


Academic Writing Residency


http://www.rockefellerfoundation.org/bellagio
-
center/residency
-
program/academic
-
writing
-
residency