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22 Φεβ 2013 (πριν από 4 χρόνια και 3 μήνες)

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Analytical Services

May 19, 2009


Gretchen Grover

Lab Manager and Analyst


Christal

Wade

-

Principal Cryptosporidium
Analyst


Jonathan Oglesby
-
Cryptosporidium
Analyst


Rick Fowler



Environmental
Biotechnologist, Inorganic Analyst


Jana
Fattic



CWRS Associate Director, WATERS Director, Lab Analyst






Office
Associate:
Kristy
Mayes



Part Time:
Liz Thomas (Cryptosporidium
Technician), Samantha Kramer (Chemist)



Students
:
Kriss

Leftwich
, Daniel
Nedvidek
,
Catherine
Skees
, Jennifer
Ludden

(
Gatton

Academy), Ben Fowler (IC, AA student analyst)




Private


Total
Coliform
/E. Coli, Metals, Ions,
Atrazine


Wastewater


BOD, pH, TRC, DO, TSS, Fecal
Coliform
, Ammonia Nitrogen


Storm water


COD, Oil and Grease, TDS, TSS


Drinking
water


Total
Coliform
/
E.Coli


Source water


Cryptosporidium


Oil
Wells


pH, TDS, Specific Gravity, Chloride






Drinking Water Certification




Inorganic
Certification




Cryptosporidium





KY Microbiology Laboratory Certification Program
(KMLCP)



Renewed January 2009



Currently the largest (KMLCP) Certified Analytical
Staff in the State


Christal

Wade


Jonathan
Olglesby


Jana
Fattic


Rick Fowler


Gretchen Grover


Liz Thomas


Rick Fowler


Computer
-
controlled automated instruments


Operators: One staff supervisor, one student analyst



Ion Exchange Chromatography


Dionex

DX600 system with background suppression and
detection by conductivity or UV
-
Vis absorbance


Anions (F,
Cl
, Br, NO
2
, NO
3
, PO
4
, SO
4
) and
cations

(Li, Na, K,
Mg, Ca, and NH
4
+
), MDL <0.05
-
0.1
ppm



Atomic Absorption Spectroscopy


Varian AA240FS with graphite furnace and flame capabilities


Trace metals, MDL <0.0005
-
0.001
ppm


Gretchen Grover



pH, alkalinity, turbidity, TOC, TDS, odor




WATERS Lab received approval from EPA for the analysis
of Cryptosporidium on October 18,
2006.


Long Term 2 Enhanced Surface Water Treatment Rule
(LT2)



Currently approximately 70 clients from 8 states:

KY, TN, WV, VA, OH, NC, LA, MD



Crypto Program bills out ~$25,000 each month



Accept outside samples under LT2 and provides field
filtration apparatus






www.epa.gov/safewater/disinfection/LT2/lab_aprvlab
s.html



Mammoth Cave


Flood Pulse Event



City of Bowling Green


River & Cave System monitoring



Watershed Watch


Upper Green River watershed monitoring

(Turbidity and
Atrazine
)



TMDL


Analyzing samples for this project



University Research


Support WKU faculty as well as other university projects


Cave monitoring (
atrazine
, solids)


Wastewater treatment/
nanoparticles

(TOC)



DOW


Data Collection for Nutrient Criteria Development in the
Karst

Pennyroyal Region of KY


About 50 sites, 3 sampling events, in stream water quality data (pH, DO, temp) and lab analyses
(TP, NH3, TKN)







Projects completed


Mammoth Cave sediments bacterial
community analysis (2000
-
2008)


NCKRI artificial substrates bacterial
community analysis (2002
-
2008)


Final report for both projects combined into one
report published on the
Karst

Information Portal
and available at
www.wku.edu/~rick.fowler/mammoth


Information gained from these studies led to
discovery of fecal contamination resulting in
multimillion dollar infrastructure reformations
at MCNP visitor center

Projects completed
(continued)


Oregon Caves National Monument tourist trail
impact on microbial communities (2002
-
2006)


Identified numerous bacteria, fungi, and
protozoans

and discovered sulfur bacteria later observed by other
investigators now believed to be widespread in caves


Louisville Water Company


Ammonia Oxidizing Bacteria in distribution system


Acanthamoeba

in eyewash stations


KSU Aquaculture Research Center


DNA analysis of bacteria, algae, and fungi used to
evaluate conditions for optimal shrimp production

New projects


USDA Animal Waste Research Station


Develop methods for identification of animal origins of
fecal contamination from human and agricultural
sources


Study transport of pathogens from the surface into
subsurface aquifers due to agricultural practices on
karst

landscapes


Institute research and training programs at the newly
founded Crump’s Cave preserve near Mammoth Cave
National Park

Environmental Biotechnology



DNA
extraction from water, soil, membrane
filters, pure cultures, swabs, etc.


Yield of total DNA measured with
Qubit

fluorometer
, sensitivity 0.2 x 10
-
12
g



Quantitative
Real
-
Time PCR


Determines concentration of DNA from specific
organisms in a mixture, sensitivity <1
ppt



DNA
cloning and sequencing


Cloning permits segregation of individual species
comprising environmental microbial communities


DNA sequencing yields unique gene sequences for
identification of organisms

Bacteria

Fungi

Amoebae

Parasites

Algae

Zooplankton

Organisms analyzed with DNA

Scientific Outreach,
Presentations, and Workshops


National Cave and
Karst

Research Institute (NCKRI)


2007
-
present (Advisory board member)


China Environmental Health Project


2007 (International
Karst

Workshop,
ChongQing
)


Crump’s Cave Farm Day


2009 (Agricultural practices outreach and education)


International
Karst

Research Institute


2009 (17
th

International
Karstological

School,
Postojna
, Slovenia)


International Congress of
Speleology

ƒ
2009
(Kerrville, Texas)


Career Day for Middle School Students


Chemistry


Hach

LBOD


Konelab


Hach

Spec (COD, Surfactants, TRC)


GC MS


Microbiology


Microcentrifuge



Unregulated
Contaminant Monitoring Rule (UCMR2)



Organics
(Work toward a GC MS/MS and HPLC
)



Direct the
Chemical Laboratory Certification Program for
Kentucky



Voluntary
Wastewater
Certification



Pick up service



Consultation services



Sample collection services