here.

raviolirookeryΒιοτεχνολογία

2 Οκτ 2013 (πριν από 3 χρόνια και 10 μήνες)

130 εμφανίσεις

Gene expr
ession

profiling


cDNA normaliz
ation
-

SNP Analysis
-

RNAseq
-

qRT
-
PCR




S
ample
-
shipping form for RNA
and DNA
samples


Please ship samples
in RNAse
-
free buffer
on dry
-
ice in
parafilm
-
wrapped and
sealed tubes.

Tubes should be
firmly placed in an extra
container

within
the dry ice
-
box.



Quotation number:
____________________________


Email address of responsible person:____________________________


Sample description and preferred names:

Please define biological replicates by “BR”.


Number

Sample description

(optional)

Preferred abbreviation



(max 6 characters)

Quantity

1




2




3




4




5




6




7




8




9




10




11




12





Alternatively and for more than 1
2

samples, please send an excel file to
“customerservice@genxpro.de”



Quantification method:

(please define)
__________
__
_____________________



P
urification method:


Column





Ethanol precipitation





Others




(please define)
__________
__
_____________________


DNAse digested
(for RNA)



yes



no




RN
Ase digested
(for DNA)



yes



no





Pelleted






yes



no






Buffer/liquid:




(please define)
__________
__
_____________________




Gene expr
ession

profiling


cDNA normaliz
ation
-

SNP Analysis
-

RNAseq
-

qRT
-
PCR



Gel picture or Agilent 2100 trace
:
please paste here or send via e
-
mail

to
Customerservice@genxpro.de

including the quotation number.














For b
ioinformatics
:



Annotation:

Please define Database to which the sample shall be
annotated. If no database is st
ated, NCBI
-
refseq databases or
TIGR
-
gene indices will be used

whenever possible
.


Preferred database (URL):


Data storage:
Data will be stored 6 Month
after delivery. Please state if additional storage on
an external hard
-
drive
for
an additional fee of 125 EUR

is required:
yes



no





F
or gene expression analysis
:


Please define the samples which should be compared in pairwise comparisons using the
preferred abbreviations from the above tables.


If “in
silico” pools of samples shall be compared, please define the pools by parentheses.


Biological replicates
(BR)
will be treated using negative binomial statistics in pairwise
comparisons for calculations of p
-
values for differential expression.



Compariso
n

Samples

1

Samples

2

1



2



3



4



5



6



7



8



9



10



11



12




Alternatively and for more than 12 comparisons, please send an excel file to
customerservice@genxpro.de