Genetic Disorders and Gene Therapy Today

rapidparentΒιοτεχνολογία

12 Δεκ 2012 (πριν από 4 χρόνια και 8 μήνες)

205 εμφανίσεις

Yesterday:

Genetic Disorders

and

Gene Therapy

Today:

Genetic Engineering

What does Genetic Engineering mean to You?

Overview of Genetic
Engineering


What are the differences between
cloning, recombinant DNA, and
genetic engineering?


What are the tools we use for
genetic engineering?


Plasmids


Restriction Enzymes


DNA Ligase


Cloning Involves Making Identical
Copies

“Cloning” can mean several things:


To make many identical copies of a
DNA molecule or a particular stretch
of DNA (DNA cloning or molecular
cloning).


To replicate an entire organism
(reproductive cloning).

Recombinant DNA


Recombinant DNA is when two or
more pieces of DNA that don’t
normally go together are
combined
.


Genetic Engineering


Genetic Engineering is the process
of using recombinant DNA
technology to genetically alter or
create new organisms.

Recombinant DNA Technology
Tools


Plasmid Vectors


To move DNA from one organism to
another.


Restriction Enzymes


“Molecular Scissors” to cut DNA in
specific places.


DNA Ligase


DNA “glue” to stick pieces of DNA
together.

Plasmids

Small, circular pieces of
“extra” DNA found in bacteria.


Plasmids often carry

antibiotic resistance.

Plasmids

Restriction Enzymes:

Molecular Scissors

A restriction enzyme
(RE) is a specialized
protein that cuts DNA
in a very specific
place.


Different REs cut at
different places along
the nucleotide
sequence.

Cell DNA

DNA Ligase (Glue)

Recombinant DNA

DNA fragment ligated
(inserted) into vector
creating recombinant
DNA molecule.

Today


Gene Therapy Vector Engineering
Project.


Use paper models to simulate the
process of engineering your
recombinant gene therapy vector.


DNA sheet for the therapeutic gene
to treat the genetic disease you were
assigned.


DNA sheet for the vector you chose
to treat the disease.


Materials


In lab folder (one per group):


Activity Instruction Sheet (WHITE)


Answer/Data Sheet (WHITE)


Restriction Enzyme Sheet (GREEN)


Get from me (one per group):


Vector DNA sheet (PINK)


Therapeutic DNA sheet (BLUE)


Other: scissors/glue stick