Gel Electrophoresis

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16 Δεκ 2012 (πριν από 4 χρόνια και 6 μήνες)

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Biology


The Continuity
of Life



Objective 3 & 4


Describe the various ways genetic
engineering can change the
organisms around us.


Demonstrate how DNA can be
manipulated and used by science.

Gel Electrophoresis


“Gel”

think jello


“electro
-


uses electricity



-
phoresis”

root for ‘movement’




Purpose:


To separate molecules according to their
size

Gel Electrophoresis


Components & their Jobs…


Electricity


Moves the molecules through
the gel


Agarose Gel


provides resistance for
molecules to move through


Buffer


allows electricity to get to the gel


Loading dye


allows you to see invisible
DNA strands

RFLP: Gel Electrophoresis

Direction

of Migration

Larger fragments

move more
slowly;

smaller fragments

move more rapidly

Negative

Positive

Gel Electrophoresis


Uses…ALWAYS used to separate
molecules due to size!


DNA


cut DNA into pieces and then see
if samples match



Proteins


can be used to see if a protein
is present in a sample (food poisoning
cases, “imitation crabmeat”?)

C

S

R

C

I

E

M

N

E

E

DNA Fingerprint in
Forensics

1

2

3

4

5

6

7

Suspects

Suspects

Q: Which suspect

should be

indicted?

A: #3 is prime

suspect