Buy the amazing GloFish! - MStephens

pumpkincentersulkyΒιοτεχνολογία

16 Δεκ 2012 (πριν από 4 χρόνια και 10 μήνες)

204 εμφανίσεις

Have you ever been sitting in your room at night thinking to
yourself “Wow it’s really dark in here, I wish I had a night light,
but the ones you plug into your wall are so lame!” If so, don’t
worry about being lame any longer because there’s a sweet new
genetically modified species on the market that will make you so
cool you’ll practically be a cucumber. They’re called
GloFish
.


Using restriction enzymes, gel electrophoresis,
cDNA
, DNA sequencing, and PCR, science has
managed to create these magical creatures.


Gel electrophoresis machine

Restriction Enzymes


Restriction enzymes cut DNA at specific recognition
sites and can essentially allow scientists to bind certain
sequences with other sections of DNA, making genetic
engineering possible. By using these, scientists have
discovered a sequence of DNA that codes for the glow
in the dark feature the
fishies

have.


Gel electrophoresis


Gel electrophoresis separates large DNA on the basis
of the rate of movement through and
agarose

gel in an
electrical field, allowing scientists to see the exactly
sequence of bases in DNA strands. This is important in
the glow
-
in
-
dark jellyfish
-
making process because
scientists need to be able to know the exact genetic
makeup of the jellyfish before they can modify its
bases to make it glow.


Electrophoresis
taking place in the
agarose

gel.

Complementary DNA


cDNA

(complementary DNA) are DNA molecules that
are made using mRNA template. Scientists create
cDNA

libraries which provide extensive information
on certain genes that are responsible for the
specialized functions of a specific cell. This is helpful
when trying to determine what genes will make the
fish glow.


DNA sequencing


DNA sequencing is exactly what it sounds like, the
complete sequence of nucleotides for every single
chromosome in a cell. It is important because in order
for scientists to genetically modify organisms they
must first understand the original sequence of that
organism’s genes.


An small part of a sequence of DNA.

Polymerase chain reactions


PCR, or polymerase chain reaction, is used to amplify
or make copies of DNA being sampled very rapidly.
While the
GloFish

were being made, scientists had to
do many tests and trial runs to make sure that they
would work and were safe so they used PCR to make
lots of copies of the DNA from the fish to test it.


So, buy your
GloFish

today!

They’re great as night lights and kids love them