MARINE ADHESIVES: MARINE ADHESIVES: FROM BIOLOGY TO BIOMIMETICS FROM BIOLOGY TO BIOMIMETICS

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14 Νοε 2013 (πριν από 3 χρόνια και 4 μήνες)

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Patrick Flammang
Universityof Mons -UMONS,
Marine BiologyLaboratory,
Mons, Belgium
MARINE ADHESIVES:
MARINE ADHESIVES:
FROM BIOLOGY TO BIOMIMETICS
FROM BIOLOGY TO BIOMIMETICS
COST StrategicWorkshop on PrinciplesandDevelopmentofBio-inspiredMaterials
14 April2010
Challenges for new adhesives
enableattachmentto a varietyofsurfaces, alsoin fluid
environments, for a broadrange ofapplications
beenvironment-friendly
bereusable, allowingmultiple attachmentsanddetachments
bereversible(or switchable), sothatdetachmentcanoccur
atwillwithnegligibleforce
Biologicaladhesives
Biological adhesives: from biology to biomimetics
New COST Action TD0906
Sofar 10 countries (AT, BE, CH, DE, FI, FR, IE,IT, PT, UK)
have acceptedtheMoU.
Kick-offManagement Committeemeeting willtakeplace on
May 18th.
Poster #1
The main objectiveisto gain new understanding relating to the mode of
action of biological adhesives so as to facilitate the development of
synthetic counterparts with improved function.
Biologicalattachmentdevices
Patternedadhesives
Biologicalglues
Barnacles
Types
Types
of
of
adhesion
adhesion
1)Permanent adhesion
by secretionofa cement
2)Non-permanentadhesion
by secretionofa visco-elastic
adhesive
3)Instantaneousadhesion
allowingveryfast
attachment
Mussels
Gastropods
Seastars
Combjellies
Seacucumbers
Permanent adhesion
Non-permanent adhesion
Instantaneousadhesion
Mussels
Seastars
Seacucumbers
All biologicalglues from
marine invertebratesare
basedon specializedproteins,
the marine adhesiveproteins
Post-translationalmodifications ofmarine
adhesiveproteins
1)Phosphorylation ofserine residuespSer
2)Hydroxylation oftyrosine residuesDOPA
Adhesive
Adhesive
motifs
motifs
Currentstate ofknowledge
Onemodel systems hasinspired most biomimetic approaches for
biological glues:themussel byssus.
Waite(2002), IntegrCompBiol42:1172-1180
Waiteet al. (2005), J Adhesion81: 297-317
fp1
Thread
fp3 and5
Plaque
fp2
Collagen
Substratum
fp4
Musseladhesiveproteins
Synthesis
Extraction
Biotechnology
Production
Production
of
of
biomimetic
biomimetic
or
or
bio
bio
-
-
inspired
inspired
adhesives
adhesives


Tissue
Tissue
adhesives
adhesives
for
for
in vivo
in vivo
use
use
Applications


Adhesive
Adhesive
coatings
coatings
to
to
functionalize
functionalize
surfaces
surfaces
and
and
interfaces (
interfaces (
e.g.
e.g.
, in composite
, in composite
materials
materials
)
)




Threemodelsare investigatedin ourlaboratory
Thetubeworm
Permanent adhesion
Theseastar
Non-permanentadhesion
Theseacucumber
Instantaneousadhesion
Permanent adhesion: Tubeworms
Sabellariaalveolata
, thehoneycombworm,
isa tube-dwellingpolychaete
10 µm
1 µm
Thecementisa complexcomposite material
1 µm
200 µm
Cementcell
bodies
Cementcell
processes
Lobe ofthe
building organ
Thebuilding organofthetubeworm
Sabellaria alveolata
5 µm
5 µm
Sa-3, fragment of
178 aminoacids,
75% serine residues
(phosphoserine)
Sa-1, fragment of
217 aminoacids,
repeatedsequences
richin tyrosine
residues(DOPA)
The adhesiveproteinsof
Sabellariaalveolata
Theseastar
Asteriasrubens
canattachstronglybut temporarily
to thesubstratum witha multitude of tinyappendages,
theTUBE FEET
Non-permanentadhesion: Seastars
AC1: Type 1 adhesivecell
AC2: Type 2 adhesivecell
SC: Sensorycell
DAC: de-adhesivecell
Tube feetrelyon a duo-glandadhesivesystem
FM: Fibrillarmaterial
HL: Homogeneouslayer
Su: Substratum
DE: Disc epidermis
FM: Fibrillarmaterial
HL: Homogeneouslayer
Su: Substratum
Seastar footprints
Characterization
Characterization
of
of
adhesive
adhesive
footprints
footprints
Novelproteins
Seastar footprintproteins(Sfps)
40
de novo
generatedpeptide
sequences
Glycoprotein
Phosphoglycoprotein
Instantaneous
Instantaneous
adhesion
adhesion
:
:
Sea
Sea
cucumber
cucumber
Severalspeciespossessa peculiardefencesystem, theCuvieriantubules
Morphology
Morphology
of
of
Cuvierian tubules
Cuvierian tubules
Adhesiveepithelium
Connective tissue
layer
Lumen
ProteinsextractedfromCuvieriantubule glue prints
Somecontaminant proteins
Severalnovelproteins
TheadhesiveofCuvierian tubules contains
polyphosphoproteins
Adhesive
epithelium
Caseincontrol
(milkpolyphosphoprotein)
Proteinsextracted
fromtheCuvierian tubule glue
Marine adhesiveproteins
Synthesis
Biotechnology
Production
Production
of
of
biomimetic
biomimetic
or
or
bio
bio
-
-
inspired
inspired
adhesives
adhesives
Noveladhesivesor coatings
Acknowledgements
Acknowledgements
Elise Hennebert, Aurélie Lambert, Pierre Becker
Pascal Damman, UniversityofMons, Belgium
Joseph Martial, UniversityofLiège, Belgium
HerbWaite, UniversityofCaliforniaatSantaBarbara, USA
RuddyWattiez, UniversityofMons, Belgium
Support