Microbiology/Biotechnology II Course Syllabus - Plainwell Schools

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12 Φεβ 2013 (πριν από 4 χρόνια και 8 μήνες)

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Microbiology
/Biotechnology

I
I

Course Syllabus


Course Description

This course is a continuation of Biochemistry/Biotechnology I. It will be lab based and focus on
working with microorganisms and the production of biotechnology products.


Textbook:

Biotec
hnology, Science for the New Millennium
, Daugherty, Ellyn

Will also be using portions of:


Biology,
Miller Levine





DNA Science, a first course,
Cold Springs Harbor Press





Biology,
Campbell/Reese


Notebooks:
Students are required to obtain the follo
wing notebooks


Bound composition notebook (must be bound with thread) for lab


Three ring binder


Spiral notebook for lecture notes


Requirements:


Use information from your biochemistry/biotechnology I notebook to conduct basic
biotechnology lab techniq
ues.


Come to class prepared


Textbook, notebooks, writing utensils and assignments must
be brought to class each day.


Students without the proper materials and dress will not be allowed to participate in lab

activities. Students must have long pants

and closed toed shoes for all labs. Students

must have official lab notebook for all labs.



Students are required to follow all safety procedures during labs. Violations will result in

a zero for the lab and possible dismissal from class.



All labs
missed due to excused absences must be made up after school.



Labs missed due to unexcused absences will not be made up and a zero will be given.


Assignments/labs:


Late assignments will
not be
graded (students will receive a 0)



Assignments include: r
eadings/notes, practice sheets, review packets and lab



write
-
ups.


Labs: Only material in official lab book will be graded.



Grading:


Marking period grade (80 % of total trimester grade)



Laboratory Notebook



Tests (lab and background information)


Exam Grade: (20% of total trimester grade).



Final exam will include both lecture and

lab questions.



Grading Scale: 93


100

A


77


79

C+




90


92


A
-


73


76


C




87


89


B+


70


72


C
-




83
-

86


B


67


69


D+




80


82


B
-


63


66


D








60


62


D
-

Part I: Working with microorganisms


Media for growing bacteria


Culturing bacteria


Broth culture


Streak plate

Growth of bacteria


Graphing growth rate


Measuring with spectrophotometer


Part II:
DNA to Protein:


Overnight suspen
sion

Mid
-
log

Preparing competent cells

Transforming bacteria

Purification of protein product

Identification of product

Mini prep

Recombination of bacterial genes


Part III: (intermixed with part 2) Identification of bacteria


Staining Techniques:

Simple s
tain

Gram stain

Spore stain


Part IV:
Bacterial metabolism
:


Fermentation of sugars



Research Question:
How can microbial techniques and genetic recombination be used to
produce biofuels?