BioTechnology: Web Quest

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23 Οκτ 2013 (πριν από 3 χρόνια και 9 μήνες)

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BioTechnology: Web Quest


Name______________________________Per.___

Part 1
-

Manipulating Genes

Go to
http://www.dnai.org/b/index.html


Read the introduction.

What were some
questions scientists asked in the 1970’s
regarding the


genes in DNA?

1. Questions
_____________________________________________________________________
_____________________________________________________________________
_____________________________________________
________________________

Click on Revolution

Summarize the problem

2. Summary of problem

_______________________________________________________________________
_______________________________________________________________________

Click on Pieces of th
e Puzzle

(top menu bar)

Learn about each piece of the puzzle by
clicking on it. Read the text and view the animations. Summarize what you have learned. You do
not have to go in the order listed below.

3.
Restriction Enzymes




4.
DNA ligation




5.
The First Recombinant DNA




6.
DNA Transformation
-



Click on Techniques

(bottom of page)

Click on Cutting & Pasting

(top of page)

Click
on the Cutting & Pasting DNA button
.

Read the text and view the video.

7
.

Make 3 sketches;
a)

Before DNA is cut

b)

After it is cut, and
c)

after it is pasted together.
(Include nitrogen bases and which type of enzyme is used at each stage.)

a)





b)






c)




Click on Recombining DNA
.

Read the text and view the video

8.
What is a plasmid? ________________
________________________________
____

What organisms have plasmids?

____
__________
______

9.
Summarize the technique developed in the 1970’s in which a DNA fragment is added to a

plasmid.
_________________________________________________________________
__
_____________________________________________________________________
_______________________________________________
________________________
_______
_________________
______________________________________________
_

*
Check out the interviews with the
scientists that made these discoveries.

If you are
interested continue with the other techniques involved in manipulating DNA


.

Part 2


Cloning and Gene Therapy

Go to
http://learn.genetics.utah.edu

Click on
Cloning

at the bottom of the page
under the
Genetic Technology

tab.

1.

The interactive tutorial
What is Cloning?
will review the process of cloning and it’s
techniques. Define below what is
a
clone
?

_____________________________________________________________
__________

2. Click on
Click and Clone
to create a mouse clone. Write the steps involved in cloning your
mouse below.

_______________________________________________________________________

_________________________________________________________________
______

_______________________________________________________________________

_______________________________________________________________________

What color coat will your mouse clone have?__________________________

Part 3


Gene Therapy

Click on
Gene Therapy

under the
Genetic Technology

tab. Then
click on
Space Doctor.

1. Click on
tools of the trade

and review the Vector Toolbox.
What can be used as a vector
and
what is the role of a vector in genetic engineering?

_______________________________________________________________________

_______________________________________________________________________

2.
Click on
Gene Delivery: The Key to Gene Therapy.
Review the information presented.

3.
Become the Space Doc
tor and design a gene therapy for one of the three given patients. Fill
out the following:

Patient__________________________ Disease_________________________________

Tissue targeted ____________________________

Vector chosen (
consult the toolbox if you
need a refresher
) _______________________________

Why this vector?__________________________________________________________

_______________________________________________________________________

Was your therapy
in vivo

or
ex vivo
?
Circle one

Was your therapy successful? __________

Why or Why not?

__________________________________________________________

_______________________________________________________________________

____________________________________________________________________
___

List all the vectors that worked for this gene therapy_______________________________

_______________________________________________________________________


Part 4 Applications

Go to
http://www.dnai.org/d/index.html

You have already investigated one
application of biotechnology in the above “fingerprinting” activity. In this
section other applications of the technology are explained. Choose between
the Genes & Medicine or the Human Origins modules and explore it. Pick an
area of interest for you in ei
ther module and write a short paragraph about
it. What did you learn?







3


Part 5


Ethics

Go to
http://www.thetech.org/exhibits/online/genome/thread3.html

In this
section you will explore some ethical issues associated with biotechnology.

Click on each dilemma
. Read the dilemma and decide what you would do. Put
your answer on the appropriate line.

1.

Be a
parent________________________________________________
_________.

2.

Be a judge

____________________________________________________
__________
_.

3.

Be a doctor

____________________________________________________
___________.

4.

Be a voter

____________________________________________________
____________.

5.

Be a patient

____________________________________________________
___________.



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