Faculty of Chemistry and Chemical Technology, Aškerčeva 5 ...

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Faculty of Chemistry and Chemical Technology, Aškerčeva 5, Ljubljana


30-0174-EN
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1. SUBJECT: MICROBIOLOGY

Code: 30-0174
Credits: 6.5
Form of teaching: 90; Lectures 45, Seminars 0, Practicals 45 hours
Programme: University Undergraduate Programme Biochemistry

2. OBJECTIVES

The lectures are specifically designed for the students of biochemistry to acquire knowledge
about the complexity of living organisms, became aware of biodiversity of microbial species
that developed during evolution and learn about their metabolic differences. Part of lectures is
devoted to presentation of their relationship with human beings, both from negative
(pathogenicity) and positive (biotechnology) aspects.

3. SYLLABUS

Lectures:
Introduction to microbiology: Short history. Definition of microbial cell. Specific properties
of Prokaryotic and Eukaryotic cells.
Microbial growth: Definition of pure culture. Environmental effects on microbial growth.
Growth cycle. Adaptation to extreme conditions. Microbial growth control.
Microbial evolution and systematics: Origin of microbial species during evolution.
Taxonomy of Archaea, Bacteria, Protozoa, Algae, and Fungi. Viruses.
Microbial genetics: Genetic mechanisms as a driving force of evolution.
Metabolic diversity: Phototrophic life. Chemolithotrophy, energy from the oxidation of
inorganic energy sources. Anaerobic life. Hydrocarbon oxidation and the role of oxygen in
the catabolism of organic compounds. Nitrogen fixation.
Microbial habitats: Microbial ecosystems. Carbon and oxygen cycles. Other key nutrient
cycles. Bioremediation. Xenobiotics. Microbial interactions with plants.
Human-microbe interaction: Beneficial microbial interactions with humans. Harmful
microbes. Virulence factors and toxins. General host defence mechanisms.
Industrial microbiology: Industrial microorganisms. Growth of microbial cells in
bioreactors. Biomass as a product. Primary and secondary metabolites. Biotransformation.
Heterologous proteins. Down-stream processes.











Practicals:
• Substrates for growth of microorganisms – sterilisation.
• Inoculation techniques.
• Determination of the number of viable microbial cells in solution.
• Microbial culture collections.
• Mutations – selection of auxotrophic mutants.
• Screening techniques.
• Protoplastation.
• Desintegration of microbial cells.
• Determination of cellular volume.

4. PREREQUISITES

Before attending the lectures on Microbiology students should obtain a background in
Cytology and basic Biochemistry. The studies of Microbiology are essential for
understanding lectures on Biotechnology, Immunology, Molecular biology and Recombinant
DNA techniques.

5. RECOMMENDED TEXTBOOKS

MAIN TEXTBOOK:
• M.T. Madigan, J.M. Martinko, J. Parker: Brock's Biology of Microorganisms, Prentice
Hall, 10
th
Edition. 2003.
• G.J. Tortora, B.R. Funke, C.L. Case: Microbiology, The Benjamin/Cummings Publishing
Company, Inc., 8
th
Edition, 2003.
• M. Legiša, M. Benčina: Vaje iz Mikrobiologije, Založba UL FKKT, 2002.

ADDITIONAL LITERATURE:
• Moat, J.W. Foster, Microbial Physiology, J. Willey and Sons, 1995
• S. Bengston, Early Life on Earth, Columbia University Press, 1994.

6. FORM OF ASSESSMENT

The students must pass a written exam from both lectures and practicals.



PREPARED BY: Doc. Dr. Matic Legiša

DATE: 17. 05. 2003

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