Network Configuration of an MSD Trap Acquisition PC

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Network Configuration of an MSD Trap Acquisition PC
(Connection to Instruments and TCP/IP or Novell NetWare
G2440AA/CA/DA LC/MS Ion Trap System

General Network Configuration:

1) The LC/MSD Trap Acquisition PC is equipped with two Network Interface cards (LAN-

2) The first LAN-card is for connection to the “house network” (via 10Base-T, 100Base-TX, or
10-Base2 on some PCs). The IP address for this card needs to either be specified or can
be dynamically assigned (e.g. obtained by a DHCP server). By default, TCP/IP and
NetBEUI are configured as network protocols for this card. For connection to a Novell
Network, please follow the instructions in the separate chapter.

3) The second LAN-card is dedicated for instrument communication with the MSD Trap MS
and the Agilent 1100 HPLC system. This separate network should NOT be connected to
the house net for two reasons:

a) to avoid problems in the communication between the PC and the MS and HPLC in case
of heavy network traffic or problems on the house net (e.g. to avoid interruption of
overnight sequence runs)
b) to allow for easy installation of the LC/MSD Trap-NT software including automatic
communication configuration without customer input (see item 5, BootP Server)

4) IMPORTANT: The following “TCP/IP Properties” MUST be set for the instrument LAN card
(Adapter 2). They can be accessed by choosing “Start > Settings > Control Panel >
Network”. On the “Protocol TAB”, highlight “TCP/IP Protocol” and choose “Properties”.
In the “Adapter:” pull-down menu, choose Adapter [2]:

a) IP Address TAB: Select “Specify an IP address“, and enter exactly “” as
IP Address, “” as “Subnet Mask” but do NOT enter an IP address under
“Default Gateway” (which keeps this subnet isolated).
This document is believed to be accurate and up-to-date. However, Agilent Technologies, Inc. cannot assume responsibility for the use of this
The information contained herein is intended for use by informed individuals who can and must determine its fitness for their purpose.


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b) DNS TAB: You can enter information here which is needed for connection to the house
net over Adapter 1 (house net LAN card). It will not negatively effect function of Adapter
2 (instrument LAN card).
c) WINS Address TAB: Do NOT enter any IP address under “Primary” and “Secondary
WINS Server”.
d) Routing TAB: Do NOT check the “Enable IP Forwarding” checkbox.

5) BootPServer:

When the LC/MSD Trap-NT software is installed, a “BootP Server” is installed and
automatically configured on the Acquisition PC in order to allow communication between
the PC, the Mass Spec and the HPLC, which all have a Network Interface Card (in the PC
this would be the “instrument LAN-card”, Adapter 2). The “BootP Server” is a program
which assigns IP addresses to the “MAC addresses” (LAN Hardware address) of the
Network Interface Cards. The MAC addresses are 12-digit numbers, which are world-wide
unique, the first 6 digits being characteristic for the manufacturer or distributor of the LAN-
device. Usually in the BootP Server a table has to be set up that individually assigns each
12-digit MAC address with an individual (world-wide unique) IP address, if this device is
connected to the internet over the house network. When a LAN-device is switched on (here
the MS or HPLC), it sends out a request into the network for getting an IP address
assigned. For the MSD Trap software the BootP Server is set up such that it listens only on
the separate instrument network (Adapter 2 in the PC, IP address for
requests, it only checks for the first 6 digits of the MAC address and then assigns fixed IP
addresses to the MS ( and HPLC ( Thus, communication
can be established without having to know the unique full 12-digit MAC address of each
individual MS or HPLC.

The current HP batches covered are: 0060b0xxxxxx, 080009xxxxxx, 001083xxxxxx,
100090xxxxxx, 000063xxxxxx, 00060dxxxxxx.

The MAC address on the HP J4100A JetDirect Card can be found on a sticker - usually on
the underside of the circuit board, showing a barcode and two 12-digit numbers above and
below. The number with the prefix "AD:" IS the MAC address, the number with the prefix
"TD:" is NOT the MAC address.

a) This convenient setup requires, that the instrument LAN-card (Adapter 2) is separated
from the house-net (see item 4a above).
b) If there should be a new batch of MAC addresses out on an HP JetDirect card, the
BootP Server needs to be configured manually (see next page).
c) Only ONE BootP Server can be active on a network. Do not install the “CAG BootP
Server” which is provided from HP with on the ChemStation Installation CD (uninstall if
necessary by selecting “Start > Settings > Control Panels > Add/Remove Programs”).


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Manual Configuration of the BRUKER BootP Server:

Start the Bruker BootP configuration software used by Bruker and found as C:\Program
Files\Bruker Daltonik\Esquire\Network\udpnetctrl.exe. Upon execution of this program the
following display will be seen:

There are two hosts configured here. One is the LC/MSD Trap (Esquire), which should never
have to be reconfigured since the MAC address on the GEPS card is known explicitly to


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Bruker. The second host is HPLC configured to the MAC addresses of the JetDirect card
installed on one of the LC1100 modules. According to item “5)” above, the BootP is configured
for MAC addresses of three different HP batches. However, a JetDirect card may be installed
on the LC1100 that contains a MAC address which has its first six characters differing from
those first six characters in any of the three HP batches.

In this case, the BootP software must be manually configured for the different JetDirect card.
The next step would be to highlight the HPLC entry in the “BootP Device Configuration”
window of the view shown above. Click on the “Remove Host” button to change to a view like
that shown below.


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Read the MAC address on the JetDirect card to be used in the LC1100. Be sure to turn of the
power to the appropriate module before removing or installing this card. The address is
located on the side of the card that faces down when installed and starts after the letters “AD.”
Click the button “Add Host” to see the following:

Replace with your
JetDirect card MAC

Add the information shown above, using the entire 12 characters of the MAC address of your
JetDirect card instead of the one shown above. Use the IP Address and IP Netmask shown
above. Click the OK button to see the following display:


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Actually shows your
JetDirect card MAC

Note: After clicking OK to close this utility, replace the JetDirect card into the LC1100 module,
power cycle that module and wait about a half-minute before starting the ChemStation and the
LC/MSD Trap software using the “LC/MSD Trap Online” desktop icon. Let the module initialize
and request an IP address before starting the control software.


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Configuring the “house" LAN card (Adapter 1) for connection with a
Novell Network:
1) Choose “Start > Settings > Control Panel > Network”.
2) On the “Protocols” TAB, verify that NWLink IPX/SPX Compatible Transport and
NWLink NetBIOS are NOT listed as Network Protocols (only NetBEUI Protocol
and/or TCP/IP Protocol should be listed). If this is the case, click the Add… button.
3) In the Select Network Protocol window, select NWLink IPX/SPX Compatible
Transport and click OK. You will be asked to point to the \i386 directory, which is
e.g. located on your Win NT 4.0 Installation CD. Then click OK to proceed with the
4) After installation of the protocol, you should now see NetBEUI Protocol, NWLink
IPX/SPX Compatible Transport, NWLink NetBIOS and TCP/IP Protocol listed as
Network Protocols.
5) Highlight NWLink IPX/SPX Compatible Transport in the list and choose
6) In the NWLink IPX/SPX Properties window, select the Adapter (1) in the Adapter:
Drop-down menu, which is the LAN-card dedicated to the in-house network.
7) On the General Tab, either leave the Frame set to Auto Detect or select the
appropriate setting for Frame Type and Network Number, if specified by your IT
department. Choose OK to leave the NWLink IPX/SPX Properties window.
8) Then, go to the Services TAB in the Network Control Panel and click the Add…
9) Select the Client Service for NetWare and click OK. Again point to the \i386
directory on your Win NT 4.0 Installation CD and click OK to proceed with the
IMPORTANT: The “Client Service for NetWare” selected here is the Client provided
by Microsoft with Windows NT 4.0. DO NOT use “NetWare Clients” provided by
Novell. Since various versions caused problems, Novell Clients are NOT
SUPPORTED on the LC/MSD Trap Acquisition PC.
10) Then select the Bindings TAB in the Network Control Panel and from the Show
bindings for: drop-down menu, select all adapters. Click on the two “+” boxes left of
the two adapters to expand the view. Then, for the SECOND adapter (2), which is the
LAN-card dedicated to the instruments, highlight the NWLink IPX/SPX Compatible
Transport and select Disable. A red symbol will indicate that the protocol is
11) Then, disable the WINS Client (TCP/IP) and NetBEUI (if existing) in the same way.
COMMENT: When installing network protocols in Win NT, the protocols by default
will be bound to all installed network cards. The instrument LAN-adapter (2) is using
TCP/IP only and to avoid conflicts, all other protocols should be disabled.


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12) Close out of the Network Control Panel. You will be asked to reboot your computer.
IMPORTANT: At this point, do NOT select to reboot (click NO). Instead, reinstall
Windows NT Service Pack 4 first, because components from the original Windows
NT CD have been installed. It is important to use Service Pack 4 and not any lower
or higher versions until they are officially tested and supported with the LC/MSD
Trap-NT software!
13) Reinstall Windows NT 4.0 Service Pack 4 by inserting the Windows NT 4.0 Service
Pack CD, which shipped with your LC/MSD Trap-NT software upgrade kit.
14) If your CD-ROM drive is set to “Autorun”, an HTML document will be launched in your
web browser (choose G2710AA LC/MSD ChemStation software.under Section
Contents and follow the instructions). Otherwise, launch the Windows Explorer,
double-click the Spsetup.bat command in the root directory of the CD, and follow the
15) At the end of the installation, reboot your PC as requested.

G2440AA G2440CA G2440DA